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as serum levels of S100&#946; seem to change with age&#44;<a class="elsevierStyleCrossRefs" href="#bib0135"><span class="elsevierStyleSup">1&#44;3&#44;15</span></a> with higher levels found in the first three years of life&#46;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">16</span></a> Furthermore&#44; serum concentrations of S100&#946; vary depending on the method used for its determination&#46;<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">17</span></a> All these are barriers to establishing reference values for children&#44; which nevertheless are essential in order to be able to use this protein in different diagnostic and prognostic scenarios&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">Thus&#44; the aim of our study was to determine the serum concentrations of S100&#946; in healthy children from birth to 14 years of age in our setting &#40;Hospital Universitario Central de Asturias&#41;&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Patients and methods</span><p id="par0020" class="elsevierStylePara elsevierViewall">We conducted a descriptive prospective study at the Hospital Universitario Central de Asturias &#40;HUCA&#41; between June 2008 and May 2010&#44; following approval by the Regional Clinical Research Ethics Committee&#46; We obtained the signed informed consent of the parents or guardians of all children included in the study&#46;</p><p id="par0025" class="elsevierStylePara elsevierViewall">We collected blood samples from healthy children aged 0&#8211;14 years as part of the preoperative evaluation for minor surgeries&#46; In addition to these samples&#44; we included blood samples from children seen at the paediatric emergency department that were collected for a variety of processes&#46; We excluded samples obtained from children with diseases that could lead to elevated S100&#946; serum concentrations&#44; such as acute or chronic diseases of the central nervous system &#40;CNS&#41;&#44; bone fractures&#44; Down syndrome&#44; kidney failure&#44; or developmental delay&#44; and samples from children that had experienced a febrile seizure in the week preceding the blood being drawn&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">We divided the sample into three age groups&#58; age less than 12 months&#44; age between 12 and 24 months&#44; and age more than 24 months&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">All samples were collected in bottles without an anticoagulant and submitted to the department of clinical biochemistry &#40;Medicine Laboratory&#41; of the HUCA&#46; They were centrifuged at 3000<span class="elsevierStyleHsp" style=""></span>rpm for 10<span class="elsevierStyleHsp" style=""></span>min &#40;Labofuge 400R&#59; Heraeus&#44; Boadilla&#44; Spain&#41;&#46; The serum was then collected and stored at &#8722;80<span class="elsevierStyleHsp" style=""></span>&#176;C until testing&#46; The determination of S100&#946; protein in serum samples was performed by electrochemiluminescence using a Cobas e-601 analyser &#40;Roche Diagnostics&#44; Mannheim&#44; Germany&#41;&#44; which performs a sandwich protocol with two incubation periods and a total testing time of 18<span class="elsevierStyleHsp" style=""></span>min&#46; Each determination was performed twice&#44; following manufacturer&#39;s recommendations&#46; The lower detection limit for the method was 0&#46;005<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#44; and the variability coefficient less than 2&#37;&#46;</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Data analysis</span><p id="par0040" class="elsevierStylePara elsevierViewall">We performed the statistical analysis with the software SPSS version 18 &#40;SPSS Inc&#44; Chicago&#44; USA&#41;&#46; We have expressed the data as means<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>standard deviations &#40;SDs&#41;&#44; medians&#44; and 95&#37; confidence intervals &#40;CIs&#41;&#46; After checking the normality of the distributions by interpretation of Q&#8211;Q plots&#44; we analysed the differences between groups with Student&#39;s <span class="elsevierStyleItalic">t</span> test &#40;two groups&#41; and multifactorial ANOVA with the Tukey HSD post hoc test &#40;more than two groups&#41;&#46; We set the level of statistical significance at <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;05&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Results</span><p id="par0045" class="elsevierStylePara elsevierViewall">We included 257 patients in the study&#44; of whom 179 were male &#40;69&#46;7&#37;&#41; and 78 female &#40;30&#46;4&#37;&#41;&#46; More than half of the blood samples &#40;67&#46;3&#37;&#41; were obtained from preoperative evaluations&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">The mean age and standard deviation of the boys were 5&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>3&#46;75 years&#44; with a median of 4&#46;76 years&#46; In girls&#44; the mean age was 4&#46;61<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>4&#46;19 years and the median 3&#46;40 years&#46; We did not find statistically significant differences in age between the sexes &#40;Student&#39;s <span class="elsevierStyleItalic">t</span>&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;096&#41;&#44; and 26&#46;8&#37; of the children were aged less than 2 years&#46;</p><p id="par0055" class="elsevierStylePara elsevierViewall">The mean serum concentration of the S100&#946; protein in the overall sample was 0&#46;156 &#40;0&#46;140&#8211;0&#46;172&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#46; The means in each age group were 0&#46;350 &#40;0&#46;280&#8211;0&#46;421&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in children aged less than 12 months&#44; 0&#46;165 &#40;0&#46;139&#8211;0&#46;190&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in children aged 12&#8211;24 months&#44; and 0&#46;121 &#40;0&#46;109&#8211;0&#46;133&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in children aged more than 24 months&#46;</p><p id="par0060" class="elsevierStylePara elsevierViewall">When we considered the sex of the patients&#44; the concentration was significantly higher in girls &#40;0&#46;191 &#91;0&#46;153&#8211;0&#46;230&#93;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#41; than in boys &#40;0&#46;141 &#91;0&#46;126&#8211;1&#46;156&#93;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#41; &#40;Student&#39;s <span class="elsevierStyleItalic">t</span>&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;018&#41;&#46; The analysis by age group did not show significant differences between the sexes &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;137&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;690 and <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;129&#44; respectively&#41;&#46;</p><p id="par0065" class="elsevierStylePara elsevierViewall">The multifactorial ANOVA showed that the mean serum concentrations of S100&#946; differed significantly between age groups &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;001&#41;&#44; while a significant difference in mean concentrations was not found between the sexes &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;141&#41;&#46; There was a significant interaction effect between both factors &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;017&#41;&#44; which indicates that the observed protein concentration differences between age groups are not the same in both sexes&#46; The serum levels of S100&#946; were higher in females than in males &#40;although these differences were not statistically significant&#41;&#44; especially in the youngest age group&#46; As for the differences observed between age groups&#44; the post hoc analysis showed that the protein concentration was significantly higher in the group of individuals aged less than 12 months compared to the other two groups &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41;&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0070" class="elsevierStylePara elsevierViewall">We calculated percentiles and 95&#37; CIs for the three age groups &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; We were unable to calculate the 95&#37; CI for every percentile for the group aged 1&#8211;2 years due to the small sample size&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0075" class="elsevierStylePara elsevierViewall">The regression analysis showed an inverse correlation between age and serum concentrations of the protein&#44; which decreased as aged increased&#44; with the decrease being most pronounced in the early years of life&#46; The separate analysis of protein concentrations in patients aged 2 years and less showed a linear relationship between concentration and age&#44; and the regression model obtained was statistically significant &#40;ANOVA&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;001&#41; &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Discussion</span><p id="par0080" class="elsevierStylePara elsevierViewall">In recent years&#44; there has been a growing interest in the use of biomarkers in the management of different pathologies and biological processes&#8212;which requires their determination to be easy&#44; quick and economical&#8212;and in learning the normal concentration patterns of these proteins in the populations of interest and the concentration patterns in the pathologies in which they are meant to be applied&#46; When it comes to CNS pathologies&#44; biomarkers with an adequate sensitivity and specificity are not yet available&#46; In the paediatric field&#44; protein S100&#946; could be a potentially useful biomarker in different biological fluids&#44; such as peripheral blood&#44; cord blood&#44; urine&#44; cerebrospinal fluid&#44; etc&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">There are often ethical and legal obstacles to performing clinical research in children&#44; which limits the number of patients included in studies&#46; This frequently leads to the extrapolation of values found in adults to paediatric patients&#46; However&#44; the influence of age&#44; which is associated with different degrees of organ and system development and maturation&#44; periods of rapid growth&#44; and differences in hepatic and renal metabolism&#44; may preclude the possibility of making such extrapolations&#46;</p><p id="par0090" class="elsevierStylePara elsevierViewall">To our knowledge&#44; our study is the first conducted in Spain to analyse S100&#946; concentrations in healthy paediatric patients&#44; which is why we had a keen interest in obtaining data on the concentration patterns of this protein in our setting using the method available to us &#40;electrochemiluminescence&#41;&#46;</p><p id="par0095" class="elsevierStylePara elsevierViewall">Most of the samples we used were obtained from preoperative evaluations for minor surgical interventions&#44; to which we added samples from children with other conditions &#40;the largest number corresponded to children with fever without source&#41; and observed that this circumstance did not influence serum S100&#946; levels or the presence of bilirubin in blood&#44; which was consistent with what Kleindienst et al&#46; had reported in adults&#46;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">18</span></a></p><p id="par0100" class="elsevierStylePara elsevierViewall">The first studies in paediatric patients did not address the potential effects of age&#44; but observed that concentrations were higher than in adults&#46; The study by Gazzolo et al&#46; was the first to provide data on paediatric patients broken down by age group&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">2</span></a> The highest serum levels of S100&#946; have been reported in healthy infants&#44; decreasing in later ages&#46;<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">3&#44;19</span></a> Higher concentrations of S100&#946; in newborns with lower gestational ages have been found in other biological fluids&#46;<a class="elsevierStyleCrossRefs" href="#bib0230"><span class="elsevierStyleSup">20&#44;21</span></a> The variation in the serum concentration of this protein between the early months of life and later ages may be due to differences in the blood&#8211;brain barrier and brain circulation&#44; as well as to the function of the protein&#44; with evidence of a gradual decrease in the serum concentration of S100&#946;&#46; This reduction could reflect a reduced release of this trophic factor at more advanced stages of brain maturation&#46; Other evidence has described a possible increase in serum S100&#946; from age 7 years on&#44; which may be related to height growth and nerve elongation&#44;<a class="elsevierStyleCrossRefs" href="#bib0140"><span class="elsevierStyleSup">2&#44;15</span></a> a fact that we did not observe in our sample&#46; This hypothesis is supported by the presence of S100&#946; in peripheral nerves and by its stimulating action on neurite outgrowth&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">2</span></a></p><p id="par0105" class="elsevierStylePara elsevierViewall">Previous studies on serum S100&#946; concentrations in children have provided few data for the first years of life&#44; and it is common for the youngest age group to be the least represented<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">3</span></a> or for the age distribution of the children to not be reported&#44;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">1</span></a> with the exception of the study conducted by Bouvier&#46;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">16</span></a> This may be related to the difficulty of obtaining samples in this age group&#46; Thus&#44; we considered that including 69 children in this age range in the study would provide highly relevant data for elucidating concentration patterns of S100&#946; in this age group&#46;</p><p id="par0110" class="elsevierStylePara elsevierViewall">We also observed an inverse correlation between serum S100&#946; concentrations and age&#44; consistent with previous evidence&#44;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">2</span></a> with concentrations being significantly higher in the first 2 years of life and essentially starting to decrease from age 1 year until reaching a stable mean value for all subsequent age groups&#46;</p><p id="par0115" class="elsevierStylePara elsevierViewall">Different age intervals have been suggested for analysis&#44; and reference values for serum S100&#946; concentrations in children aged more and less than 3 years proposed in recent years&#44; with mean S100&#946; values similar to the mean for our sample&#46;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">9&#44;16&#44;22</span></a> The differences observed between the various studies that use electrochemiluminescence methods are probably due to differences in the age distributions of their samples&#46; Our findings suggest that serum S100&#946; concentrations are stable from age 2 years with levels similar to those described in adults&#44;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">9&#44;22&#44;23</span></a> which makes it possible to establish reference values for children aged more than 2 years&#44; in whom the mean serum concentration of S100&#946; is 0&#46;120<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L &#40;0&#46;083&#41; with a 50th percentile of 0&#46;100<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#46; In a study of a sample of 186 children&#44; Bouvier proposed calculating reference values for different age subgroups in children aged less than 3 years &#40;0&#8211;3 months&#44; 4&#8211;9 months&#44; 10&#8211;24 months&#44; 25&#8211;36 months&#41; and found no significant differences between the groups&#46;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">16</span></a> While it is possible that such differences in the serum concentrations of S100&#946; do exist in the early months of life&#44; we consider that these divisions are not too practical&#46; In our sample&#44; we identified two age subgroups in children younger than 2 years with significantly different values &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;001&#41;&#46; Thus&#44; we propose reference values for children aged less than 1 year&#44; children aged 