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which moves as the patient breathes while the He&#44; CO and O<span class="elsevierStyleInf">2</span> concentrations are continuously monitored&#46; As the patient is connected to the system at functional residual capacity &#40;FRC&#41; level&#44; this FRC is determined by He dilution&#44; whereas diffusing capacity is calculated from the exponential decay in CO&#46; The results depend on alveolar ventilation&#44; and the breathing pattern must be as stable as possible&#46;<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a></p><p id="par0050" class="elsevierStylePara elsevierViewall">An inhalation bag with a two-way valve&#44; containing a mixture of CO at a certain concentration&#44; can also be used&#46; The patient breathes several times and the exhaled gas is collected and analysed&#46; The CO uptake is obtained by multiplying the difference between the inhaled and exhaled concentrations by the ventilation volume per minute&#46; The partial alveolar pressure of CO at which the transfer is made also needs to be measured&#46;<a class="elsevierStyleCrossRef" href="#bib0055"><span class="elsevierStyleSup">11</span></a></p><p id="par0055" class="elsevierStylePara elsevierViewall">There are predicted values for DLCO and DLCO&#47;VA with this technique for patients aged from 6 to 18&#46;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a></p><p id="par0060" class="elsevierStylePara elsevierViewall">It is to be expected that the use of new systems&#44; which analyse CO breath by breath&#44; not estimating its concentration but determining it in real time&#44; may avoid errors that arise with the other techniques&#46;</p><p id="par0065" class="elsevierStylePara elsevierViewall">Nevertheless&#44; new validation and standardisation studies of this technique are needed&#46;<ul class="elsevierStyleList" id="lis0010"><li class="elsevierStyleListItem" id="lsti0015"><span class="elsevierStyleLabel">&#40;c&#41;</span><p id="par0070" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Single-breath method</span> &#40;DLCOSB&#41;&#58; measuring DLCO per single breath involves making the patient exhale to residual volume &#40;RV&#41; and then take in a rapid breath of a gas containing CO 0&#46;3&#37;&#44; He 10&#37;&#44; O<span class="elsevierStyleInf">2</span> 21&#37; and nitrogen in equilibrium&#44; to over 90&#37; of vital capacity&#46; After rapid inhalation of the gas&#44; the breath is held for 10<span class="elsevierStyleHsp" style=""></span>s at total lung capacity &#40;TLC&#41; to allow it to diffuse and then the patient is made to exhale rapidly&#46; The first part &#40;between 0&#46;5 and 1<span class="elsevierStyleHsp" style=""></span>L&#41; is discarded&#44; as it corresponds to the dead space that has not undergone the diffusion process&#44; and the second&#44; representing the gas that has been in the alveoli &#40;alveolar fraction&#41;&#44; is used&#46; In this second fraction the final concentration of CO and He is determined&#46; The initial concentration of CO in the alveolar gas is not the same as the concentration of CO in the inhaled gas administered to the patient&#44; since this gas has to be diluted in the air present in the lung after maximum exhalation &#40;RV&#41; and air in the dead space in the system&#46; Since only the concentration of the gas administered is known and not that present in the alveolar space&#44; a tracer gas &#40;generally He&#41; is used to calculate the latter&#46; He is an inert gas&#44; which is not diffused through the alveolar-capillary membrane&#46; Therefore&#44; if we know the initial concentration of He in the gas administered &#40;He<span class="elsevierStyleInf"><span class="elsevierStyleItalic">I</span></span>&#41; and the volume of gas inhaled &#40;measured with the spirometer&#41;&#44; the final concentration of helium &#40;He<span class="elsevierStyleInf"><span class="elsevierStyleItalic">E</span></span>&#41;&#44; measured in the exhaled air&#44; will depend on the final volume in which the He has been dissolved &#40;alveolar volume<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>dead space&#41;&#46;</p></li></ul><elsevierMultimedia ident="eq0010"></elsevierMultimedia>Knowing this final volume we can easily calculate the initial alveolar CO concentration FACO<span class="elsevierStyleInf"><span class="elsevierStyleItalic">i</span></span>&#58;<elsevierMultimedia ident="eq0015"></elsevierMultimedia>Using