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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Globally&#44; acute otitis media &#40;AOM&#41; is one of the most common childhood infections affecting over 75&#37; of children younger than 3 years of age<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">1</span></a> and representing the most common condition for antibiotic prescription in young children&#46;<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">2</span></a> Approximately 709 million cases of AOM occur throughout the world each year&#44; of which 51&#37; occur in children younger than 5 years of age&#46;<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">3</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">In Spain&#44; the annual incidence of AOM in children below 2 years of age is estimated at 392 per 1000 person-years&#44; as compared with 263 per 1000 person-years in children aged between 3 and 5 years&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">4</span></a> Antibiotics are prescribed as first-line treatment in more than 90&#37; of Spanish children presenting with symptoms of AOM &#40;e&#46;g&#46; ear discharge&#41;&#44; although an initial observation is recommended beforehand&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">5</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">Following the introduction of the 7-valent pneumococcal conjugate vaccine &#40;PCV&#41; &#40;PCV7&#59; <span class="elsevierStyleItalic">Prevenar 7</span>&#44; Pfizer&#47;Wyeth&#44; USA&#41; onto the Spanish private market in 2001&#44;<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">6</span></a> the vaccination coverage rate in children younger than 5 years had reached 50&#8211;60&#37; by 2007&#46;<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">7</span></a> Other licensed PCV formulations have since been introduced in Spain&#44; including the 10-valent PCV &#40;PCV10&#59; <span class="elsevierStyleItalic">Synflorix</span>&#8482;&#44; GSK Vaccines&#44; Belgium&#41; in 2009 and 13-valent PCV &#40;PCV13&#59; <span class="elsevierStyleItalic">Prevenar 13</span>&#44; Pfizer&#47;Wyeth&#44; USA&#41; in 2010&#46;<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">7</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">Before the introduction of PCV&#44; <span class="elsevierStyleItalic">Streptococcus pneumoniae</span> &#40;Spn&#41; and <span class="elsevierStyleItalic">Haemophilus influenzae</span> &#40;Hi&#41; were the most important etiological bacterial agents for AOM in Spanish children&#46;<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">8</span></a> However&#44; the underlying microbiology of infectious diseases changes over time as a consequence of vaccination and antibiotic consumption<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">9</span></a> and several global studies have documented a decrease in the circulation of pneumococcal vaccine serotypes and an increase in non-vaccine serotypes and Hi serotypes since PCV7 implementation&#46;<a class="elsevierStyleCrossRef" href="#bib0195"><span class="elsevierStyleSup">10</span></a> This study was designed to assess the bacterial etiology of AOM in Spanish children younger than 3 years of age using both culture and polymerase chain reaction &#40;PCR&#41;&#59; it complements a previous study describing the bacterial etiology of recurrent AOM and AOM treatment failures in Spanish children during the post-PCV era&#46;<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">7</span></a></p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Material and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Study design and participants</span><p id="par0025" class="elsevierStylePara elsevierViewall">This prospective&#44; epidemiological study was conducted at seven centres across Spain between February 2009 and May 2012 &#40;GSK study identifier&#58; 111425&#41;&#46; Children aged &#8805;3 to 36 months with ear&#8211;nose&#8211;throat specialist-confirmed AOM&#44; and who could provide a middle ear fluid sample &#40;MEF&#41;&#44; were enrolled in the study&#46; MEF samples were collected by tympanocentesis&#59; in case of otorrhea&#44; the samples were collected via deep aspiration of MEF through needle insertion after ear canal cleaning&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">AOM was confirmed based on the acute and sudden onset of the disease within the previous 3 days&#58; fever&#44; irritability&#44; intense erythema of the tympanum or earache&#59; and presence of effusion in the middle ear&#46; The presence of middle ear effusion was characterized by tympanum bulging&#44; limited or no mobility in the tympanum &#40;evaluated using pneumatic otoscopy&#41;&#44; fluid behind the tympanum and&#47;or otorrhea &#40;defined as perforation occurring within 48<span class="elsevierStyleHsp" style=""></span>h before enrollment&#41;&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">Children were excluded from participating in the study if they were hospitalized during the AOM episode &#40;to exclude possible confusion with intrahospital infections&#41; or in the event of otitis externa&#47;otitis media with effusion&#58; AOM for &#62;72<span class="elsevierStyleHsp" style=""></span>h or otorrhea for &#62;48<span class="elsevierStyleHsp" style=""></span>h before enrollment&#59; or in situ transtympanic aerators&#46; Children who received antibiotics for illnesses other than AOM during the 72-hour period before enrollment and were prescribed antibiotics before performing tympanocentesis were also excluded&#46; Finally&#44; patients with protocol-forbidden medical conditions were excluded&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">The study adhered to the principles of Good Clinical Practice&#44; including the 1964 Declaration of Helsinki and local Spanish rules and regulations&#46; The study was approved by the Institutional Review Board of each participating center and parents&#47;guardians provided written informed consent before enrollment&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Bacterial identification and antibacterial susceptibility testing</span><p id="par0045" class="elsevierStylePara elsevierViewall">MEF samples were collected by tympanocentesis performed under otoscopy through a reusable <span class="elsevierStyleItalic">CDT Aspirator</span> attached to a <span class="elsevierStyleItalic">CDT speculum</span>&#44; or by sampling of spontaneous otorrhea&#46; In cases of bilateral AOM&#44; MEF samples were collected from both ears&#46; Tympanocentesis was performed without anesthesia&#46; Aspirated MEF samples were inoculated onto Amies transport medium<a class="elsevierStyleCrossRef" href="#bib0200"><span class="elsevierStyleSup">11</span></a> and kept at room temperature for 16<span class="elsevierStyleHsp" style=""></span>h &#40;and up to a maximum of 48<span class="elsevierStyleHsp" style=""></span>h&#41; before subsequent analysis at a GSK-designated laboratory&#46; MEF samples were then inoculated onto chocolate &#40;with bacitracin for otorrhea samples&#41; and blood agar &#40;with nalidixic acid&#41;&#46; Bacterial identification was undertaken using standard bacteriological procedures&#46;<a class="elsevierStyleCrossRef" href="#bib0205"><span class="elsevierStyleSup">12</span></a> Serotyping for Spn was performed by Quellung&#39;s reaction and for Hi using monovalent anti-sera&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">Culture-negative samples were further analyzed using real-time PCR assay for the presence of the pneumococcal pneumolysin &#40;ply&#41; gene&#44;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">13</span></a> pneumococcal wzg gene&#44;<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">14</span></a> and the omp2 gene for non-capsulated and capsulated Hi&#46;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">15</span></a> Inhibited PCR reactions were all checked using a commercial exogenous internal positive control &#40;Applied Biosystems&#44; Foster City&#44; CA&#44; USA&#41;&#46; A subsequent extraction for eliminating inhibitors was performed on inhibited samples using 20&#37; &#40;w&#47;v&#41; Chelex-100 resin &#40;BioRad Laboratories&#44; Hercules&#44; CA&#44; USA&#41;&#46; The samples were further tested for antibacterial susceptibility&#46; Minimal inhibitory concentrations were derived using <span class="elsevierStyleItalic">Etest</span> &#40;bioM&#233;rieux&#44; France&#41; and interpreted with the criteria published by the Clinical and Laboratory Standards Institute in 2009&#46;<a class="elsevierStyleCrossRef" href="#bib0225"><span class="elsevierStyleSup">16</span></a></p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Statistical analyses</span><p id="par0055" class="elsevierStylePara elsevierViewall">Analyses were performed on children meeting all the selection criteria&#44; complying with protocol-defined procedures&#44; and from whom laboratory results of the MEF sample were available&#46; The proportion of AOM episodes caused by Spn&#44; Hi and other bacterial pathogens and the percentages of Spn and Hi serotypes were calculated&#46; Only the first AOM episode was included and any recurrent AOM cases were excluded from analyses&#46; The antibacterial susceptibility of Spn and Hi was determined&#46; History of previous PCV&#44; Hi-type B and influenza vaccinations was recorded&#46; All statistical analyses were performed using <span class="elsevierStyleItalic">SAS version 9&#46;2</span> &#40;SAS Institute Inc&#46;&#44; Cary&#44; NC&#44; USA&#41;&#46;</p></span></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Results</span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Study participants and demographics</span><p id="par0060" class="elsevierStylePara elsevierViewall">Of 125 AOM episodes in 124 subjects&#44; recorded during the study period&#44; 117 episodes &#40;tympanocentesis<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>91&#59; otorrhea<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>26&#41; &#40;from 117 subjects&#41; were analyzed&#46; Only one subject had two AOM episodes&#44; of which one episode was eliminated from the analyses&#46; Therefore&#44; a total of eight AOM episodes were excluded from the analyses&#58; protocol violation &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>1&#41;&#44; having a protocol-forbidden medical condition &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>4&#41; and missing MEF&#47;otorrhea sample &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#41;&#46; The median age of children was 17 months &#40;range&#58; 3&#8211;35&#41; and 60&#46;7&#37; were male&#46; AOM episodes were most frequently observed in children aged 11&#8211;22 months &#40;46&#46;2&#37;&#59; 54&#47;117&#41; followed by 23&#8211;35 months &#40;32&#46;5&#37;&#59; 38&#47;117&#41; and 3&#8211;10 months &#40;21&#46;4&#37;&#59; 25&#47;117&#41;&#46; At least one dose of a pneumococcal vaccine and Hi-type B vaccine was received by 69&#46;2&#37; and 95&#46;7&#37; subjects&#44; respectively&#46; None of the subjects received