1&#8211;2 years&#44; and children aged more than 2 years&#46; Since one of the potential clinical applications of the determination of protein S100&#946; levels would be its use in the management of children with traumatic brain injury&#44; the age groups we propose would fit those proposed for the management of this pathology&#46;<a class="elsevierStyleCrossRefs" href="#bib0250"><span class="elsevierStyleSup">24&#44;25</span></a></p><p id="par0120" class="elsevierStylePara elsevierViewall">Gazzolo et al&#46; reported higher concentrations of S100&#946; in females&#44;<a class="elsevierStyleCrossRef" href="#bib0235"><span class="elsevierStyleSup">21</span></a> which they attributed to sex differences in the pattern of cerebral maturation&#44; both in the intrauterine period and in adulthood&#44;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">2</span></a> while the potential interaction of sex hormones with S100&#946; cannot be ruled out&#46; However&#44; subsequent studies have not observed these differences in healthy children&#46;<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">3&#44;26</span></a> When we compared S100&#946; levels in males and females in our sample for each age group &#40;because variations in the sex distribution of different age groups could be a source of bias&#41;&#44; we found no significant differences in the serum concentrations of this protein&#46; However&#44; the serum S100&#946; levels seemed higher in females in the youngest age group&#46;</p><p id="par0125" class="elsevierStylePara elsevierViewall">We think it is worth noting that the reference values for S100&#946; protein serum concentrations described in this study are the first proposed for the paediatric population of Spain&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Limitations of the study</span><p id="par0130" class="elsevierStylePara elsevierViewall"><ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005"><span class="elsevierStyleLabel">1&#46;</span><p id="par0135" class="elsevierStylePara elsevierViewall">The study was conducted in a single centre&#44; so the data may not be representative of other paediatric populations&#46;</p></li><li class="elsevierStyleListItem" id="lsti0010"><span class="elsevierStyleLabel">2&#46;</span><p id="par0140" class="elsevierStylePara elsevierViewall">The study included few children aged less than 2 years&#46;</p></li></ul></p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Conclusions</span><p id="par0145" class="elsevierStylePara elsevierViewall">When we analysed serum S100&#946; values in a sample of healthy children&#44; we observed the following&#58;<ul class="elsevierStyleList" id="lis0010"><li class="elsevierStyleListItem" id="lsti0015"><span class="elsevierStyleLabel">1&#46;</span><p id="par0150" class="elsevierStylePara elsevierViewall">The concentration of S100&#946; remains stable after age 2 years&#44; so that reference values for S100&#946; can be established for individuals aged more than 2 years&#46;</p></li><li class="elsevierStyleListItem" id="lsti0020"><span class="elsevierStyleLabel">2&#46;</span><p id="par0155" class="elsevierStylePara elsevierViewall">In the first two years of life&#44; the serum concentration of S100&#946; is higher&#44; the younger the child&#46;</p></li><li class="elsevierStyleListItem" id="lsti0025"><span class="elsevierStyleLabel">3&#46;</span><p id="par0160" class="elsevierStylePara elsevierViewall">There were no differences in the serum levels of S100&#946; between the sexes&#46;</p></li></ul></p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Funding</span><p id="par0165" class="elsevierStylePara elsevierViewall">XI Grants for Clinical Research and Epidemiology in Paediatrics of the Fundaci&#243;n Ernesto S&#225;nchez-Villares &#40;2008&#41;&#46; Funds received&#58; 2500 euro&#46;</p></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Conflicts of interest</span><p id="par0170" class="elsevierStylePara elsevierViewall">The authors have no conflicts of interest to declare</p></span></span>"
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        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">S100&#946; protein has been proposed as a potential biomarker for both chronic and acute neurological disorders&#46; Reference values of this protein are well defined in adults but not in children&#44; in whom serum levels appear to vary with age&#46; Reference values for serum S100&#946; in children from 0 to 14 years are presented&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Materials and methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">A prospective study was conducted on 257 healthy children&#44; who were divided into three age groups &#40;under 12 months&#44; 12&#8211;24 months and over 24 months&#41;&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The study included 179 boys and 78 girls&#44; with a mean age of 5&#46;5 &#40;3&#46;75&#41; years&#46; The mean serum concentration of protein S100&#946; was 0&#46;156 &#40;0&#46;140&#8211;0&#46;172&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#46; In children under 12 months&#44; serum S100&#946; concentration was 0&#46;350 &#40;0&#46;280&#8211;0&#46;421&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#59; 0&#46;165 &#40;0&#46;139&#8211;0&#46;190&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in the group between 12 and 24 months and 0&#46;121 &#40;0&#46;109&#8211;0&#46;133&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in children older than 24 months&#46; An inverse relationship was observed between age and serum S100&#946;&#44; which declines as age increases&#46; No differences were observed between sexes&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">The concentration of S100&#946; remains stable after two years of age&#44; being possible to establish a baseline of S100&#946; for over two years&#46; During the first two years of life&#44; S100&#946; serum concentration is higher&#44; the lower the age of the child&#46; No differences in serum S100&#946; levels between sexes are observed&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0005"
            "titulo" => "Introduction"
          ]
          1 => array:2 [
            "identificador" => "abst0010"
            "titulo" => "Materials and methods"
          ]
          2 => array:2 [
            "identificador" => "abst0015"
            "titulo" => "Results"
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          3 => array:2 [
            "identificador" => "abst0020"
            "titulo" => "Conclusions"
          ]
        ]
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      "es" => array:3 [
        "titulo" => "Resumen"
        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducci&#243;n</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">La prote&#237;na S100&#946; se ha propuesto como posible biomarcador en patolog&#237;a neurol&#243;gica&#44; tanto cr&#243;nica como aguda&#46; Los valores normales de esta prote&#237;na est&#225;n bien definidos en adultos&#44; no as&#237; en ni&#241;os&#44; en los que los valores s&#233;ricos parecen variar con la edad&#46; Nuestro objetivo es describir valores de referencia de S100&#946; s&#233;rica en ni&#241;os de 0 a 14 a&#241;os&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Material y m&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Estudio prospectivo en 257 ni&#241;os sanos&#46; Se establecieron 3 grupos por edad &#40;menores de 12 meses&#44; de 12 a 24 meses y mayores de 24 meses&#41;&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Se incluy&#243; a 179 ni&#241;os y 78 ni&#241;as&#46; La edad media &#177; DE fue de 5&#44;5 &#177; 3&#44;75 a&#241;os&#46; La concentraci&#243;n s&#233;rica media de la prote&#237;na S100&#946; en todo el grupo fue 0&#44;156 &#40;0&#44;140-0&#44;172&#41; &#956;g&#47;l&#46; En los menores de 12 meses&#44; la concentraci&#243;n s&#233;rica de S100&#946; fue de 0&#44;350 &#40;0&#44;280-0&#44;421&#41; &#956;g&#47;l&#59; 0&#44;165 &#40;0&#44;139-0&#44;190&#41; &#956;g&#47;l en el grupo entre 12 y 24 meses y 0&#44;121 &#40;0&#44;109-0&#44;133&#41; &#956;g&#47;l en el grupo de ni&#241;os mayores de 24 meses&#46; Se observ&#243; una relaci&#243;n inversa entre la edad y la concentraci&#243;n s&#233;rica de S100&#946;&#44; que desciende conforme se incrementa la edad&#46; No se observaron diferencias en cuanto al sexo&#46;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">La concentraci&#243;n de S100&#946; permanece estable a partir de los 2 a&#241;os de edad&#44; siendo posible establecer unos valores de referencia de S100&#946; para mayores de 2 a&#241;os&#46; En los 2 primeros a&#241;os de vida&#44; la concentraci&#243;n de S100&#946; s&#233;rica es m&#225;s elevada cuanto menor es la edad del ni&#241;o&#46; No se observan diferencias en el valor de S100&#946; s&#233;rica entre ambos sexos&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0025"
