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Vol. 61. Núm. 1.
Páginas 16-22 (julio 2004)
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Vol. 61. Núm. 1.
Páginas 16-22 (julio 2004)
Acceso a texto completo
Peroxidación lipídica y concentración de vitamina E plasmática en niños hipercolesterolémicos de Medellín (Colombia)
Lipid peroxidation and plasma vitamin e concentration in hypercholesterolemic children from medellin (Colombia)
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11595
C.M. Velásquez Rodrígueza,
Autor para correspondencia
claver@pijaos.udea.edu.co

Correspondencia: Calle 75, n.° 65–87, bloque 44. Oficina 107. Ciudadela Robledo. Universidad de Antioquia. Medellín, Colombia
, R.M. Uscátegui Peñuelaa, L.C. Burgos Herrerab
a Centro de Investigación en Alimentación y Nutrición. Escuela de Nutrición y Dietética. Universidad de Antioquia
b Departamento de Bioquímica y Fisiología. Facultad de Medicina. Universidad de Antioquia. Colombia
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Estadísticas
Antecedentes

Las alteraciones de los lípidos séricos en edades tempranas son factores de riesgo para desarrollar aterosclerosis; proceso en el que se cree que desempeña un papel importante la peroxidación lipídica junto con la hipercolesterolemia

Objetivo

Comparar entre dos grupos de niños, unos con niveles de lipoproteínas de baja densidad (LDL) igual o superior a 130mg/dl (hipercolesterolémicos) y otros con LDL inferior a 130mg/dl (control), las concentraciones plasmáticas de malondialdehído y vitamina E

Métodos

Estudio cruzado. Se incluyeron en el estudio escolares sanos de los colegios de la ciudad de Medellín seleccionados por personal de la Universidad de Antioquia basándose en una muestra probabilística

Sujetos

134 niños entre 6 y 9 años de edad, distribuidos en dos grupos: uno con 67 niños con hipercolesterolemia (LDL ≥ 130mg/dl) y otro control con 67 niños normocolesterolémicos (LDL < 130mg/dl)

Resultados

Los niños hipercolesterolémicos con una concentración sérica promedio de LDL de 147,7 ± 15,5mg/dl y un colesterol total de 212 ± 17mg/dl tenían mayores concentraciones plasmáticas de malondialdehído que el grupo control (0,18 ± 0,1 μM; 0,08 ± 0,06 μM, respectivamente; p < 0,0001). En el grupo de niños hipercolesterolémicos se encontró correlación negativa, entre la relación alfatocoferol/lípidos totales y la concentración de malondialdehído (r –0,337, p < 0,01)

Conclusión

La peroxidación lipídica fue mayor en niños hipercolesterolémicos y ésta aumentó a medida que disminuía la relación plasmática de alfatocoferol/lípidos totales (mg/g). La hipercolesterolemia y la peroxidación lipídica son factores de riesgo cardiovascular que coexisten y se instauran desde la edad escolar

Palabras clave:
Peroxidación lipídica
Alfatocoferol
LDL Hipercolesterolemia
Niños
Malondialdehído
Background

Serum lipid abnormalities in early childhood are risk factors for atherosclerosis. Lipid peroxidation might play an important role in this process, along with hypercholesterolemia

Objective

To compare plasma concentrations of malondialdehyde (MDA) and vitamin E in two groups of children: one group with low density lipoproteins (LDL) concentrations ≥ 130mg/dL and another group with LDL concentrations < 130mg/dL

Methods

Cross-sectional study

Healthy schoolchildren from the city of Medellin selected by probabilistic sampling performed by staff at the Universidad de Antioquia in Colombia

Sample

There were 134 subjects, aged 6 to 9 years old, divided into two groups: a group of 67 hypercholesterolemic children (LDL ≥ 130mg/dL) and a control group of 67 normocholesterolemic children (LDL < 130mg/dL)

Results

Hypercholesterolemic children with a mean serum LDL concentration of 147.7 ± 15.5mg/dL and a total cholesterol concentration of 212 ± 17mg/dL had higher MDA plasma concentrations than the control group (0.18 ± 0.1 ± μM; 0.08 ± 0.06 μM, p < 0,0001, respectively). A negative correlation was observed between -tocopherol/total lipids ratio and MDA values in children with hypercholesterolemia (r=–0.337 p < 0.01)

Conclusion

Lipid peroxidation was higher in hypercholesterolemic children than in the control group and was even higher when there was a deficit in the -tocopherol/total lipids ratio. Hypercholesterolemia and lipid peroxidation are co-existing risk factors that are established during school age