influenza vaccine&#46; <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a> presents information on age groups in the study&#44; summary of vaccination history and antibiotic treatment&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Bacterial identification</span><p id="par0065" class="elsevierStylePara elsevierViewall">The microbiological results are reported in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46; Bacterial culture tests were performed for 117 episodes&#46; Growth for any bacterium was cultured from 66&#37; &#40;77&#47;117&#41; of episodes&#58; 82&#37; of which &#40;63&#47;77&#41; were culture-positive for at least one bacterium under study &#40;Spn&#44; Hi&#44; <span class="elsevierStyleItalic">Streptococcus pyogenes</span> &#91;Spy&#93; or <span class="elsevierStyleItalic">Moraxella catarrhalis</span> &#91;Mcat&#93;&#41;&#46; The most frequent bacteria were Hi &#40;42&#37;&#59; 32&#47;77&#41; and Spn &#40;31&#37;&#59; 24&#47;77&#41;&#46; Spy was isolated in 10 samples and Mcat in 1 sample&#59; co-infection was detected in 10 episodes&#44; of which 4 co-infections involved bacterium under study&#58; Spn and Hi &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#41;&#59; Spy and Hi &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>1&#41;&#46; The remaining 40 episodes were culture-negative&#46; However&#44; PCR was performed for 42 episodes &#40;40 culture-negative episodes<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>2 culture-positive episodes&#41;&#44; of which 48&#37; &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>20&#41; were found to be Hi-positive and 55&#37; &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>23&#41; were Spn-positive&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0070" class="elsevierStylePara elsevierViewall">Combined culture and PCR results indicated that 52 AOM episodes &#40;44&#37;&#41; were Hi-positive and 46 &#40;39&#37;&#41; were Spn-positive&#59; 17 episodes &#40;15&#37;&#41; were positive for both Hi and Spn&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">The most common Spn serotypes amongst culture-positive episodes were 19F &#40;17&#37;&#59; 4&#47;24&#41; and 19A &#40;13&#37;&#59; 3&#47;24&#41; &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41;&#46; In episodes from children who had been previously vaccinated&#44; PCV7 vaccine serotypes &#40;14 and 19F&#41; were present in 19&#37; &#40;3&#47;16&#41; of episodes and PCV7&#47;PCV13 vaccine serotype &#40;6B&#41; was present in 6&#37; &#40;1&#47;16&#41; of episodes &#40;<a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>&#41;&#46; PCV7 vaccine serotype &#40;19F&#41; was present in 25&#37; &#40;2&#47;8&#41; episodes from unvaccinated children&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><elsevierMultimedia ident="tbl0015"></elsevierMultimedia><p id="par0080" class="elsevierStylePara elsevierViewall">All 32 Hi-positive episodes were non-typeable &#40;NTHi&#41;&#58; 69&#37; &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>22&#41; in vaccinated &#40;PCV vaccine&#41; and 31&#37; &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>10&#41; in unvaccinated &#40;PCV vaccine&#41; children&#46;</p></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Antibacterial susceptibility</span><p id="par0085" class="elsevierStylePara elsevierViewall">Of the 24 Spn-positive episodes&#44; all &#40;100&#37;&#41; were susceptible to cefotaxime&#44; 22 &#40;92&#37;&#41; to amoxicillin&#44; 17 &#40;71&#37;&#41; to erythromycin and 17 &#40;74&#37;&#41; to cefpodoxime &#40;since there was one missing Spn-positive episode susceptible to only cefpodoxime&#44; 23 of 24 Spn-positive episodes were considered for the analyses&#41;&#46; Ten episodes were susceptible and intermediately susceptible to penicillin G and four &#40;17&#37;&#41; were resistant to penicillin &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46; Serotype 19A was most commonly resistant to penicillin G&#44; amoxicillin&#44; cefpodoxime&#44; and erythromycin&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><p id="par0090" class="elsevierStylePara elsevierViewall">Of the 32 Hi-positive episodes&#44; all &#40;100&#37;&#41; were susceptible to both cefpodoxime and cefotaxime and 26 &#40;81&#37;&#41; to amoxicillin&#47;clavulanic acid&#46; Six episodes &#40;19&#37;&#41; were resistant to amoxicillin&#47;clavulanic acid &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>&#41;&#46;</p><elsevierMultimedia ident="fig0015"></elsevierMultimedia><p id="par0095" class="elsevierStylePara elsevierViewall">Multi-drug resistance was observed in 17&#37; &#40;4&#47;24&#41; Spn-positive episodes&#58; one episode was resistant to penicillin G&#44; cefpodoxime and erythromycin&#59; and three episodes were resistant to penicillin G&#44; amoxicillin&#44; cefpodoxime and erythromycin&#46; Multi-drug resistance was observed in six NTHi-positive episodes &#40;19&#37;&#41;&#46;</p></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Microbiological results in relation to vaccination status</span><p id="par0100" class="elsevierStylePara elsevierViewall">Of the 81 children positive for at least one pathogenic bacteria by combined culture<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>PCR&#44; 55 &#40;68&#37;&#41; children had received the appropriate PCV doses according to their age&#58; 61&#37; &#40;11&#47;18&#41; aged 3&#8211;10 months&#44; 64&#37; &#40;21&#47;33&#41; aged 11&#8211;22 months and 77&#37; &#40;23&#47;30&#41; aged 23&#8211;35 months&#46; The vaccines administered corresponded to PCV7 in 62 children &#40;77&#37;&#44; where 44 children &#91;71&#37;&#93; received &#8805;3 doses&#41;&#44; PCV10 in nine &#40;11&#37;&#44; where six children &#91;67&#37;&#93; received &#8805;3 doses&#41; and PCV13 in 17 children &#40;21&#37;&#44; where two children &#91;12&#37;&#93; received &#8805;3 doses&#41;&#46; There was no statistically significant difference in terms of vaccination between Hi-positive &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>1&#46;000&#41; and Spn-positive children &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;9497&#41;&#46;</p></span></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Discussion</span><p id="par0105" class="elsevierStylePara elsevierViewall">This study identified and characterized the bacterial pathogens associated with AOM in Spanish children less than 3 years of age during the post-PCV era&#46; We found a relatively high percentage &#40;69&#37;&#41; of episodes culture-positive for at least one pathogenic bacterium by combined culture and PCR&#46; Hi and Spn were the most common pathogens isolated from AOM episodes&#46; This finding is in agreement with previous studies conducted in Mexico&#44; Columbia&#44; Venezuela&#44; Germany and Spain that have implicated these pathogens as the main causative agents of AOM&#46;<a class="elsevierStyleCrossRefs" href="#bib0230"><span class="elsevierStyleSup">17&#8211;21</span></a> Interestingly&#44; similar proportions of Spn and Hi detected by culture and PCR highlight the relevance of PCR as an effective detection method&#46; This is corroborated by a former study that identified Spn and Hi in culture-negative samples using PCR-based diagnosis&#46;<a class="elsevierStyleCrossRef" href="#bib0255"><span class="elsevierStyleSup">22</span></a></p><p id="par0110" class="elsevierStylePara elsevierViewall">Spn and NTHi are highly responsible for the vast number of AOM cases throughout the world&#44; which has not changed significantly in the past fifty years before or after PCV introduction&#46;<a class="elsevierStyleCrossRefs" href="#bib0230"><span class="elsevierStyleSup">17&#44;23</span></a> Widespread vaccination with PCV could result in a lower role in AOM for Spn&#46; This could in turn result in NTHi becoming a more important pathogen in the years to come&#46;<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">23</span></a></p><p id="par0115" class="elsevierStylePara elsevierViewall">At least one dose of a Hi-type B vaccine was received by 112 children &#40;95&#46;7&#37;&#41;&#46; In our study&#44; NTHi was responsible for all Hi-positive episodes&#44; as has previously been observed in etiology studies where the majority or all Hi-positive episodes were non-typeable&#46;<a class="elsevierStyleCrossRefs" href="#bib0230"><span class="elsevierStyleSup">17&#44;18&#44;20&#44;24</span></a> Vaccination with Hi-type B could result in a lower incidence of Hi-type B as pathogen causing AOM&#44; but has not been able to reduce the incidence of NTHi&#46; The most common pneumococcal serotype was 19F followed by 19A in this study&#46; An earlier study from Spain also found 19F as the predominant serotype followed by 23F which was not found in this study&#46;<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">21</span></a> The persistence of 19F is noteworthy and underscores the lack of vaccine efficacy against this serotype in Spain&#46; Although serotype distribution varies geographically&#44; studies in Spain and several other countries have emphasized the predominance of serotype 19A after PCV introduction&#46;<a class="elsevierStyleCrossRefs" href="#bib0250"><span class="elsevierStyleSup">21&#44;25&#8211;27</span></a> The presence of 19A in this study possibly indicates insufficient cross-protection against this serotype by PCVs&#46; Although Spn and NTHi are the main causative pathogens of AOM&#44; and with Spn being the less predominant pathogen&#44; some papers have recently highlighted a shifting of pathogens&#44; with Spn being less frequent than NTHi in young children&#44; in concordance with our data&#46;<a class="elsevierStyleCrossRefs" href="#bib0260"><span class="elsevierStyleSup">23&#44;28</span></a></p><p id="par0120" class="elsevierStylePara elsevierViewall">As more than 80&#37; of physicians prescribed an antibiotic as initial treatment for patients with AOM in Spain&#44;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">5</span></a> high antibiotic resistance is expected&#46;<a class="elsevierStyleCrossRef" href="#bib0290"><span class="elsevierStyleSup">29</span></a> However&#44; in this study we observed that the majority of Spn and Hi-positive episodes were susceptible to amoxicillin and amoxicillin&#47;clavulanic acid&#44; respectively&#46; This finding supports the continued use of amoxicillin or amoxicillin&#47;clavulanic as the first line of AOM treatment in Spain&#46;<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">7</span></a> Also&#44; all Spn<span class="elsevierStyleItalic">-</span> and Hi-positive episodes were susceptible to cefotaxime&#46; Our antibacterial susceptibility patterns are similar to previous findings in Spain<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">21</span></a> and may be helpful when considering antibiotic management in Spain&#46;</p><p id="par0125" class="elsevierStylePara elsevierViewall">The AOM cases in this study may not be representative of all AOM cases in the Spanish population and the results should be considered as merely descriptive&#46; Although tympanocentesis is an effective