            "titulo" => "Introducci&#243;n"
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            "identificador" => "abst0030"
            "titulo" => "Material y m&#233;todos"
          ]
          2 => array:2 [
            "identificador" => "abst0035"
            "titulo" => "Resultados"
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          3 => array:2 [
            "identificador" => "abst0040"
            "titulo" => "Conclusiones"
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    "NotaPie" => array:1 [
      0 => array:2 [
        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Please cite this article as&#58; Arroyo Hern&#225;ndez M&#44; Rodr&#237;guez Su&#225;rez J&#44; &#193;lvarez Men&#233;ndez F&#46; Valores de referencia de la prote&#237;na S100&#946; en poblaci&#243;n pedi&#225;trica&#46; An Pediatr &#40;Barc&#41;&#46; 2016&#59;84&#58;254&#8211;259&#46;</p>"
      ]
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Serum S100&#946; protein levels by age&#46; The results are also disaggregated by sex &#40;dark grey for males and light grey for females&#41;&#46; The age groups between which significant differences were found are represented by the letters &#8216;a&#8217; &#40;&#60;12 months&#41; and &#8216;b&#8217; &#40;12&#8211;24 months and &#62;24 months&#41;&#46;</p>"
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          "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">&#40;A&#41; Regression analysis&#58; logarithmic curve fit for the entire dataset&#46; The figure shows the goodness of fit of the model &#40;<span class="elsevierStyleItalic">R</span><span class="elsevierStyleSup">2</span>&#41;&#46; &#40;B&#41; Linear regression model for the data obtained from patients aged up to 2 years&#46; The figure shows the goodness of fit &#40;<span class="elsevierStyleItalic">R</span><span class="elsevierStyleSup">2</span> and <span class="elsevierStyleItalic">R</span>&#41; and the linear regression equation&#46;</p>"
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                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td-with-role" title="table-head ; entry_with_role_rowhead " align="left" valign="top" scope="col">Percentile&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " colspan="3" align="center" valign="top" scope="col" style="border-bottom: 2px solid black">S100&#946; concentration in &#956;g&#47;L &#40;95&#37; CI&#41;</th></tr><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">&#60;12 months &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>33&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">12&#8211;24 months<br>&#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>36&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">&#62;24 months &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>188&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">2&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;076&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;030&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;043 &#40;0&#46;027&#8211;0&#46;048&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;119&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;043&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;048 &#40;0&#46;041&#8211;0&#46;051&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;145&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;066 &#40;0&#46;025&#8211;0&#46;111&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;053 &#40;0&#46;049&#8211;0&#46;060&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">25&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;187 &#40;0&#46;148&#8211;0&#46;244&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;113 &#40;0&#46;087&#8211;0&#46;138&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;071 &#40;0&#46;064&#8211;0&#46;078&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">50&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;296 &#40;0&#46;209&#8211;0&#46;428&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">75&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;483 &#40;0&#46;383&#8211;0&#46;630&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">90&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;645&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">95&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;734&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;328&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;246 &#40;0&#46;228&#8211;0&#46;456&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">97&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;830&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;331&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;410 &#40;0&#46;249&#8211;0&#46;520&#41;&nbsp;\t\t\t\t\t\t\n
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        "descripcion" => array:1 [
          "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Serum S100&#946; concentrations in the three age groups considered in the study&#44; expressed as mean and 95&#37; confidence interval &#40;CI&#41;&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:26 [
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              "identificador" => "bib0135"
              "etiqueta" => "1"
              "referencia" => array:1 [
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                  "contribucion" => array:1 [
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                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
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                        ]
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                  "host" => array:1 [
                    0 => array:2 [
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                      "titulo" => "Pediatric concentrations of S100B protein in blood&#58; age- and sex-related changes"
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                          "etal" => true
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                            1 => "F&#46; Michetti"
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                  "host" => array:1 [
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                      "Revista" => array:6 [
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                0 => array:2 [
                  "contribucion" => array:1 [
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                      "titulo" => "The serum S100B concentration is age dependent"
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                        0 => array:2 [
                          "etal" => true
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              "referencia" => array:1 [
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                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "S100&#58; a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
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                            0 => "R&#46; Donato"
                          ]
                        ]
                      ]
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Original Article
Serum S100β protein reference values in a paediatric population
Valores de referencia de la proteína S100β en población pediátrica
M. Arroyo Hernándeza,
Corresponding author
mireiaah@yahoo.es

Corresponding author.