Key words:
Lipid peroxidation
α -tocopherol
LDL
Hypercholesterolemia
Children
Malondialdehyde
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Bibliografía
[1.]
K.B. Beckman, B.N. Ames.
The free radical theory of aging matures.
Physiol Rev, 78 (1998), pp. 547-581
[2.]
R.J. Wanders, P. Vreken, S. Ferdinandusse, G.A. Jansen, H.R. Waterham, C.W. Van Roermund, et al.
Peroxisomal fatty acid alpha- and beta-oxidation in humans: enzymology, peroxisomal metabolite transporters and peroxisomal diseases.
Biochem Soc Trans, 29 (2001), pp. 250-267
[3.]
D.C. Schwenke.
Antioxidants and Atherogenesis.
J Nutr Biochem, 9 (1998), pp. 424-445
[4.]
A.A. Quyyumi.
Endothelial function in health and disease: New insights into the genesis of cardiovascular disease.
Am J Med, 105 (1998), pp. 32S-39S
[5.]
G.S. Berenson, S.R. Srinivasan, W. Bao, W.P. Newman 3rd, R.E. Tracy, W.A. Wattigney.
Association between multiple cardiovascular risk factors and atherosclerosis in children and young adults. The Bogalusa Heart Study.
N Engl J Med, 338 (1998), pp. 1650-1656
[6.]
M.R. McCall, B. Frei.
Can antioxidant vitamins materially reduce oxidative damage in humans.
Free Radic Biol Med, 26 (1999), pp. 1034-1053
[7.]
R. Stocker.
The ambivalence of vitamin E in atherogenesis.
Trends Biochem Sci, 24 (1999), pp. 219-223
[8.]
Colombia. Ministerio de Salud, OPS, OMS. Situación de Salud de Colombia. Indicadores Básicos 2001. 1–15. Disponible en: www.minasulud.gov.co. Consultado en marzo de 2002.
[9.]
R.M. Uscátegui Peñuela, M.C. Álvarez Uribe, I. Laguado Salinas, W. Soler Terranova, L. Martínez Maluendas, R. Arias Arteaga, et al.
Cardiovascular risk factors in children and teenagers aged 6–18 years old from Medellin (Colombia).
An Pediatr, 58 (2003), pp. 411-417
[10.]
S. Chirico.
High-performance liquid chromatography-Based thiobarbituric acid test.
Methods Enzymol, 233 (1994), pp. 314-318
[11.]
NCEP. Report of the expert panel on blood cholesterol levels in children and adolescents.
Pediatrics, 89 (1992), pp. 531-537
[12.]
M. Burstein, H.R. Scholnick, R. Morfin.
Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions.
J Lipid Res, 11 (1970), pp. 583-595
[13.]
U. Nagele, O.E. Hageie, G. Sauer, G. Sauer, E. Wiedemann, P. Lehmann, A.W. Wahlefeid, W. Gruber.
Reagent for the enzymatic determination of serum total triglycerides with improved lipolytic efficiency.
J Clin Chem Clin Biochem, 22 (1984), pp. 165-174
[14.]
E. Gennaio.
Cholesterol reference method laboratory network. Boehringer Mannheim System.
(1997),
[15.]
W.T. Fridewald, R.Y. Levin, D.S. Fredrickson.
Estimations of the concentration of c-LDL in plasma without use of the preparative ultracentrifuge.
Clin Chem, 18 (1972), pp. 499-507
[16.]
D. Hoehler, A.A. Frohlich, R.R. Marquardt, H. Stelsovsky.
Extraction of alfa tocopherol from serum prior reversed-phase liquid chromatogaraphy.
J Agric Food Chem, 46 (1998), pp. 973-978
[17.]
National Center for Health Statistics N. Growth curves for children birth-18 years. U.S. Department of Health, Education and Welfare.
(1977),
[18.]
A. Must, G.E. Dallal, W.H. Dietz.
Reference data for obesity: 85th and 95th percentiles of body mass index (wt/ht2) and triceps skinfold thickness.
Am J Clin Nutr, 53 (1991), pp. 839-846
[19.]
S.J. Crossley.
Joint FAO/WHO Geneva consultation–acute dietary intake methodology.
Food Addit Contam, 17 (2000), pp. 557-562
[20.]
J. Groff, E. Vitamin.
Advanced nutrition and human metabolim, pp. 306-312