method for identifying bacterial pathogens from the middle ear&#44; it is not routinely performed in Spain and contributed to our relatively small enrollment numbers and inability to draw strong conclusions&#46; Furthermore&#44; our study excluded recurrent AOM cases and included only the first AOM episode&#44; which could be another limiting factor given that in Spain&#44; it is difficult to perform tympanocentesis on the first AOM episode&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">Our study shows that in this post-PCV era&#44; NTHi and Spn were the leading bacterial etiological agents of AOM in young Spanish children&#44; and shifting pathogens has been observed where NTHi has emerged as an important pathogen of AOM&#46; Further studies are needed to evaluate the impact of pneumococcal vaccination on AOM disease burden&#44; after higher vaccination coverage rate is reached&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">Trademarks</span><p id="par0135" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Prevenar 7 and Prevenar 13</span> are registered trademarks of Pfizer&#47;Wyeth&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Synflorix</span>&#8482; is a registered trademark of the GSK group of companies&#46;</p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0125">Funding</span><p id="par0145" class="elsevierStylePara elsevierViewall">This study &#40;GSK study identifier&#58; 111425&#41; was sponsored by GlaxoSmithKline Biologicals SA&#46; GlaxoSmithKline Biologicals SA also funded all costs associated with the development and publishing of the manuscript&#46;</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0130">Authorship</span><p id="par0150" class="elsevierStylePara elsevierViewall">All authors had full access to the data and were involved in revising the manuscript critically for important intellectual content&#44; and gave final approval&#46; The corresponding author had final responsibility to submit for publication&#46; A confidentiality agreement was set between the authors and the sponsor&#46;</p></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0135">Conflict of interest</span><p id="par0155" class="elsevierStylePara elsevierViewall">Sistiaga-Hernando A&#44; Garc&#237;a-Corbeira P&#44; McCoig C and Devadiga R are employees of the GSK group of companies&#46; Sistiaga-Hernando A&#44; Garc&#237;a-Corbeira P and McCoig C report ownership of stock options&#47;restricted shares from the GSK group of companies&#46; Pumarola F reports payments received from the GSK group of companies for congress registration fees&#46; G&#243;mez Mart&#237;nez JR and Iniesta Turpin J report investigator fees received by their institution from the GSK group of companies during the conduct of the study&#46; Salamanca de la Cueva I&#44; Moraga-Llop FA&#44; Cardel&#250;s S and Rosell Ferrer R report no conflict of interest&#46;</p></span></span>"
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          "titulo" => "Introduction"
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              "titulo" => "Bacterial identification and antibacterial susceptibility testing"
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              "titulo" => "Statistical analyses"
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          "titulo" => "Results"
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              "titulo" => "Study participants and demographics"
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    "fechaRecibido" => "2015-06-28"
    "fechaAceptado" => "2015-10-28"
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          "clase" => "keyword"
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          "palabras" => array:4 [
            0 => "Acute otitis media"
            1 => "Children"
            2 => "Spain"
            3 => "Pneumococcal conjugate vaccine"
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          "palabras" => array:4 [
            0 => "Otitis media aguda"
            1 => "Ni&#241;os"
            2 => "Espa&#241;a"
            3 => "Vacuna neumoc&#243;cica conjugada"
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    "resumen" => array:2 [
      "en" => array:3 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Acute otitis media &#40;AOM&#41; is common in children aged &#60;3 years&#46; A pneumococcal conjugate vaccine &#40;PCV&#41; &#40;PCV7&#59; <span class="elsevierStyleItalic">Prevenar</span>&#44; Pfizer&#47;Wyeth&#44; USA&#41; has been available in Spain since 2001&#44; which has a coverage rate of 50&#8211;60&#37; in children aged &#60;5 years&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Materials and methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Children aged &#8805;3 to 36 months with AOM confirmed by an ear-nose-throat specialist were enrolled at seven centers in Spain &#40;February 2009&#8211;May 2012&#41; &#40;GSK study identifier&#58; 111425&#41;&#46; Middle-ear-fluid samples were collected by tympanocentesis or spontaneous otorrhea and cultured for bacterial identification&#46; Culture-negative samples were further analyzed using polymerase chain reaction &#40;PCR&#41;&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Of 125 confirmed AOM episodes in 124 children&#44; 117 were analyzed &#40;median age&#58; 17 months &#40;range&#58; 3&#8211;35&#41;&#59; eight AOM episodes were excluded from analyses&#46; Overall&#44; 69&#37; &#40;81&#47;117&#41; episodes were combined culture- and PCR-positive for &#8805;1 bacterial pathogen&#59; 44&#37; &#40;52&#47;117&#41; and 39&#37; &#40;46&#47;117&#41; were positive for <span class="elsevierStyleItalic">Haemophilus influenzae</span> &#40;Hi&#41; and <span class="elsevierStyleItalic">Streptococcus pneumoniae</span> &#40;Spn&#41;&#44; respectively&#46; 77 of 117 episodes were cultured for &#8805;1 bacteria&#44; of which 63 were culture-positive&#59; most commonly Spn &#40;24&#47;77&#59; 31&#37;&#41; and Hi &#40;32&#47;77&#59; 42&#37;&#41;&#46; PCR on culture-negative episodes identified 48&#37; Hi- and 55&#37; Spn-positive episodes&#46; The most common Spn serotype was 19F &#40;4&#47;24&#59; 17&#37;&#41; followed by 19A &#40;3&#47;24&#59; 13&#37;&#41;&#59; all Hi-positive episodes were non-typeable &#40;NTHi&#41;&#46; 81&#47;117 AOM episodes &#40;69&#37;&#41; occurred in children who had received &#8805;1 pneumococcal vaccine dose&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">NTHi and Spn were the main etiological agents for AOM in Spain&#46; Impact of pneumococcal vaccination on AOM requires further evaluation in Spain&#44; after higher vaccination coverage rate is reached&#46;</p></span>"
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          0 => array:2 [
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            "titulo" => "Materials and methods"
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        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducci&#243;n</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">La otitis media aguda &#40;OMA&#41; es com&#250;n en ni&#241;os menores de 3 a&#241;os&#46; En Espa&#241;a hay disponible una vacuna neumoc&#243;cica conjugada &#40;VNC&#41; &#40;VNC7&#59; <span class="elsevierStyleItalic">Prevenar</span>&#44; Pfizer&#47;Wyeth&#44; EE&#46; UU&#46;&#41; desde 2001&#44; habi&#233;ndose alcanzado una cobertura vacunal del 50-60&#37; en ni&#241;os menores de 5 a&#241;os&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Materiales y m&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Se reclut&#243; a ni&#241;os de 3 a 36 meses con OMA confirmada por especialista en otorrinolaringolog&#237;a en 7 centros espa&#241;oles &#40;febrero 2009-mayo 2012&#41; &#40;Proyectoe GSK&#58; 111425&#41;&#46; Se obtuvieron muestras de exudado del o&#237;do medio mediante timpanocentesis o de otorrea espont&#225;nea&#44; y se hizo cultivo para identificaci&#243;n bacteriana&#46; En muestras con cultivos negativos se realiz&#243; an&#225;lisis adicional mediante reacci&#243;n en cadena de la polimerasa &#40;PCR&#41;&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">De 125 episodios de OMA confirmados en 124 ni&#241;os&#44; se analizaron 117 &#40;edad mediana&#58; 17 meses &#91;rango&#58; 3&#8211;35&#93;&#41;&#59; 8 episodios de OMA fueron excluidos del an&#225;lisis&#46; En total&#44; combinando resultados de cultivo y PCR&#44; se identificaron uno o m&#225;s pat&#243;genos bacterianos en el 69&#37; &#40;81&#47;117&#41; de los episodios&#59; identific&#225;ndose <span class="elsevierStyleItalic">Haemophilus influenzae</span> &#40;Hi&#41; en el 44&#37; &#40;52&#47;117&#41; y <span class="elsevierStyleItalic">Streptococcus pneumoniae</span> &#40;Spn&#41; en el 39&#37; &#40;46&#47;117&#41;&#46; En 77 de los 117 episodios se hizo cultivo para uno o m&#225;s pat&#243;genos&#44; resultando positivo en 63&#44; con mayor frecuencia para Spn &#40;24&#47;77&#59; 31&#37;&#41; e Hi &#40;32&#47;77&#59; 42&#37;&#41;&#46; La PCR en episodios con cultivos negativos detect&#243; Hi en el 48&#37; y Spn en el 55&#37; de las muestras&#46; El serotipo de Spn m&#225;s com&#250;n fue el 19F &#40;4&#47;24&#59; 17&#37;&#41; seguido del 19A &#40;3&#47;24&#59; 13&#37;&#41;&#59; todos los episodios en los que se identific&#243; Hi correspondieron a Hi no tipificable &#40;HiNT&#41;&#46; Un total de 81&#47;117 episodios de OMA &#40;69&#37;&#41; se presentaron en ni&#241;os que hab&#237;an recibido una o m&#225;s dosis de vacuna antineumoc&#243;cica&#46;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">HiNT y Spn resultaron ser los principales agentes etiol&#243;gicos de la OMA en Espa&#241;a&#46; Para conocer el impacto de la vacunaci&#243;n antineumoc&#243;cica en la OMA en Espa&#241;a har&#225;n falta estudios adicionales cuando se haya alcanzado un nivel de cobertura mayor&#46;</p></span>"
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        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0015">Please cite this article as&#58; Pumarola F&#44; Salamanca de la Cueva I&#44; Sistiaga-Hernando A&#44; Garc&#237;a-Corbeira P&#44; Moraga-Llop FA&#44; Cardel&#250;s S&#44; et al&#46; Etiolog&#237;a bacteriana de la otitis media aguda en espa&#241;a en la era de la vacuna neumoc&#243;cica conjugada&#46; An Pediatr &#40;Barc&#41;&#46; 2016&#59;85&#58;224&#8211;231&#46;</p>"
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        "nota" => "<p class="elsevierStyleNotepara" id="npar0020">Upon presentation orally at the National Congress of the Spanish Society of Otorhinolaryngology and Cervical-Facial Pathology &#40;SEORL-PCF&#41; held in Madrid &#40;Spain&#41; from 25 to 28 October 2013&#46;</p>"
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Serogroup and serotype distribution of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>24&#41;&#46; <span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes with respective serotypes and serogroups&#46; OTHER<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>serogroups 8&#44; 15&#44; 22&#44; 35 &#40;1 episode each&#41; and 21 &#40;2 episodes&#41;&#46;</p>"