, J. Rodríguez Suárezb, F. Álvarez Menéndezc
a Servicio de Pediatría, Hospital San Agustín, Avilés, Asturias, Spain
b Área de Gestión Clínica de Pediatría, Hospital Universitario Central de Asturias, Oviedo, Asturias, Spain
c Servicio de Bioquímica Clínica, Área de Gestión Clínica Laboratorio de Medicina, Hospital Universitario Central de Asturias, Oviedo, Asturias, Spain
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        "autoresLista" => "M&#46; Arroyo Hern&#225;ndez, J&#46; Rodr&#237;guez Su&#225;rez, F&#46; &#193;lvarez Men&#233;ndez"
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            "entidad" => "&#193;rea de Gesti&#243;n Cl&#237;nica de Pediatr&#237;a&#44; Hospital Universitario Central de Asturias&#44; Oviedo&#44; Asturias&#44; Spain"
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        "titulo" => "Valores de referencia de la prote&#237;na S100&#946; en poblaci&#243;n pedi&#225;trica"
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Serum S100&#946; protein levels by age&#46; The results are also disaggregated by sex &#40;dark grey for males and light grey for females&#41;&#46; The age groups between which significant differences were found are represented by the letters &#8216;a&#8217; &#40;&#60;12 months&#41; and &#8216;b&#8217; &#40;12&#8211;24 months and &#62;24 months&#41;&#46;</p>"
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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">The use of biomarkers has proven helpful in the management of different diseases&#46; Data for the paediatric population are usually scarce due to the restrictions placed on research studies&#46; Thus&#44; the concentration patterns of different markers&#44; initially studied in adults&#44; are usually extrapolated to children&#44; as has been the case of protein S100&#946;&#46; This protein&#44; which is predominantly found in astroglial and Schwann cells&#44;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">1</span></a> is secreted by astrocytes as a cytokine with neurotrophic and gliotrophic effects&#46;<a class="elsevierStyleCrossRefs" href="#bib0140"><span class="elsevierStyleSup">2&#8211;4</span></a> Under normal conditions&#44; it is detected in minimal concentrations in peripheral blood&#46; Its mean half-life ranges between 25<span class="elsevierStyleHsp" style=""></span>min and 2<span class="elsevierStyleHsp" style=""></span>h&#46;<a class="elsevierStyleCrossRefs" href="#bib0155"><span class="elsevierStyleSup">5&#8211;8</span></a> This protein may be of interest in cases of acute neurologic pathology&#44; such as brain injury or neonatal hypoxic-ischaemic encephalopathy&#46; Diagnostic strategies based on biomarkers have been proposed as an alternative to computed tomography in the management of brain injury&#46;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">9&#44;10</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">The serum concentrations of S100&#946; reported in healthy adults range between 0&#46;02 and 0&#46;05<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;dL&#46;<a class="elsevierStyleCrossRefs" href="#bib0185"><span class="elsevierStyleSup">11&#8211;14</span></a> These values cannot be extrapolated to children&#44; as serum levels of S100&#946; seem to change with age&#44;<a class="elsevierStyleCrossRefs" href="#bib0135"><span class="elsevierStyleSup">1&#44;3&#44;15</span></a> with higher levels found in the first three years of life&#46;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">16</span></a> Furthermore&#44; serum concentrations of S100&#946; vary depending on the method used for its determination&#46;<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">17</span></a> All these are barriers to establishing reference values for children&#44; which nevertheless are essential in order to be able to use this protein in different diagnostic and prognostic scenarios&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">Thus&#44; the aim of our study was to determine the serum concentrations of S100&#946; in healthy children from birth to 14 years of age in our setting &#40;Hospital Universitario Central de Asturias&#41;&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Patients and methods</span><p id="par0020" class="elsevierStylePara elsevierViewall">We conducted a descriptive prospective study at the Hospital Universitario Central de Asturias &#40;HUCA&#41; between June 2008 and May 2010&#44; following approval by the Regional Clinical Research Ethics Committee&#46; We obtained the signed informed consent of the parents or guardians of all children included in the study&#46;</p><p id="par0025" class="elsevierStylePara elsevierViewall">We collected blood samples from healthy children aged 0&#8211;14 years as part of the preoperative evaluation for minor surgeries&#46; In addition to these samples&#44; we included blood samples from children seen at the paediatric emergency department that were collected for a variety of processes&#46; We excluded samples obtained from children with diseases that could lead to elevated S100&#946; serum concentrations&#44; such as acute or chronic diseases of the central nervous system &#40;CNS&#41;&#44; bone fractures&#44; Down syndrome&#44; kidney failure&#44; or developmental delay&#44; and samples from children that had experienced a febrile seizure in the week preceding the blood being drawn&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">We divided the sample into three age groups&#58; age less than 12 months&#44; age between 12 and 24 months&#44; and age more than 24 months&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">All samples were collected in bottles without an anticoagulant and submitted to the department of clinical biochemistry &#40;Medicine Laboratory&#41; of the HUCA&#46; They were centrifuged at 3000<span class="elsevierStyleHsp" style=""></span>rpm for 10<span class="elsevierStyleHsp" style=""></span>min &#40;Labofuge 400R&#59; Heraeus&#44; Boadilla&#44; Spain&#41;&#46; The serum was then collected and stored at &#8722;80<span class="elsevierStyleHsp" style=""></span>&#176;C until testing&#46; The determination of S100&#946; protein in serum samples was performed by electrochemiluminescence using a Cobas e-601 analyser &#40;Roche Diagnostics&#44; Mannheim&#44; Germany&#41;&#44; which performs a sandwich protocol with two incubation periods and a total testing time of 18<span class="elsevierStyleHsp" style=""></span>min&#46; Each determination was performed twice&#44; following manufacturer&#39;s recommendations&#46; The lower detection limit for the method was 0&#46;005<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#44; and the variability coefficient less than 2&#37;&#46;</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Data analysis</span><p id="par0040" class="elsevierStylePara elsevierViewall">We performed the statistical analysis with the software SPSS version 18 &#40;SPSS Inc&#44; Chicago&#44; USA&#41;&#46; We have expressed the data as means<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>standard deviations &#40;SDs&#41;&#44; medians&#44; and 95&#37; confidence intervals &#40;CIs&#41;&#46; After checking the normality of the distributions by interpretation of Q&#8211;Q plots&#44; we analysed the differences between groups with Student&#39;s <span class="elsevierStyleItalic">t</span> test &#40;two groups&#41; and multifactorial ANOVA with the Tukey HSD post hoc test &#40;more than two groups&#41;&#46; We set the level of statistical significance at <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;05&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Results</span><p id="par0045" class="elsevierStylePara elsevierViewall">We included 257 patients in the study&#44; of whom 179 were male &#40;69&#46;7&#37;&#41; and 78 female &#40;30&#46;4&#37;&#41;&#46; More than half of the blood samples &#40;67&#46;3&#37;&#41; were obtained from preoperative evaluations&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">The mean age and standard deviation of the boys were 5&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>3&#46;75 years&#44; with a median of 4&#46;76 years&#46; In girls&#44; the mean age was 4&#46;61<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>4&#46;19 years and the median 3&#46;40 years&#46; We did not find statistically significant differences in age between the sexes &#40;Student&#39;s <span class="elsevierStyleItalic">t</span>&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;096&#41;&#44; and 26&#46;8&#37; of the children were aged less than 2 years&#46;</p><p id="par0055" class="elsevierStylePara elsevierViewall">The mean serum concentration of the S100&#946; protein in the overall sample was 0&#46;156 &#40;0&#46;140&#8211;0&#46;172&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#46; The means in each age group were 0&#46;350 &#40;0&#46;280&#8211;0&#46;421&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in children aged less than 12 months&#44; 0&#46;165 &#40;0&#46;139&#8211;0&#46;190&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in children aged 12&#8211;24 months&#44; and 0&#46;121 &#40;0&#46;109&#8211;0&#46;133&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in children aged more than 24 months&#46;</p><p id="par0060" class="elsevierStylePara elsevierViewall">When we considered the sex of the patients&#44; the concentration was significantly higher in girls &#40;0&#46;191 &#91;0&#46;153&#8211;0&#46;230&#93;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#41; than in boys &#40;0&#46;141 &#91;0&#46;126&#8211;1&#46;156&#93;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#41; &#40;Student&#39;s <span class="elsevierStyleItalic">t</span>&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;018&#41;&#46; The analysis by age group did not show significant differences between the sexes &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;137&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;690 and <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;129&#44; respectively&#41;&#46;</p><p id="par0065" class="elsevierStylePara elsevierViewall">The multifactorial ANOVA showed that the mean serum concentrations of S100&#946; differed significantly between age groups &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;001&#41;&#44; while a significant difference in mean concentrations was not found between the sexes &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;141&#41;&#46; There was a significant interaction effect between both factors &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;017&#41;&#44; which indicates that the observed protein concentration differences between age groups are not the same in both sexes&#46; The serum levels of S100&#946; were higher in females than in males &#40;although these differences were not statistically significant&#41;&#44; especially in the youngest age group&#46; As for the differences observed between age groups&#44; the post hoc analysis showed that the protein concentration was significantly higher in the group of individuals aged less than 12 months compared to the other two groups &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41;&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0070" class="elsevierStylePara elsevierViewall">We calculated percentiles and 95&#37; CIs for the three age groups &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; We were unable to calculate the 95&#37; CI for every percentile for the group aged 1&#8211;2 years due to the small sample size&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0075" class="elsevierStylePara elsevierViewall">The regression analysis showed an inverse correlation between age and serum concentrations of the protein&#44; which decreased as aged increased&#44; with the decrease being most pronounced in the early years of life&#46; The separate analysis of protein concentrations in patients aged 2 years and less showed a linear relationship between concentration and age&#44; and the regression model obtained was statistically significant &#40;ANOVA&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;001&#41; &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Discussion</span><p id="par0080" class="elsevierStylePara elsevierViewall">In recent years&#44; there has been a growing interest in the use of biomarkers in the management of different pathologies and biological processes&#8212;which requires their determination to be easy&#44; quick and economical&#8212;and in learning the normal concentration patterns of these proteins in the populations of interest and the concentration patterns in the pathologies in which they are meant to be applied&#46; When it comes to CNS pathologies&#44; biomarkers with an adequate sensitivity and specificity are not yet available&#46; In the paediatric field&#44; protein S100&#946; could be a potentially useful biomarker in different biological fluids&#44; such as peripheral blood&#44; cord blood&#44; urine&#44; cerebrospinal fluid&#44; etc&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">There are often ethical and legal obstacles to performing clinical research in children&#44; which limits the number of patients included in studies&#46; This frequently leads to the extrapolation of values found in adults to paediatric patients&#46; However&#44; the influence of age&#44; which is associated with different degrees of organ and system development and maturation&#44; periods of rapid growth&#44; and differences in hepatic and renal metabolism&#44; may preclude the possibility of making such extrapolations&#46;</p><p id="par0090" class="elsevierStylePara elsevierViewall">To our knowledge&#44; our study is the first conducted in Spain to analyse S100&#946; concentrations in healthy paediatric patients&#44; which is why we had a keen interest in obtaining data on the concentration patterns of this protein in our setting using the method available to us &#40;electrochemiluminescence&#41;&#46;</p><p id="par0095" class="elsevierStylePara elsevierViewall">Most of the samples we used were obtained from preoperative evaluations for minor surgical interventions&#44; to which we added samples from children with other conditions &#40;the largest number corresponded to children with fever without source&#41; and observed that this circumstance did not influence serum S100&#946; levels or the presence of bilirubin in blood&#44; which was consistent with what Kleindienst et al&#46; had reported in adults&#46;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">18</span></a></p><p id="par0100" class="elsevierStylePara elsevierViewall">The first studies in paediatric patients did not address the potential effects of age&#44; but observed that concentrations were higher than in adults&#46; The study by Gazzolo et al&#46; was the first to provide data on paediatric patients broken down by age group&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">2</span></a> The highest serum levels of S100&#946; have been reported in healthy infants&#44; decreasing in later ages&#46;<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">3&#44;19</span></a> Higher concentrations of S100&#946; in newborns with lower gestational ages have been found in other biological fluids&#46;<a class="elsevierStyleCrossRefs" href="#bib0230"><span class="elsevierStyleSup">20&#44;21</span></a> The variation in the serum concentration of this protein between the early months of life and later ages may be due to differences in the blood&#8211;brain barrier and brain circulation&#44; as well as to the function of the protein&#44; with evidence of a gradual decrease in the serum concentration of S100&#946;&#46; This reduction could reflect a reduced release of this trophic factor at more advanced stages of brain maturation&#46; Other evidence has described a possible increase in serum S100&#946; from age 7 years on&#44; which may be related to height growth and nerve elongation&#44;<a class="elsevierStyleCrossRefs" href="#bib0140"><span class="elsevierStyleSup">2&#44;15</span></a> a fact that we did not observe in our sample&#46; This hypothesis is supported by the presence of S100&#946; in peripheral nerves and by its stimulating action on neurite outgrowth&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">2</span></a></p><p id="par0105" class="elsevierStylePara elsevierViewall">Previous studies on serum S100&#946; concentrations in children have provided few data for the first years of life&#44; and it is common for the youngest age group to be the least represented<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">3</span></a> or for the age distribution of the children to not be reported&#44;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">1</span></a> with the exception of the study conducted by Bouvier&#46;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">16</span></a> This may be related to the difficulty of obtaining samples in this age group&#46; Thus&#44; we considered that including 69 children in this age range in the study would provide highly relevant data for elucidating concentration patterns of S100&#946; in this age group&#46;</p><p id="par0110" class="elsevierStylePara elsevierViewall">We also observed an inverse correlation between serum S100&#946; concentrations and age&#44; consistent with previous evidence&#44;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">2</span></a> with concentrations being significantly higher in the first 2 years of life and essentially starting to decrease from age 1 year until reaching a stable mean value for all subsequent age groups&#46;</p><p id="par0115" class="elsevierStylePara elsevierViewall">Different age intervals have been suggested for analysis&#44; and reference values for serum S100&#946; concentrations in children aged more and less than 3 years proposed in recent years&#44; with mean S100&#946; values similar to the mean for our sample&#46;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">9&#44;16&#44;22</span></a> The