Estudio inmunohematológico de la enfermedad hemolítica ABO

Cid Vidal, J.; Elies Fibla, E.

doi:10.1016/S1695-4033(00)77452-2

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Cid Vidal, E. Elies Fibla" "autores" => array:2 [ 0 => array:3 [ "nombre" => "J." "apellidos" => "Cid Vidal" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "*" "identificador" => "cor0005" ] ] ] 1 => array:2 [ "nombre" => "E." "apellidos" => "Elies Fibla" ] ] "afiliaciones" => array:1 [ 0 => array:1 [ "entidad" => "Centre de Transfusió i Banc de Teixits. Tarragona" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "*" "correspondencia" => "CTBT-Tarragona. Dr. Mallafré Guasch, 4. 43007 Tarragona" ] ] ] ] "titulosAlternativos" => array:1 [ "en" => array:1 [ "titulo" => "Immunohematologic study of ABO hemolytic disease" ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2000-02-29" "fechaAceptado" => "2000-06-30" "PalabrasClave" => array:2 [ "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec186429" "palabras" => array:4 [ 0 => "Enfermedad hemolítica ABO" 1 => "Eluido" 2 => "Prueba de la antiglobulina directa" 3 => "Hematócrito" ] ] ] "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec186428" "palabras" => array:4 [ 0 => "ABO hemolytic disease" 1 => "Eluate" 2 => "Direct antiglobulin test" 3 => "Hematocrit" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "es" => array:1 [ "resumen" => "ObjetivoLa enfermedad hemolítica ABO puede manifestarse cuando existe incompatibilidad de grupo ABO entre la madre y el hijo. El objetivo del presente estudio fue analizar la incompatibilidad serológica ABO presente en nuestro centro, comparar 2 técnicas immunohematológicas para su diagnóstico serológico y evaluar la repercusión de la incompatibilidad ABO en el hematócrito de la sangre de cordón umbilical. MétodosSe realizó el grupo ABO y Rh(D) en 881 muestras de sangre materna y de sangre de cordón. Con esta última se realizó la prueba de la antiglobulina directa (PAD), el eluido y el microhematócrito. ResultadosLa incompatibilidad ABO se dio en 134 (15,3%) casos. Ésta se detectó con la PAD y el eluido en un 31,6 y 68,4% de los casos estudiados, respectivamente (p = 0,001). El grupo con incompatibilidad ABO presentó unas cifras de hematócrito inferiores al grupo sin incompatibilidad, con una diferencia estadísticamente significativa (44 ± 7,8% frente a 46,5 ± 6,5%; p = 0,029). ConclusionesAunque la enfermedad hemolítica ABO clínicamente significativa es rara, la incompatibilidad ABO se detecta con las técnicas serológicas comúnmente utilizadas y es causa de cierto grado de hemólisis en el recién nacido." ] "en" => array:1 [ "resumen" => "OjectiveABO-hemolytic disease occurs when ABO-incompatibility is present between the mother and the fetus. The objective of the present study was to analyze ABO-hemolytic disease in our center, to compare two serologic tests to diagnose it and to evaluate hematocrit values in cord blood samples. MethodsABO and Rh(D) group was found in 881 blood samples. Umbilical cord blood samples were obtained and direct antiglobulin test (DAT), eluate and microhematocrit were performed. ResultsABO-incompatibility was found in 134 (15.3%) cases. DAT was positive in 31.6% of patients studied with ABOincompatibility and the eluate identified the antibody in 68.4% of studied patients (p = 0.001). Hematocrit values were lower in the group with ABO-incompatibility than in the ABO-compatible group (44 ± 7.8% vs. 46.5 ± 6.5%; p = 0.029). ConclusionsAlthough severe ABO hemolytic disease is rare, ABO incompatibility is detected with current serologic tests and it causes a certain degree of hemolysis in newborn infants." ] ] "bibliografia" => array:2 [ "titulo" => "Bibliografía" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:15 [ 0 => array:3 [ "identificador" => "bib0005" "etiqueta" => "1." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:1 [ "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "V. Vengelen-Tyler" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "LibroEditado" => array:3 [ "titulo" => "Technical manual" "edicion" => "12.a" "serieFecha" => "1996" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0010" "etiqueta" => "2." 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Anales de Pediatría

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In Memoriam: Alfredo García-Alix (1956-2024)

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La enfermedad hemolítica ABO puede manifestarse cuando existe incompatibilidad de grupo ABO entre la madre y el hijo. El objetivo del presente estudio fue analizar la incompatibilidad serológica ABO presente en nuestro centro, comparar 2 técnicas immunohematológicas para su diagnóstico serológico y evaluar la repercusión de la incompatibilidad ABO en el hematócrito de la sangre de cordón umbilical.

Métodos

Se realizó el grupo ABO y Rh(D) en 881 muestras de sangre materna y de sangre de cordón. Con esta última se realizó la prueba de la antiglobulina directa (PAD), el eluido y el microhematócrito.

Resultados

La incompatibilidad ABO se dio en 134 (15,3%) casos. Ésta se detectó con la PAD y el eluido en un 31,6 y 68,4% de los casos estudiados, respectivamente (p = 0,001). El grupo con incompatibilidad ABO presentó unas cifras de hematócrito inferiores al grupo sin incompatibilidad, con una diferencia estadísticamente significativa (44 ± 7,8% frente a 46,5 ± 6,5%; p = 0,029).

Conclusiones

Aunque la enfermedad hemolítica ABO clínicamente significativa es rara, la incompatibilidad ABO se detecta con las técnicas serológicas comúnmente utilizadas y es causa de cierto grado de hemólisis en el recién nacido.

Palabras clave:

Enfermedad hemolítica ABO

Eluido

Prueba de la antiglobulina directa

Hematócrito

Ojective

ABO-hemolytic disease occurs when ABO-incompatibility is present between the mother and the fetus. The objective of the present study was to analyze ABO-hemolytic disease in our center, to compare two serologic tests to diagnose it and to evaluate hematocrit values in cord blood samples.

Methods

ABO and Rh(D) group was found in 881 blood samples. Umbilical cord blood samples were obtained and direct antiglobulin test (DAT), eluate and microhematocrit were performed.

Results

ABO-incompatibility was found in 134 (15.3%) cases. DAT was positive in 31.6% of patients studied with ABOincompatibility and the eluate identified the antibody in 68.4% of studied patients (p = 0.001). Hematocrit values were lower in the group with ABO-incompatibility than in the ABO-compatible group (44 ± 7.8% vs. 46.5 ± 6.5%; p = 0.029).

Conclusions

Although severe ABO hemolytic disease is rare, ABO incompatibility is detected with current serologic tests and it causes a certain degree of hemolysis in newborn infants.

Keywords:

ABO hemolytic disease

Eluate

Direct antiglobulin test

Hematocrit

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