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          "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Antibacterial susceptibility of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>24&#41;&#46;</p>"
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          "leyenda" => "<p id="spar0065" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>total number of subjects&#46;</p><p id="spar0070" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number &#40;percentage&#41; of subjects in each group&#46;</p>"
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                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Characteristics&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Categories&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Age groups &#40;N</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">&#61;</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">117&#41;</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="3" align="left" valign="top">Age &#40;months&#41;</td><td class="td" title="table-entry  " align="left" valign="top">03&#8211;10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">25 &#40;21&#46;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">11&#8211;22&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">54 &#40;46&#46;2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">23&#8211;35&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">38 &#40;32&#46;5&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Summary of vaccination history &#40;N</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">&#61;</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">117&#41;</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Child received at least one dose of a&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">81 &#40;69&#46;2&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="3" align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Child received at least one dose of a <span class="elsevierStyleItalic">Haemophilus influenzae</span>-type B vaccine</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">112 &#40;95&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">No&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="3" align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Child received at least one dose of a Influenza vaccine</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">116 &#40;99&#46;1&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;0&#46;9&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Antibiotic treatment &#40;N</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">&#61;</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">117&#41;</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="2" align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Antibiotics taken before the sample was obtained</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">117 &#40;100&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="2" align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Antibiotics taken within the past one month</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7 &#40;6&#46;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">110 &#40;94&#46;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab1233409.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Characteristics of enrolled children&#46;</p>"
        ]
      ]
      4 => array:7 [
        "identificador" => "tbl0010"
        "etiqueta" => "Table 2"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0080" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number of episodes analyzed according to culture&#44; PCR or both&#46;</p><p id="spar0085" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">n</span>&#8242; &#40;&#37;&#41;<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>numbers &#40;percentage&#41; of episodes positive for each bacterium&#46;</p><p id="spar0090" class="elsevierStyleSimplePara elsevierViewall">There were two AOM episodes that were tested by both culture and PCR&#46;</p><p id="spar0095" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">Note</span>&#58; Culture-negative episodes were not tested for <span class="elsevierStyleItalic">Streptococcus pyogenes</span> and <span class="elsevierStyleItalic">Moraxella catarrhalis</span> by PCR&#46;</p><p id="spar0100" class="elsevierStyleSimplePara elsevierViewall">AOM&#44; acute otitis media&#59; PCR&#44; polymerase chain reaction&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Culture &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>77&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">PCR &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>42&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Combined culture<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>PCR &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>117&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span>&#8242; &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span>&#8242; &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span>&#8242; &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Positive for at least 1 pathogenic bacteria&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">63 &#40;82&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">29 &#40;69&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">81 &#40;69&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleItalic">Streptococcus pneumoniae</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">24 &#40;31&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">23 &#40;55&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">46 &#40;39&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleItalic">Haemophilus influenzae</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">32 &#40;42&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">20 &#40;48&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">52 &#40;44&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleItalic">Streptococcus pyogenes</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleItalic">Moraxella catarrhalis</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;1&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Co-infection with <span class="elsevierStyleItalic">Streptococcus pneumoniae</span> and <span class="elsevierStyleItalic">Haemophilus influenzae</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3 &#40;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">14 &#40;33&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">17 &#40;15&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab1233406.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0075" class="elsevierStyleSimplePara elsevierViewall">Bacterial identification of AOM episodes by culture and PCR&#46;</p>"
        ]
      ]
      5 => array:7 [
        "identificador" => "tbl0015"
        "etiqueta" => "Table 3"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:3 [
          "leyenda" => "<p id="spar0110" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes vaccinated and unvaccinated with pneumococcal conjugate vaccine&#46;</p><p id="spar0115" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number &#40;percentage&#41; of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes in each category&#46;</p>"
          "tablatextoimagen" => array:2 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td-with-role" title="table-head ; entry_with_role_rowhead " align="left" valign="top" scope="col">PCV name&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Serotypes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Vaccinated total &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>16&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " rowspan="6" align="left" valign="top">PCV7</td><td class="td" title="table-entry  " align="left" valign="top">14<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">19A&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3 &#40;19&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">19C&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">19F<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Other &#40;8&#44; 15&#44; 21&#44; 35&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4 &#40;25&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">PCV13&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">Other &#40;21&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">PCV7 and PCV13&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">6B<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">PCV10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">Other A &#40;10A&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab1233408.png"
              ]
            ]
            1 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td-with-role" title="table-head ; entry_with_role_rowhead " align="left" valign="top" scope="col">Serotypes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Unvaccinated total &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>8&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">18F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">19F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;25&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">23B&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;25&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Other &#40;22&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Other B &#40;15B&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab1233407.png"
              ]
            ]
          ]
          "notaPie" => array:1 [
            0 => array:3 [
              "identificador" => "tblfn0005"
              "etiqueta" => "a"
              "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Serotypes 6B&#44; 14 and 19F are all present in PCV7&#44; PCV10 and PCV13&#46;</p> <p class="elsevierStyleNotepara" id="npar0010">PCV&#44; pneumococcal conjugate vaccine&#59; PCV7&#44; 7-valent PCV&#59; PCV10&#44; 10-valent PCV&#59; PCV13&#44; 13-valent PCV&#46;</p>"
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0105" class="elsevierStyleSimplePara elsevierViewall">Serogroups and serotypes of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes &#40;by culture&#41; by name of pneumococcal conjugate vaccination status &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>16&#41;&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:29 [
            0 => array:3 [
              "identificador" => "bib0150"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Prospects for a vaccine against otitis media"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "A&#46;W&#46; Cripps"
                            1 => "D&#46;C&#46; Otczyk"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1586/14760584.5.4.517"
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Original Article
Bacterial etiology of acute otitis media in Spain in the post-pneumococcal conjugate vaccine era
Etiología bacteriana de la otitis media aguda en españa en la era de la vacuna neumocócica conjugada
Felix Pumarolaa,
Corresponding author
fpumarol@vhebron.net

Corresponding author.