differences observed between the various studies that use electrochemiluminescence methods are probably due to differences in the age distributions of their samples&#46; Our findings suggest that serum S100&#946; concentrations are stable from age 2 years with levels similar to those described in adults&#44;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">9&#44;22&#44;23</span></a> which makes it possible to establish reference values for children aged more than 2 years&#44; in whom the mean serum concentration of S100&#946; is 0&#46;120<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L &#40;0&#46;083&#41; with a 50th percentile of 0&#46;100<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#46; In a study of a sample of 186 children&#44; Bouvier proposed calculating reference values for different age subgroups in children aged less than 3 years &#40;0&#8211;3 months&#44; 4&#8211;9 months&#44; 10&#8211;24 months&#44; 25&#8211;36 months&#41; and found no significant differences between the groups&#46;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">16</span></a> While it is possible that such differences in the serum concentrations of S100&#946; do exist in the early months of life&#44; we consider that these divisions are not too practical&#46; In our sample&#44; we identified two age subgroups in children younger than 2 years with significantly different values &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;001&#41;&#46; Thus&#44; we propose reference values for children aged less than 1 year&#44; children aged 1&#8211;2 years&#44; and children aged more than 2 years&#46; Since one of the potential clinical applications of the determination of protein S100&#946; levels would be its use in the management of children with traumatic brain injury&#44; the age groups we propose would fit those proposed for the management of this pathology&#46;<a class="elsevierStyleCrossRefs" href="#bib0250"><span class="elsevierStyleSup">24&#44;25</span></a></p><p id="par0120" class="elsevierStylePara elsevierViewall">Gazzolo et al&#46; reported higher concentrations of S100&#946; in females&#44;<a class="elsevierStyleCrossRef" href="#bib0235"><span class="elsevierStyleSup">21</span></a> which they attributed to sex differences in the pattern of cerebral maturation&#44; both in the intrauterine period and in adulthood&#44;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">2</span></a> while the potential interaction of sex hormones with S100&#946; cannot be ruled out&#46; However&#44; subsequent studies have not observed these differences in healthy children&#46;<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">3&#44;26</span></a> When we compared S100&#946; levels in males and females in our sample for each age group &#40;because variations in the sex distribution of different age groups could be a source of bias&#41;&#44; we found no significant differences in the serum concentrations of this protein&#46; However&#44; the serum S100&#946; levels seemed higher in females in the youngest age group&#46;</p><p id="par0125" class="elsevierStylePara elsevierViewall">We think it is worth noting that the reference values for S100&#946; protein serum concentrations described in this study are the first proposed for the paediatric population of Spain&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Limitations of the study</span><p id="par0130" class="elsevierStylePara elsevierViewall"><ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005"><span class="elsevierStyleLabel">1&#46;</span><p id="par0135" class="elsevierStylePara elsevierViewall">The study was conducted in a single centre&#44; so the data may not be representative of other paediatric populations&#46;</p></li><li class="elsevierStyleListItem" id="lsti0010"><span class="elsevierStyleLabel">2&#46;</span><p id="par0140" class="elsevierStylePara elsevierViewall">The study included few children aged less than 2 years&#46;</p></li></ul></p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Conclusions</span><p id="par0145" class="elsevierStylePara elsevierViewall">When we analysed serum S100&#946; values in a sample of healthy children&#44; we observed the following&#58;<ul class="elsevierStyleList" id="lis0010"><li class="elsevierStyleListItem" id="lsti0015"><span class="elsevierStyleLabel">1&#46;</span><p id="par0150" class="elsevierStylePara elsevierViewall">The concentration of S100&#946; remains stable after age 2 years&#44; so that reference values for S100&#946; can be established for individuals aged more than 2 years&#46;</p></li><li class="elsevierStyleListItem" id="lsti0020"><span class="elsevierStyleLabel">2&#46;</span><p id="par0155" class="elsevierStylePara elsevierViewall">In the first two years of life&#44; the serum concentration of S100&#946; is higher&#44; the younger the child&#46;</p></li><li class="elsevierStyleListItem" id="lsti0025"><span class="elsevierStyleLabel">3&#46;</span><p id="par0160" class="elsevierStylePara elsevierViewall">There were no differences in the serum levels of S100&#946; between the sexes&#46;</p></li></ul></p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Funding</span><p id="par0165" class="elsevierStylePara elsevierViewall">XI Grants for Clinical Research and Epidemiology in Paediatrics of the Fundaci&#243;n Ernesto S&#225;nchez-Villares &#40;2008&#41;&#46; Funds received&#58; 2500 euro&#46;</p></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Conflicts of interest</span><p id="par0170" class="elsevierStylePara elsevierViewall">The authors have no conflicts of interest to declare</p></span></span>"
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          "titulo" => "Keywords"
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          "titulo" => "Resumen"
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          "titulo" => "Funding"
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          "titulo" => "Conflicts of interest"
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    "tienePdf" => true
    "fechaRecibido" => "2015-04-01"
    "fechaAceptado" => "2015-07-15"
    "PalabrasClave" => array:2 [
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        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
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          "palabras" => array:3 [
            0 => "Protein S100&#946;"
            1 => "Reference values"
            2 => "Children"
          ]
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        0 => array:4 [
          "clase" => "keyword"
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          "palabras" => array:3 [
            0 => "Prote&#237;na S100&#946;"
            1 => "Valores de referencia"
            2 => "Ni&#241;os"
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    "resumen" => array:2 [
      "en" => array:3 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">S100&#946; protein has been proposed as a potential biomarker for both chronic and acute neurological disorders&#46; Reference values of this protein are well defined in adults but not in children&#44; in whom serum levels appear to vary with age&#46; Reference values for serum S100&#946; in children from 0 to 14 years are presented&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Materials and methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">A prospective study was conducted on 257 healthy children&#44; who were divided into three age groups &#40;under 12 months&#44; 12&#8211;24 months and over 24 months&#41;&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The study included 179 boys and 78 girls&#44; with a mean age of 5&#46;5 &#40;3&#46;75&#41; years&#46; The mean serum concentration of protein S100&#946; was 0&#46;156 &#40;0&#46;140&#8211;0&#46;172&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#46; In children under 12 months&#44; serum S100&#946; concentration was 0&#46;350 &#40;0&#46;280&#8211;0&#46;421&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L&#59; 0&#46;165 &#40;0&#46;139&#8211;0&#46;190&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in the group between 12 and 24 months and 0&#46;121 &#40;0&#46;109&#8211;0&#46;133&#41;<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;L in children older than 24 months&#46; An inverse relationship was observed between age and serum S100&#946;&#44; which declines as age increases&#46; No differences were observed between sexes&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">The concentration of S100&#946; remains stable after two years of age&#44; being possible to establish a baseline of S100&#946; for over two years&#46; During the first two years of life&#44; S100&#946; serum concentration is higher&#44; the lower the age of the child&#46; No differences in serum S100&#946; levels between sexes are observed&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0005"
            "titulo" => "Introduction"
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          1 => array:2 [