[21.]
F. Nielsen, B.B. Mikkelsen, J.B. Nielsen, H.R. Andersen, P. Grandjean, P. Lehmann.
Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors.
Clin Chem, 43 (1997), pp. 1209-1214
[22.]
C. Bonithon-Kopp, C. Coudray, C. Berr, P.J. Touboul, J.M. Feve, A. Favier, et al.
Combined effects of lipid peroxidation and antioxidant status on carotid atherosclerosis in a population aged 59–71 y: The EVA Study. Etude sur le Vieillisement Arteriel.
Am J Clin Nutr, 65 (1997), pp. 121-127
[23.]
D. Konukoglu, T. Akcay, T. Erdem.
Susceptibility of erythrocyte lipids to oxidation and erythrocyte antioxidant status in myocardial infarction.
Clin Biochem, 31 (1998), pp. 667-671
[24.]
Y. Dincer, T. Akcay, D. Konukoglu, H. Hatemi.
Erythrocyte susceptibility to lipid peroxidation in patients with coronary atherosclerosis.
Acta Med Okayama, 53 (1999), pp. 259-264
[25.]
M.M. Kesavulu, B.K. Rao, R. Giri, J. Vijaya, G. Subramanyam, C. Apparao.
Lipid peroxidation and antioxidant enzyme status in Type 2 diabetics with coronary heart disease.
Diabetes Res Clin Pract, 53 (2001), pp. 33-39
[26.]
E.I. Saygili, D. Konukoglu, C. Papila, T. Akcay.
Levels of plasma vitamin E, vitamin C, TBARS, and cholesterol in male patients with colorectal tumors.
Biochemistry (Mosc), 68 (2003), pp. 325-328
[27.]
G. Davi, P. Alessandrini, A. Mezzetti, G. Minotti, T. Bucciarelli, F. Costantini, et al.
In vivo formation of 8-Epi-prostaglandin F2 alpha is increased in hypercholesterolemia.
Arterioscler Thromb Vasc Biol, 17 (1997), pp. 3230-3235
[28.]
M.P. Reilly, D. Pratico, N. Delanty.
Increased formation of distinct F2 isoprostanes in hypercholesterolemia.
Circulation, 98 (1998), pp. 2822-2828
[29.]
J.L. Cracowski, D. Ploin, J. Bessard, J.P. Baguet, F. Stanke-Labesque, J.M. Mallion, et al.
Formation of isoprostanes in children with type IIa hypercholesterolemia.
J Cardiovasc Pharmacol, 38 (2001), pp. 228-231
[30.]
M.F. Muldoon, S.B. Kritchevsky, R.W. Evans, V.E. Kagan.
Serum total antioxidant activity in relative hypo- and hypercholesterolemia.
Free Radic Res, 25 (1996), pp. 239-245
[31.]
F. Van Lente, R. Daher, J.A. Waletzky.
Vitamin E compared with other potential risk factor concentrations in patients with and without coronary artery disease: A case-matched study.
Eur J Clin Chem Clin Biochem, 32 (1994), pp. 583-587
[32.]
E. Simon, J.L. Paul, T. Soni, A. Simon, N. Moatti.
Plasma and erythrocyte vitamin E content in asymptomatic hypercholesterolemic subjects.
Clin Chem, 43 (1997), pp. 285-289
[33.]
D.C. Schwenke.
Antioxidants, dietary fat saturation, lipoprotein oxidation and atherogenesis.
Nutrition, 12 (1996), pp. 377-379
[34.]
K. Miwa, Y. Miyagi, A. Igawa, K. Nakagawa, H. Inoue.
Vitamin E deficiency in variant angina.
Circulation, 94 (1996), pp. 14-18
[35.]
D.A. Street, G.W. Comstock, R.M. Salkeld, W. Schuep, M.J. Klag.
Serum antioxidants and myocardial infarction.
Are low levels of carotenoids and alpha-tocopherol risk factors for myocardial infarction? Circulation, 90 (1994), pp. 1154-1161
[36.]
W.A. Pryor.
Vitamin E and heart disease: Basic science to clinical intervention trials.
Free Radic Biol Med, 28 (2000), pp. 141-164
[37.]
K.F. Gey, P. Puska, P. Jordan, U.K. Moser.
Inverse correlation between plasma vitamin E and mortality from ischemic heart disease in cross-cultural epidemiology.
Am J Clin Nutr, 53 (1991), pp. 326S-334S
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