, Ignacio Salamanca de la Cuevab, Alessandra Sistiaga-Hernandoc, Pilar García-Corbeirac, Fernando A. Moraga-Llopd, Sara Cardelúse, Cynthia McCoigc, Justo Ramón Gómez Martínezf, Rosa Rosell Ferrerg, Jesús Iniesta Turpinh, Raghavendra Devadigai
a Hospital Vall d’Hebron, Unidad de ORL Pediátrica, Barcelona, Spain
b Instituto Hispalense de Pediatría, Unidad de Estudio e Investigación, Sevilla, Spain
c GSK Vaccines, Medical Department, Madrid, Spain
d Hospital Vall d’Hebron, Unidad de Enfermedades Infecciosas Pediátricas, Barcelona, Spain
e Hospital de San Juan de Dios, Esplugues de Llobregat, Barcelona, Spain
f Hospital Universitario Central de Asturias, Servicio de Otorrinolaringología, Oviedo, Spain
g Hospital Universitari Parc Taulí de Sabadell, Servicio de Otorrinolaringología, Sabadell, Barcelona, Spain
h Hospital Clínico Universitario Virgen de la Arrixaca, Servicio de Otorrinolaringología, El Palmar, Murcia, Spain
i GSK Pharmaceuticals Pvt. Ltd., Bangalore, India
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the annual incidence of AOM in children below 2 years of age is estimated at 392 per 1000 person-years&#44; as compared with 263 per 1000 person-years in children aged between 3 and 5 years&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">4</span></a> Antibiotics are prescribed as first-line treatment in more than 90&#37; of Spanish children presenting with symptoms of AOM &#40;e&#46;g&#46; ear discharge&#41;&#44; although an initial observation is recommended beforehand&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">5</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">Following the introduction of the 7-valent pneumococcal conjugate vaccine &#40;PCV&#41; &#40;PCV7&#59; <span class="elsevierStyleItalic">Prevenar 7</span>&#44; Pfizer&#47;Wyeth&#44; USA&#41; onto the Spanish private market in 2001&#44;<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">6</span></a> the vaccination coverage rate in children younger than 5 years had reached 50&#8211;60&#37; 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the underlying microbiology of infectious diseases changes over time as a consequence of vaccination and antibiotic consumption<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">9</span></a> and several global studies have documented a decrease in the circulation of pneumococcal vaccine serotypes and an increase in non-vaccine serotypes and Hi serotypes since PCV7 implementation&#46;<a class="elsevierStyleCrossRef" href="#bib0195"><span class="elsevierStyleSup">10</span></a> This study was designed to assess the bacterial etiology of AOM in Spanish children younger than 3 years of age using both culture and polymerase chain reaction &#40;PCR&#41;&#59; it complements a previous study describing the bacterial etiology of recurrent AOM and AOM treatment failures in Spanish children during the post-PCV era&#46;<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">7</span></a></p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Material and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Study design and participants</span><p id="par0025" class="elsevierStylePara elsevierViewall">This prospective&#44; epidemiological study was conducted at seven centres across Spain between February 2009 and May 2012 &#40;GSK study identifier&#58; 111425&#41;&#46; Children aged &#8805;3 to 36 months with ear&#8211;nose&#8211;throat specialist-confirmed AOM&#44; and who could provide a middle ear fluid sample &#40;MEF&#41;&#44; were enrolled in the study&#46; MEF samples were collected by tympanocentesis&#59; in case of otorrhea&#44; the samples were collected via deep aspiration of MEF through needle insertion after ear canal cleaning&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">AOM was confirmed based on the acute and sudden onset of the disease within the previous 3 days&#58; fever&#44; irritability&#44; intense erythema of the tympanum or earache&#59; and presence of effusion in the middle ear&#46; The presence of middle ear effusion was characterized by tympanum bulging&#44; limited or no mobility in the tympanum &#40;evaluated using pneumatic otoscopy&#41;&#44; fluid behind the tympanum and&#47;or otorrhea &#40;defined as perforation occurring within 48<span class="elsevierStyleHsp" style=""></span>h before enrollment&#41;&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">Children were excluded from participating in the study if they were hospitalized during the AOM episode &#40;to exclude possible confusion with intrahospital infections&#41; or in the event of otitis externa&#47;otitis media with effusion&#58; AOM for &#62;72<span class="elsevierStyleHsp" style=""></span>h or otorrhea for &#62;48<span class="elsevierStyleHsp" style=""></span>h before enrollment&#59; or in situ transtympanic aerators&#46; Children who received antibiotics for illnesses other than AOM during the 72-hour period before enrollment and were prescribed antibiotics before performing tympanocentesis were also excluded&#46; Finally&#44; patients with protocol-forbidden medical conditions were excluded&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">The study adhered to the principles of Good Clinical Practice&#44; including the 1964 Declaration of Helsinki and local Spanish rules and regulations&#46; The study was approved by the Institutional Review Board of each participating center and parents&#47;guardians provided written informed consent before enrollment&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Bacterial identification and antibacterial susceptibility testing</span><p id="par0045" class="elsevierStylePara elsevierViewall">MEF samples were collected by tympanocentesis performed under otoscopy through a reusable <span class="elsevierStyleItalic">CDT Aspirator</span> attached to a <span class="elsevierStyleItalic">CDT speculum</span>&#44; or by sampling of spontaneous otorrhea&#46; In cases of bilateral AOM&#44; MEF samples were collected from both ears&#46; Tympanocentesis was performed without anesthesia&#46; Aspirated MEF samples were inoculated onto Amies transport medium<a class="elsevierStyleCrossRef" href="#bib0200"><span class="elsevierStyleSup">11</span></a> and kept at room temperature for 16<span class="elsevierStyleHsp" style=""></span>h &#40;and up to a maximum of 48<span class="elsevierStyleHsp" style=""></span>h&#41; before subsequent analysis at a GSK-designated laboratory&#46; MEF samples were then inoculated onto chocolate &#40;with bacitracin for otorrhea samples&#41; and blood agar &#40;with nalidixic acid&#41;&#46; Bacterial identification was undertaken using standard bacteriological procedures&#46;<a class="elsevierStyleCrossRef" href="#bib0205"><span class="elsevierStyleSup">12</span></a> Serotyping for Spn was performed by Quellung&#39;s reaction and for Hi using monovalent anti-sera&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">Culture-negative samples were further analyzed using real-time PCR assay for the presence of the pneumococcal pneumolysin &#40;ply&#41; gene&#44;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">13</span></a> pneumococcal wzg gene&#44;<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">14</span></a> and the omp2 gene for non-capsulated and capsulated Hi&#46;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">15</span></a> Inhibited PCR reactions were all checked using a commercial exogenous internal positive control &#40;Applied Biosystems&#44; Foster City&#44; CA&#44; USA&#41;&#46; A subsequent extraction for eliminating inhibitors was performed on inhibited samples using 20&#37; &#40;w&#47;v&#41; Chelex-100 resin &#40;BioRad Laboratories&#44; Hercules&#44; CA&#44; USA&#41;&#46; The samples were further tested for antibacterial susceptibility&#46; Minimal inhibitory concentrations were derived using <span class="elsevierStyleItalic">Etest</span> &#40;bioM&#233;rieux&#44; France&#41; and interpreted with the criteria published by the Clinical and Laboratory Standards Institute in 2009&#46;<a class="elsevierStyleCrossRef" href="#bib0225"><span class="elsevierStyleSup">16</span></a></p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Statistical analyses</span><p id="par0055" class="elsevierStylePara elsevierViewall">Analyses were performed on children meeting all the selection criteria&#44; complying with protocol-defined procedures&#44; and from whom laboratory results of the MEF sample were available&#46; The proportion of AOM episodes caused by Spn&#44; Hi and other bacterial pathogens and the percentages of Spn and Hi serotypes were calculated&#46; Only the first AOM episode was included and any recurrent AOM cases were excluded from analyses&#46; The antibacterial susceptibility of Spn and Hi was determined&#46; History of previous PCV&#44; Hi-type B and influenza vaccinations was recorded&#46; All statistical analyses were performed using <span class="elsevierStyleItalic">SAS version 9&#46;2</span> &#40;SAS Institute Inc&#46;&#44; Cary&#44; NC&#44; USA&#41;&#46;</p></span></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Results</span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Study participants and demographics</span><p id="par0060" class="elsevierStylePara elsevierViewall">Of 125 AOM episodes in 124 subjects&#44; recorded during the study period&#44; 117 episodes &#40;tympanocentesis<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>91&#59; otorrhea<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>26&#41; &#40;from 117 subjects&#41; were analyzed&#46; Only one subject had two AOM episodes&#44; of which one episode was eliminated from the analyses&#46; Therefore&#44; a total of eight AOM episodes were excluded from the analyses&#58; protocol violation &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>1&#41;&#44; having a protocol-forbidden medical condition &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>4&#41; and missing MEF&#47;otorrhea sample &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#41;&#46; The median age of children was 17 months &#40;range&#58; 3&#8211;35&#41; and 60&#46;7&#37; were male&#46; AOM episodes were most frequently observed in children aged 11&#8211;22 months &#40;46&#46;2&#37;&#59; 54&#47;117&#41; followed by 23&#8211;35 months &#40;32&#46;5&#37;&#59; 38&#47;117&#41; and 3&#8211;10 months &#40;21&#46;4&#37;&#59; 25&#47;117&#41;&#46; At least one dose of a pneumococcal vaccine and Hi-type B vaccine was received by 69&#46;2&#37; and 95&#46;7&#37; subjects&#44; respectively&#46; None of the subjects received influenza vaccine&#46; <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a> presents information on age groups in the study&#44; summary of vaccination history and antibiotic treatment&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Bacterial identification</span><p id="par0065" class="elsevierStylePara elsevierViewall">The microbiological results are reported in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46; Bacterial culture tests were performed for 117 episodes&#46; Growth for any bacterium was cultured from 66&#37; &#40;77&#47;117&#41; of episodes&#58; 82&#37; of which &#40;63&#47;77&#41; were culture-positive for at least one bacterium under study &#40;Spn&#44; Hi&#44; <span class="elsevierStyleItalic">Streptococcus pyogenes</span> &#91;Spy&#93; or <span class="elsevierStyleItalic">Moraxella catarrhalis</span> &#91;Mcat&#93;&#41;&#46; The most frequent bacteria were Hi &#40;42&#37;&#59; 32&#47;77&#41; and Spn &#40;31&#37;&#59; 24&#47;77&#41;&#46; Spy was isolated in 10 samples and Mcat in 1 sample&#59; co-infection was detected in 10 episodes&#44; of which 4 co-infections involved bacterium under study&#58; Spn and Hi &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#41;&#59; Spy and Hi &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>1&#41;&#46; The remaining 40 episodes were culture-negative&#46; However&#44; PCR was performed for 42 episodes &#40;40 culture-negative episodes<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>2 culture-positive episodes&#41;&#44; of which 48&#37; &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>20&#41; were found to be Hi-positive and 55&#37; &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>23&#41; were Spn-positive&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0070" class="elsevierStylePara elsevierViewall">Combined culture and PCR results indicated that 52 AOM episodes &#40;44&#37;&#41; were Hi-positive and 46 &#40;39&#37;&#41; were Spn-positive&#59; 17 episodes &#40;15&#37;&#41; were positive for both Hi and Spn&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">The most common Spn serotypes amongst culture-positive episodes were 19F &#40;17&#37;&#59; 4&#47;24&#41; and 19A &#40;13&#37;&#59; 3&#47;24&#41; &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41;&#46; In episodes from children who had been previously vaccinated&#44; PCV7 vaccine serotypes &#40;14 and 19F&#41; were present in 19&#37; &#40;3&#47;16&#41; of episodes and PCV7&#47;PCV13 vaccine serotype &#40;6B&#41; was present in 6&#37; &#40;1&#47;16&#41; of episodes &#40;<a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>&#41;&#46; PCV7 vaccine serotype &#40;19F&#41; was present in 25&#37; &#40;2&#47;8&#41; episodes from unvaccinated children&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><elsevierMultimedia ident="tbl0015"></elsevierMultimedia><p id="par0080" class="elsevierStylePara elsevierViewall">All 32 Hi-positive episodes were non-typeable &#40;NTHi&#41;&#58; 69&#37; &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>22&#41; in vaccinated &#40;PCV vaccine&#41; and 31&#37; &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>10&#41; in unvaccinated &#40;PCV vaccine&#41; children&#46;</p></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Antibacterial susceptibility</span><p id="par0085" class="elsevierStylePara elsevierViewall">Of the 24 Spn-positive episodes&#44; all &#40;100&#37;&#41; were susceptible to cefotaxime&#44; 22 &#40;92&#37;&#41; to amoxicillin&#44; 17 &#40;71&#37;&#41; to erythromycin and 17 &#40;74&#37;&#41; to cefpodoxime &#40;since there was one missing Spn-positive episode susceptible to only cefpodoxime&#44; 23 of 24 Spn-positive episodes were considered for the analyses&#41;&#46; Ten episodes were susceptible and intermediately susceptible to penicillin G and four &#40;17&#37;&#41; were resistant to penicillin &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46; Serotype 19A was most commonly resistant to penicillin G&#44; amoxicillin&#44; cefpodoxime&#44; and erythromycin&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><p id="par0090" class="elsevierStylePara elsevierViewall">Of the 32 Hi-positive episodes&#44; all &#40;100&#37;&#41; were susceptible to both cefpodoxime and cefotaxime and 26 &#40;81&#37;&#41; to amoxicillin&#47;clavulanic acid&#46; Six episodes &#40;19&#37;&#41; were resistant to amoxicillin&#47;clavulanic acid &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>&#41;&#46;</p><elsevierMultimedia ident="fig0015"></elsevierMultimedia><p id="par0095" class="elsevierStylePara elsevierViewall">Multi-drug resistance was observed in 17&#37; &#40;4&#47;24&#41; Spn-positive episodes&#58; one episode was resistant to penicillin G&#44; cefpodoxime and erythromycin&#59; and three episodes were resistant to penicillin G&#44; amoxicillin&#44; cefpodoxime and erythromycin&#46; Multi-drug resistance was observed in six NTHi-positive episodes &#40;19&#37;&#41;&#46;</p></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Microbiological results in relation to vaccination status</span><p id="par0100" class="elsevierStylePara elsevierViewall">Of the 81 children positive for at least one pathogenic bacteria by combined culture<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>PCR&#44; 55 &#40;68&#37;&#41; children had received the appropriate PCV doses according to their age&#58; 61&#37; &#40;11&#47;18&#41; aged 3&#8211;10 months&#44; 64&#37; &#40;21&#47;33&#41; aged 11&#8211;22 months and 77&#37; &#40;23&#47;30&#41; aged 23&#8211;35 months&#46; The vaccines administered corresponded to PCV7 in 62 children &#40;77&#37;&#44; where 44 children &#91;71&#37;&#93; received &#8805;3 doses&#41;&#44; PCV10 in nine &#40;11&#37;&#44; where six children &#91;67&#37;&#93; received &#8805;3 doses&#41; and PCV13 in 17 children &#40;21&#37;&#44; where two children &#91;12&#37;&#93; received &#8805;3 doses&#41;&#46; There was no statistically significant difference in terms of vaccination between Hi-positive &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>1&#46;000&#41; and Spn-positive children &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;9497&#41;&#46;</p></span></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Discussion</span><p id="par0105" class="elsevierStylePara elsevierViewall">This study identified and characterized the bacterial pathogens associated with AOM in Spanish children less than 3 years of age during the post-PCV era&#46; We found a relatively high percentage &#40;69&#37;&#41; of episodes culture-positive for at least one pathogenic bacterium by combined culture and PCR&#46; Hi and Spn were the most common pathogens isolated from AOM episodes&#46; This finding is in agreement with previous studies conducted in Mexico&#44; Columbia&#44; Venezuela&#44; Germany and Spain that have implicated these pathogens as the main causative agents of AOM&#46;<a class="elsevierStyleCrossRefs" href="#bib0230"><span class="elsevierStyleSup">17&#8211;21</span></a> Interestingly&#44; similar proportions of Spn and Hi detected by culture and PCR highlight the relevance of PCR as an effective detection method&#46; This is corroborated by a former study that identified Spn and Hi in culture-negative samples using PCR-based diagnosis&#46;<a class="elsevierStyleCrossRef" href="#bib0255"><span class="elsevierStyleSup">22</span></a></p><p id="par0110" class="elsevierStylePara elsevierViewall">Spn and NTHi are highly responsible for the vast number of AOM cases throughout the world&#44; which has not changed significantly in the past fifty years before or after PCV introduction&#46;<a class="elsevierStyleCrossRefs" href="#bib0230"><span class="elsevierStyleSup">17&#44;23</span></a> Widespread vaccination with PCV could result in a lower role in AOM for Spn&#46; This could in turn result in NTHi becoming a more important pathogen in the years to come&#46;<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">23</span></a></p><p id="par0115" class="elsevierStylePara elsevierViewall">At least one dose of a Hi-type B vaccine was received by 112 children &#40;95&#46;7&#37;&#41;&#46; In our study&#44; NTHi was responsible for all Hi-positive episodes&#44; as has previously been observed in etiology studies where the majority or all Hi-positive episodes were non-typeable&#46;<a class="elsevierStyleCrossRefs" href="#bib0230"><span class="elsevierStyleSup">17&#44;18&#44;20&#44;24</span></a> Vaccination with Hi-type B could result in a lower incidence of Hi-type B as pathogen causing AOM&#44; but has not been able to reduce the incidence of NTHi&#46; The most common pneumococcal serotype was 19F followed by 19A in this study&#46; An earlier study from Spain also found 19F as the predominant serotype followed by 23F which was not found in this study&#46;<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">21</span></a> The persistence of 19F is noteworthy and underscores the lack of vaccine efficacy against this serotype in Spain&#46; Although serotype distribution varies geographically&#44; studies in Spain and several other countries have emphasized the predominance of serotype 19A after PCV introduction&#46;<a class="elsevierStyleCrossRefs" href="#bib0250"><span class="elsevierStyleSup">21&#44;25&#8211;27</span></a> The presence of 19A in this study possibly indicates insufficient cross-protection against this serotype by PCVs&#46; Although Spn and NTHi are the main causative pathogens of AOM&#44; and with Spn being the less predominant pathogen&#44; some papers have recently highlighted a shifting of pathogens&#44; with Spn being less frequent than NTHi in young children&#44; in concordance with our data&#46;<a class="elsevierStyleCrossRefs" href="#bib0260"><span class="elsevierStyleSup">23&#44;28</span></a></p><p id="par0120" class="elsevierStylePara elsevierViewall">As more than 80&#37; of physicians prescribed an antibiotic as initial treatment for patients with AOM in Spain&#44;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">5</span></a> high antibiotic resistance is expected&#46;<a class="elsevierStyleCrossRef" href="#bib0290"><span class="elsevierStyleSup">29</span></a> However&#44; in this study we observed that the majority of Spn and Hi-positive episodes were susceptible to amoxicillin and amoxicillin&#47;clavulanic acid&#44; respectively&#46; This finding supports the continued use of amoxicillin or amoxicillin&#47;clavulanic as the first line of AOM treatment in Spain&#46;<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">7</span></a> Also&#44; all Spn<span class="elsevierStyleItalic">-</span> and Hi-positive episodes were susceptible to cefotaxime&#46; Our antibacterial susceptibility patterns are similar to previous findings in Spain<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">21</span></a> and may be helpful when considering antibiotic management in Spain&#46;</p><p id="par0125" class="elsevierStylePara elsevierViewall">The AOM cases in this study may not be representative of all AOM cases in the Spanish population and the results should be considered as merely descriptive&#46; Although tympanocentesis is an effective method for identifying bacterial pathogens from the middle ear&#44; it is not routinely performed in Spain and contributed to our relatively small enrollment numbers and inability to draw strong conclusions&#46; Furthermore&#44; our study excluded recurrent AOM cases and included only the first AOM episode&#44; which could be another limiting factor given that in Spain&#44; it is difficult to perform tympanocentesis on the first AOM episode&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">Our study shows that in this post-PCV era&#44; NTHi and Spn were the leading bacterial etiological agents of AOM in young Spanish children&#44; and shifting pathogens has been observed where NTHi has emerged as an important pathogen of AOM&#46; Further studies are needed to evaluate the impact of pneumococcal vaccination on AOM disease burden&#44; after higher vaccination coverage rate is reached&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">Trademarks</span><p id="par0135" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Prevenar 7 and Prevenar 13</span> are registered trademarks of Pfizer&#47;Wyeth&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Synflorix</span>&#8482; is a registered trademark of the GSK group of companies&#46;</p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0125">Funding</span><p id="par0145" class="elsevierStylePara elsevierViewall">This study &#40;GSK study identifier&#58; 111425&#41; was sponsored by GlaxoSmithKline Biologicals SA&#46; GlaxoSmithKline Biologicals SA also funded all costs associated with the development and publishing of the manuscript&#46;</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0130">Authorship</span><p id="par0150" class="elsevierStylePara elsevierViewall">All authors had full access to the data and were involved in revising the manuscript critically for important intellectual content&#44; and gave final approval&#46; The corresponding author had final responsibility to submit for publication&#46; A confidentiality agreement was set between the authors and the sponsor&#46;</p></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0135">Conflict of interest</span><p id="par0155" class="elsevierStylePara elsevierViewall">Sistiaga-Hernando A&#44; Garc&#237;a-Corbeira P&#44; McCoig C and Devadiga R are employees of the GSK group of companies&#46; Sistiaga-Hernando A&#44; Garc&#237;a-Corbeira P and McCoig C report ownership of stock options&#47;restricted shares from the GSK group of companies&#46; Pumarola F reports payments received from the GSK group of companies for congress registration fees&#46; G&#243;mez Mart&#237;nez JR and Iniesta Turpin J report investigator fees received by their institution from the GSK group of companies during the conduct of the study&#46; Salamanca de la Cueva I&#44; Moraga-Llop FA&#44; Cardel&#250;s S and Rosell Ferrer R report no conflict of interest&#46;</p></span></span>"