            "identificador" => "abst0010"
            "titulo" => "Materials and methods"
          ]
          2 => array:2 [
            "identificador" => "abst0015"
            "titulo" => "Results"
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        "titulo" => "Resumen"
        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducci&#243;n</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">La prote&#237;na S100&#946; se ha propuesto como posible biomarcador en patolog&#237;a neurol&#243;gica&#44; tanto cr&#243;nica como aguda&#46; Los valores normales de esta prote&#237;na est&#225;n bien definidos en adultos&#44; no as&#237; en ni&#241;os&#44; en los que los valores s&#233;ricos parecen variar con la edad&#46; Nuestro objetivo es describir valores de referencia de S100&#946; s&#233;rica en ni&#241;os de 0 a 14 a&#241;os&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Material y m&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Estudio prospectivo en 257 ni&#241;os sanos&#46; Se establecieron 3 grupos por edad &#40;menores de 12 meses&#44; de 12 a 24 meses y mayores de 24 meses&#41;&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Se incluy&#243; a 179 ni&#241;os y 78 ni&#241;as&#46; La edad media &#177; DE fue de 5&#44;5 &#177; 3&#44;75 a&#241;os&#46; La concentraci&#243;n s&#233;rica media de la prote&#237;na S100&#946; en todo el grupo fue 0&#44;156 &#40;0&#44;140-0&#44;172&#41; &#956;g&#47;l&#46; En los menores de 12 meses&#44; la concentraci&#243;n s&#233;rica de S100&#946; fue de 0&#44;350 &#40;0&#44;280-0&#44;421&#41; &#956;g&#47;l&#59; 0&#44;165 &#40;0&#44;139-0&#44;190&#41; &#956;g&#47;l en el grupo entre 12 y 24 meses y 0&#44;121 &#40;0&#44;109-0&#44;133&#41; &#956;g&#47;l en el grupo de ni&#241;os mayores de 24 meses&#46; Se observ&#243; una relaci&#243;n inversa entre la edad y la concentraci&#243;n s&#233;rica de S100&#946;&#44; que desciende conforme se incrementa la edad&#46; No se observaron diferencias en cuanto al sexo&#46;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">La concentraci&#243;n de S100&#946; permanece estable a partir de los 2 a&#241;os de edad&#44; siendo posible establecer unos valores de referencia de S100&#946; para mayores de 2 a&#241;os&#46; En los 2 primeros a&#241;os de vida&#44; la concentraci&#243;n de S100&#946; s&#233;rica es m&#225;s elevada cuanto menor es la edad del ni&#241;o&#46; No se observan diferencias en el valor de S100&#946; s&#233;rica entre ambos sexos&#46;</p></span>"
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        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Please cite this article as&#58; Arroyo Hern&#225;ndez M&#44; Rodr&#237;guez Su&#225;rez J&#44; &#193;lvarez Men&#233;ndez F&#46; Valores de referencia de la prote&#237;na S100&#946; en poblaci&#243;n pedi&#225;trica&#46; An Pediatr &#40;Barc&#41;&#46; 2016&#59;84&#58;254&#8211;259&#46;</p>"
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Serum S100&#946; protein levels by age&#46; The results are also disaggregated by sex &#40;dark grey for males and light grey for females&#41;&#46; The age groups between which significant differences were found are represented by the letters &#8216;a&#8217; &#40;&#60;12 months&#41; and &#8216;b&#8217; &#40;12&#8211;24 months and &#62;24 months&#41;&#46;</p>"
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          "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">&#40;A&#41; Regression analysis&#58; logarithmic curve fit for the entire dataset&#46; The figure shows the goodness of fit of the model &#40;<span class="elsevierStyleItalic">R</span><span class="elsevierStyleSup">2</span>&#41;&#46; &#40;B&#41; Linear regression model for the data obtained from patients aged up to 2 years&#46; The figure shows the goodness of fit &#40;<span class="elsevierStyleItalic">R</span><span class="elsevierStyleSup">2</span> and <span class="elsevierStyleItalic">R</span>&#41; and the linear regression equation&#46;</p>"
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                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td-with-role" title="table-head ; entry_with_role_rowhead " align="left" valign="top" scope="col">Percentile&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " colspan="3" align="center" valign="top" scope="col" style="border-bottom: 2px solid black">S100&#946; concentration in &#956;g&#47;L &#40;95&#37; CI&#41;</th></tr><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">&#60;12 months &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>33&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">12&#8211;24 months<br>&#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>36&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">&#62;24 months &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>188&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">2&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;076&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;030&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;043 &#40;0&#46;027&#8211;0&#46;048&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;119&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;043&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;048 &#40;0&#46;041&#8211;0&#46;051&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;145&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;066 &#40;0&#46;025&#8211;0&#46;111&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;053 &#40;0&#46;049&#8211;0&#46;060&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">25&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;187 &#40;0&#46;148&#8211;0&#46;244&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">75&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;483 &#40;0&#46;383&#8211;0&#46;630&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="char" valign="top">90&nbsp;\t\t\t\t\t\t\n
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        "descripcion" => array:1 [
          "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Serum S100&#946; concentrations in the three age groups considered in the study&#44; expressed as mean and 95&#37; confidence interval &#40;CI&#41;&#46;</p>"
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    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:26 [
            0 => array:3 [
              "identificador" => "bib0135"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "S-100beta protein-serum levels in healthy children and its association with outcome in pediatric traumatic brain injury"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "P&#46; Spinella"
                            1 => "T&#46; Dominguez"
                            2 => "H&#46; Drott"
                            3 => "J&#46; Huh"
                            4 => "L&#46; McCormick"
                            5 => "A&#46; Rajendra"
                          ]
                        ]
                      ]
                    ]
                  ]
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                    0 => array:2 [
                      "doi" => "10.1097/01.CCM.0000053644.16336.52"
                      "Revista" => array:7 [
                        "tituloSerie" => "Crit Care Med"
                        "fecha" => "2003"
                        "volumen" => "31"
                        "paginaInicial" => "939"
                        "paginaFinal" => "945"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12627009"
                            "web" => "Medline"
                          ]
                        ]
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                          "pii" => "S016164200900671X"
                          "estado" => "S300"
                          "issn" => "01616420"
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              "identificador" => "bib0140"
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        "titulo" => "Acknowledgments"
        "texto" => "<p id="par0175" class="elsevierStylePara elsevierViewall">We want to thank the <span class="elsevierStyleGrantSponsor" id="gs1">Fundaci&#243;n Ernesto S&#225;nchez Villares</span> for awarding us a grant for the amount of 2500 euro in the XI Convocatoria de Ayudas a la Investigaci&#243;n Cl&#237;nica y Epidemiol&#243;gica en Pediatr&#237;a &#40;2008&#41; for the project titled &#8220;Utilidad de la prote&#237;na S-100 beta en el estudio de los traumatismos craneoencef&#225;licos leves en una poblaci&#243;n pedi&#225;trica&#8221; &#91;Usefulness of S100&#946; protein in the study of mild traumatic brain injury in a paediatric population&#93;&#46;</p> <p id="par0180" class="elsevierStylePara elsevierViewall">We also thank Roche Diagnostics for their disinterested collaboration in this project&#46;</p>"
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Article information
ISSN: 23412879
Original language: English
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