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    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2015-06-28"
    "fechaAceptado" => "2015-10-28"
    "PalabrasClave" => array:2 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec749893"
          "palabras" => array:4 [
            0 => "Acute otitis media"
            1 => "Children"
            2 => "Spain"
            3 => "Pneumococcal conjugate vaccine"
          ]
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        0 => array:4 [
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          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec749894"
          "palabras" => array:4 [
            0 => "Otitis media aguda"
            1 => "Ni&#241;os"
            2 => "Espa&#241;a"
            3 => "Vacuna neumoc&#243;cica conjugada"
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        ]
      ]
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    "tieneResumen" => true
    "resumen" => array:2 [
      "en" => array:3 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Acute otitis media &#40;AOM&#41; is common in children aged &#60;3 years&#46; A pneumococcal conjugate vaccine &#40;PCV&#41; &#40;PCV7&#59; <span class="elsevierStyleItalic">Prevenar</span>&#44; Pfizer&#47;Wyeth&#44; USA&#41; has been available in Spain since 2001&#44; which has a coverage rate of 50&#8211;60&#37; in children aged &#60;5 years&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Materials and methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Children aged &#8805;3 to 36 months with AOM confirmed by an ear-nose-throat specialist were enrolled at seven centers in Spain &#40;February 2009&#8211;May 2012&#41; &#40;GSK study identifier&#58; 111425&#41;&#46; Middle-ear-fluid samples were collected by tympanocentesis or spontaneous otorrhea and cultured for bacterial identification&#46; Culture-negative samples were further analyzed using polymerase chain reaction &#40;PCR&#41;&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Of 125 confirmed AOM episodes in 124 children&#44; 117 were analyzed &#40;median age&#58; 17 months &#40;range&#58; 3&#8211;35&#41;&#59; eight AOM episodes were excluded from analyses&#46; Overall&#44; 69&#37; &#40;81&#47;117&#41; episodes were combined culture- and PCR-positive for &#8805;1 bacterial pathogen&#59; 44&#37; &#40;52&#47;117&#41; and 39&#37; &#40;46&#47;117&#41; were positive for <span class="elsevierStyleItalic">Haemophilus influenzae</span> &#40;Hi&#41; and <span class="elsevierStyleItalic">Streptococcus pneumoniae</span> &#40;Spn&#41;&#44; respectively&#46; 77 of 117 episodes were cultured for &#8805;1 bacteria&#44; of which 63 were culture-positive&#59; most commonly Spn &#40;24&#47;77&#59; 31&#37;&#41; and Hi &#40;32&#47;77&#59; 42&#37;&#41;&#46; PCR on culture-negative episodes identified 48&#37; Hi- and 55&#37; Spn-positive episodes&#46; The most common Spn serotype was 19F &#40;4&#47;24&#59; 17&#37;&#41; followed by 19A &#40;3&#47;24&#59; 13&#37;&#41;&#59; all Hi-positive episodes were non-typeable &#40;NTHi&#41;&#46; 81&#47;117 AOM episodes &#40;69&#37;&#41; occurred in children who had received &#8805;1 pneumococcal vaccine dose&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">NTHi and Spn were the main etiological agents for AOM in Spain&#46; Impact of pneumococcal vaccination on AOM requires further evaluation in Spain&#44; after higher vaccination coverage rate is reached&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0005"
            "titulo" => "Introduction"
          ]
          1 => array:2 [
            "identificador" => "abst0010"
            "titulo" => "Materials and methods"
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          2 => array:2 [
            "identificador" => "abst0015"
            "titulo" => "Results"
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          3 => array:2 [
            "identificador" => "abst0020"
            "titulo" => "Conclusions"
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        "titulo" => "Resumen"
        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducci&#243;n</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">La otitis media aguda &#40;OMA&#41; es com&#250;n en ni&#241;os menores de 3 a&#241;os&#46; En Espa&#241;a hay disponible una vacuna neumoc&#243;cica conjugada &#40;VNC&#41; &#40;VNC7&#59; <span class="elsevierStyleItalic">Prevenar</span>&#44; Pfizer&#47;Wyeth&#44; EE&#46; UU&#46;&#41; desde 2001&#44; habi&#233;ndose alcanzado una cobertura vacunal del 50-60&#37; en ni&#241;os menores de 5 a&#241;os&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Materiales y m&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Se reclut&#243; a ni&#241;os de 3 a 36 meses con OMA confirmada por especialista en otorrinolaringolog&#237;a en 7 centros espa&#241;oles &#40;febrero 2009-mayo 2012&#41; &#40;Proyectoe GSK&#58; 111425&#41;&#46; Se obtuvieron muestras de exudado del o&#237;do medio mediante timpanocentesis o de otorrea espont&#225;nea&#44; y se hizo cultivo para identificaci&#243;n bacteriana&#46; En muestras con cultivos negativos se realiz&#243; an&#225;lisis adicional mediante reacci&#243;n en cadena de la polimerasa &#40;PCR&#41;&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">De 125 episodios de OMA confirmados en 124 ni&#241;os&#44; se analizaron 117 &#40;edad mediana&#58; 17 meses &#91;rango&#58; 3&#8211;35&#93;&#41;&#59; 8 episodios de OMA fueron excluidos del an&#225;lisis&#46; En total&#44; combinando resultados de cultivo y PCR&#44; se identificaron uno o m&#225;s pat&#243;genos bacterianos en el 69&#37; &#40;81&#47;117&#41; de los episodios&#59; identific&#225;ndose <span class="elsevierStyleItalic">Haemophilus influenzae</span> &#40;Hi&#41; en el 44&#37; &#40;52&#47;117&#41; y <span class="elsevierStyleItalic">Streptococcus pneumoniae</span> &#40;Spn&#41; en el 39&#37; &#40;46&#47;117&#41;&#46; En 77 de los 117 episodios se hizo cultivo para uno o m&#225;s pat&#243;genos&#44; resultando positivo en 63&#44; con mayor frecuencia para Spn &#40;24&#47;77&#59; 31&#37;&#41; e Hi &#40;32&#47;77&#59; 42&#37;&#41;&#46; La PCR en episodios con cultivos negativos detect&#243; Hi en el 48&#37; y Spn en el 55&#37; de las muestras&#46; El serotipo de Spn m&#225;s com&#250;n fue el 19F &#40;4&#47;24&#59; 17&#37;&#41; seguido del 19A &#40;3&#47;24&#59; 13&#37;&#41;&#59; todos los episodios en los que se identific&#243; Hi correspondieron a Hi no tipificable &#40;HiNT&#41;&#46; Un total de 81&#47;117 episodios de OMA &#40;69&#37;&#41; se presentaron en ni&#241;os que hab&#237;an recibido una o m&#225;s dosis de vacuna antineumoc&#243;cica&#46;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">HiNT y Spn resultaron ser los principales agentes etiol&#243;gicos de la OMA en Espa&#241;a&#46; Para conocer el impacto de la vacunaci&#243;n antineumoc&#243;cica en la OMA en Espa&#241;a har&#225;n falta estudios adicionales cuando se haya alcanzado un nivel de cobertura mayor&#46;</p></span>"
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      0 => array:2 [
        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0015">Please cite this article as&#58; Pumarola F&#44; Salamanca de la Cueva I&#44; Sistiaga-Hernando A&#44; Garc&#237;a-Corbeira P&#44; Moraga-Llop FA&#44; Cardel&#250;s S&#44; et al&#46; Etiolog&#237;a bacteriana de la otitis media aguda en espa&#241;a en la era de la vacuna neumoc&#243;cica conjugada&#46; An Pediatr &#40;Barc&#41;&#46; 2016&#59;85&#58;224&#8211;231&#46;</p>"
      ]
      1 => array:2 [
        "etiqueta" => "&#9734;&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0020">Upon presentation orally at the National Congress of the Spanish Society of Otorhinolaryngology and Cervical-Facial Pathology &#40;SEORL-PCF&#41; held in Madrid &#40;Spain&#41; from 25 to 28 October 2013&#46;</p>"
      ]
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Serogroup and serotype distribution of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>24&#41;&#46; <span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes with respective serotypes and serogroups&#46; OTHER<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>serogroups 8&#44; 15&#44; 22&#44; 35 &#40;1 episode each&#41; and 21 &#40;2 episodes&#41;&#46;</p>"
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          "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Antibacterial susceptibility of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>24&#41;&#46;</p>"
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          "leyenda" => "<p id="spar0065" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>total number of subjects&#46;</p><p id="spar0070" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number &#40;percentage&#41; of subjects in each group&#46;</p>"
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                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Characteristics&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Categories&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Age groups &#40;N</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">&#61;</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">117&#41;</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="3" align="left" valign="top">Age &#40;months&#41;</td><td class="td" title="table-entry  " align="left" valign="top">03&#8211;10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">25 &#40;21&#46;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">11&#8211;22&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">54 &#40;46&#46;2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">23&#8211;35&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">38 &#40;32&#46;5&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Summary of vaccination history &#40;N</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">&#61;</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">117&#41;</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Child received at least one dose of a&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">81 &#40;69&#46;2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pneumococcal vaccine&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">36 &#40;30&#46;8&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="3" align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Child received at least one dose of a <span class="elsevierStyleItalic">Haemophilus influenzae</span>-type B vaccine</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">112 &#40;95&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3 &#40;2&#46;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;1&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="3" align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Child received at least one dose of a Influenza vaccine</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">116 &#40;99&#46;1&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;0&#46;9&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Antibiotic treatment &#40;N</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">&#61;</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">117&#41;</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="2" align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Antibiotics taken before the sample was obtained</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">117 &#40;100&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " rowspan="2" align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Antibiotics taken within the past one month</td><td class="td" title="table-entry  " align="left" valign="top">Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7 &#40;6&#46;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">110 &#40;94&#46;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
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          "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Characteristics of enrolled children&#46;</p>"
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        "tabla" => array:2 [
          "leyenda" => "<p id="spar0080" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number of episodes analyzed according to culture&#44; PCR or both&#46;</p><p id="spar0085" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">n</span>&#8242; &#40;&#37;&#41;<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>numbers &#40;percentage&#41; of episodes positive for each bacterium&#46;</p><p id="spar0090" class="elsevierStyleSimplePara elsevierViewall">There were two AOM episodes that were tested by both culture and PCR&#46;</p><p id="spar0095" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">Note</span>&#58; Culture-negative episodes were not tested for <span class="elsevierStyleItalic">Streptococcus pyogenes</span> and <span class="elsevierStyleItalic">Moraxella catarrhalis</span> by PCR&#46;</p><p id="spar0100" class="elsevierStyleSimplePara elsevierViewall">AOM&#44; acute otitis media&#59; PCR&#44; polymerase chain reaction&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Culture &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>77&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">PCR &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>42&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Combined culture<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>PCR &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>117&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span>&#8242; &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span>&#8242; &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span>&#8242; &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Positive for at least 1 pathogenic bacteria&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">63 &#40;82&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">29 &#40;69&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">81 &#40;69&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleItalic">Streptococcus pneumoniae</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">24 &#40;31&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">23 &#40;55&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">46 &#40;39&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleItalic">Haemophilus influenzae</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">32 &#40;42&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">20 &#40;48&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">52 &#40;44&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleItalic">Streptococcus pyogenes</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleItalic">Moraxella catarrhalis</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;1&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Co-infection with <span class="elsevierStyleItalic">Streptococcus pneumoniae</span> and <span class="elsevierStyleItalic">Haemophilus influenzae</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3 &#40;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">14 &#40;33&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">17 &#40;15&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
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                0 => "xTab1233406.png"
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        "descripcion" => array:1 [
          "en" => "<p id="spar0075" class="elsevierStyleSimplePara elsevierViewall">Bacterial identification of AOM episodes by culture and PCR&#46;</p>"
        ]
      ]
      5 => array:7 [
        "identificador" => "tbl0015"
        "etiqueta" => "Table 3"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:3 [
          "leyenda" => "<p id="spar0110" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes vaccinated and unvaccinated with pneumococcal conjugate vaccine&#46;</p><p id="spar0115" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>number &#40;percentage&#41; of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes in each category&#46;</p>"
          "tablatextoimagen" => array:2 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td-with-role" title="table-head ; entry_with_role_rowhead " align="left" valign="top" scope="col">PCV name&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Serotypes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Vaccinated total &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>16&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " rowspan="6" align="left" valign="top">PCV7</td><td class="td" title="table-entry  " align="left" valign="top">14<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">19A&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3 &#40;19&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">19C&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">19F<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Other &#40;8&#44; 15&#44; 21&#44; 35&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4 &#40;25&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">PCV13&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">Other &#40;21&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">PCV7 and PCV13&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">6B<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">PCV10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">Other A &#40;10A&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
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                0 => "xTab1233408.png"
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            1 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td-with-role" title="table-head ; entry_with_role_rowhead " align="left" valign="top" scope="col">Serotypes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col">Unvaccinated total &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>8&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">18F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">19F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;25&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">23B&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;25&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Other &#40;22&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Other B &#40;15B&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
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                0 => "xTab1233407.png"
              ]
            ]
          ]
          "notaPie" => array:1 [
            0 => array:3 [
              "identificador" => "tblfn0005"
              "etiqueta" => "a"
              "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Serotypes 6B&#44; 14 and 19F are all present in PCV7&#44; PCV10 and PCV13&#46;</p> <p class="elsevierStyleNotepara" id="npar0010">PCV&#44; pneumococcal conjugate vaccine&#59; PCV7&#44; 7-valent PCV&#59; PCV10&#44; 10-valent PCV&#59; PCV13&#44; 13-valent PCV&#46;</p>"
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0105" class="elsevierStyleSimplePara elsevierViewall">Serogroups and serotypes of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span>-positive episodes &#40;by culture&#41; by name of pneumococcal conjugate vaccination status &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>16&#41;&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:29 [
            0 => array:3 [
              "identificador" => "bib0150"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Prospects for a vaccine against otitis media"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "A&#46;W&#46; Cripps"
                            1 => "D&#46;C&#46; Otczyk"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1586/14760584.5.4.517"
                      "Revista" => array:6 [
                        "tituloSerie" => "Expert Rev Vaccines"
                        "fecha" => "2006"
                        "volumen" => "5"
                        "paginaInicial" => "517"
                        "paginaFinal" => "534"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16989632"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0155"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The burden of otitis media"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "M&#46;M&#46; Rovers"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.vaccine.2008.11.005"
                      "Revista" => array:6 [
                        "tituloSerie" => "Vaccine"
                        "fecha" => "2008"
                        "volumen" => "26"
                        "paginaInicial" => "G2"
                        "paginaFinal" => "G4"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19094933"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0160"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Burden of disease caused by otitis media&#58; systematic review and global estimates"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "L&#46; Monasta"
                            1 => "L&#46; Ronfani"
                            2 => "F&#46; Marchetti"
                            3 => "M&#46; Montico"
                            4 => "L&#46; Vecchi Brumatti"
                            5 => "A&#46; Bavcar"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1371/journal.pone.0036226"
                      "Revista" => array:5 [
                        "tituloSerie" => "PLoS One"
                        "fecha" => "2012"
                        "volumen" => "7"
                        "paginaInicial" => "e36226"
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        "texto" => "<p id="par0160" class="elsevierStylePara elsevierViewall">The authors would like to thank Ashmita Ravishankar and Mark Franco for medical writing &#40;Publications Writer&#44; GSK&#41;&#44; Julia Donnelly &#40;freelance Publications Manager on behalf of GSK Vaccines&#41; for editorial support and J&#233;r&#233;mie Dedessus Le Moutier and Gr&#233;gory Leroux &#40;Publications Manager&#44; Business and Decision Life Sciences on behalf of GSK Vaccines&#41; for coordination in the development of the manuscript&#46;</p>"
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¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

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