He also enables us to calculate the alveolar volume &#40;VA&#41;&#44; which will be the volume of air in the alveolar space &#40;that which is diffused&#41;&#46;<elsevierMultimedia ident="eq0020"></elsevierMultimedia>The dead space depends both on the dead space in the system &#40;it must be less than 100<span class="elsevierStyleHsp" style=""></span>mL&#41;&#44; which we know&#44; and on the anatomical dead space &#40;2&#46;2<span class="elsevierStyleHsp" style=""></span>mL&#47;kg of body weight&#41;&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">The main criticism of this method is that it measures diffusion in a very unphysiological situation&#58; during a maximum inhalation and while the breath is held&#44; which does not occur in normal breathing at tidal volume&#46;</p><p id="par0080" class="elsevierStylePara elsevierViewall">Another of the problems with DLCO is that we are using a single value to express the diverse properties of millions of respiratory units&#44; which differ according to the region of the lung&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">Single-breath DLCO depends on the amount of lung tissue involved in the gas exchange&#46; For this reason it is advisable in these patients to assess diffusing capacity per unit of lung volume&#44; KCO &#40;DLCO&#47;VA&#41;&#46;<a class="elsevierStyleCrossRefs" href="#bib0065"><span class="elsevierStyleSup">13&#44;14</span></a></p><p id="par0090" class="elsevierStylePara elsevierViewall">The complexity of the technique&#44; the reference equations for the test&#44; differences in equipment&#44; interpatient variability and the conditions in which the test is performed mean that there is a high degree of interlaboratory variability&#44;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> much greater than with other pulmonary function measurements&#44; and standardisation is therefore essential&#46;<a class="elsevierStyleCrossRefs" href="#bib0010"><span class="elsevierStyleSup">2&#44;8&#44;16&#44;17</span></a> Reference values have been established in healthy white children aged from 5 to 19 for DLCO and VA&#46;<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">18</span></a></p><p id="par0095" class="elsevierStylePara elsevierViewall">We must not forget that the absolute values obtained with these two techniques&#44; single- and multiple-breath&#44; are different&#44; as they are performed at total lung capacity &#40;TLC&#41; and functional residual capacity &#40;FRC&#41; respectively&#44; and diffusion capacity is related to the surface area assessed&#44; which is lower in the latter&#59; therefore when the two techniques are compared the results have to be calculated as z-scores or standard deviations from the corresponding predicted values&#46;</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Practical aspects of the technique</span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Preparation of the patient</span><p id="par0100" class="elsevierStylePara elsevierViewall">The patient must refrain from smoking for 24<span class="elsevierStyleHsp" style=""></span>h before the test&#44; avoid alcohol for at least 4<span class="elsevierStyleHsp" style=""></span>h and not take exercise&#46; He or she will remain seated for at least 5<span class="elsevierStyleHsp" style=""></span>min before the test and throughout the procedure&#46;<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">19</span></a></p><p id="par0105" class="elsevierStylePara elsevierViewall">If the patient requires oxygen it is preferable to discontinue it at least 5<span class="elsevierStyleHsp" style=""></span>min before beginning the test&#46; If this is not possible&#44; the results must be assessed with caution&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Equipment</span><p id="par0110" class="elsevierStylePara elsevierViewall">The patient breathes through a pneumotacograph&#44; which measures the volume of air inhaled and is connected to a three-way valve&#44; which initially allows the patient to breathe ambient air&#59; subsequently&#44; during forced inhalation&#44; the way is opened to the gas cylinder&#44; and during exhalation it is opened to the bag in which the alveolar gas sample is collected&#46; During the 10<span class="elsevierStyleHsp" style=""></span>s for which the breath is held there must be a shutter that prevents exhalation and also a pressure sensor to assess whether the patient is performing Valsalva manoeuvres &#40;exhalation&#41; or M&#252;ller&#39;s manoeuvres &#40;inhalation&#41;&#44; which modify pulmonary capillary blood volume&#46; The Valsalva manoeuvre reduces DLCO and M&#252;ller&#39;s manoeuvre increases it&#46;<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">20</span></a> Finally&#44; gas analysers are needed to ascertain the concentration of exhaled CO and He&#46; The system must have a dead space of less than 100<span class="elsevierStyleHsp" style=""></span>mL&#46; All the apparatuses must be calibrated daily&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a></p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Test procedure</span><p id="par0115" class="elsevierStylePara elsevierViewall"><ul class="elsevierStyleList" id="lis0015"><li class="elsevierStyleListItem" id="lsti0020"><span class="elsevierStyleLabel">1&#41;</span><p id="par0120" class="elsevierStylePara elsevierViewall">Explain the manoeuvre&#46; It is advisable to carry out a simulation first without gas inhalation&#46;</p></li><li class="elsevierStyleListItem" id="lsti0025"><span class="elsevierStyleLabel">2&#41;</span><p id="par0125" class="elsevierStylePara elsevierViewall">The mouthpiece is placed in the mouth and the clip on the nose and the patient is asked to breathe calmly&#46;</p></li><li class="elsevierStyleListItem" id="lsti0030"><span class="elsevierStyleLabel">3&#41;</span><p id="par0130" class="elsevierStylePara elsevierViewall">The patient is told to exhale to RV&#46; If there is a major obstructive disease it is recommended that exhalation should be limited to 6<span class="elsevierStyleHsp" style=""></span>s&#46;</p></li><li class="elsevierStyleListItem" id="lsti0035"><span class="elsevierStyleLabel">4&#41;</span><p id="par0135" class="elsevierStylePara elsevierViewall">A rapid breath is taken to TLC&#58;<ul class="elsevierStyleList" id="lis0020"><li class="elsevierStyleListItem" id="lsti0040"><span class="elsevierStyleLabel">a&#41;</span><p id="par0140" class="elsevierStylePara elsevierViewall">The inspiratory volume &#40;IV&#41; must be at least 90&#37; of the largest previous VC &#40;spirometry must be performed beforehand as a guide&#41;&#46;</p></li><li class="elsevierStyleListItem" id="lsti0045"><span class="elsevierStyleLabel">b&#41;</span><p id="par0145" class="elsevierStylePara elsevierViewall">The inspiration must be rapid enough for 90&#37; of the IV to be inhaled within 1&#46;5&#8211;2<span class="elsevierStyleHsp" style=""></span>s in healthy individuals and in less than 4<span class="elsevierStyleHsp" style=""></span>s in patients with obstructive disease&#46; If the inhalation lasts for 5<span class="elsevierStyleHsp" style=""></span>s&#44; DLCO increases by around 13&#37;&#46;</p></li></ul></p></li><li class="elsevierStyleListItem" id="lsti0050"><span class="elsevierStyleLabel">5&#41;</span><p id="par0150" class="elsevierStylePara elsevierViewall">The breath must be held for 10<span class="elsevierStyleHsp" style=""></span>s&#46;<ul class="elsevierStyleList" id="lis0025"><li class="elsevierStyleListItem" id="lsti0055"><span class="elsevierStyleLabel">a&#41;</span><p id="par0155" class="elsevierStylePara elsevierViewall">During this time no expiratory or inspiratory effort must be made against the shutter&#46;</p></li></ul></p></li><li class="elsevierStyleListItem" id="lsti0060"><span class="elsevierStyleLabel">6&#41;</span><p id="par0160" class="elsevierStylePara elsevierViewall">After this the patient must exhale rapidly&#46;<ul class="elsevierStyleList" id="lis0030"><li class="elsevierStyleListItem" id="lsti0065"><span class="elsevierStyleLabel">a&#41;</span><p id="par0165" class="elsevierStylePara elsevierViewall">The first part &#40;about 750<span class="elsevierStyleHsp" style=""></span>mL&#41; is discarded&#46; If the patient has a VC<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>2<span class="elsevierStyleHsp" style=""></span>L this can be reduced to 500<span class="elsevierStyleHsp" style=""></span>mL&#46;</p></li><li class="elsevierStyleListItem" id="lsti0070"><span class="elsevierStyleLabel">b&#41;</span><p id="par0170" class="elsevierStylePara elsevierViewall">The volume analysed must be between 500 and 1<span class="elsevierStyleHsp" style=""></span>000<span class="elsevierStyleHsp" style=""></span>mL and must be exhaled in less than 4<span class="elsevierStyleHsp" style=""></span>s&#46;</p></li></ul></p></li><li class="elsevierStyleListItem" id="lsti0075"><span class="elsevierStyleLabel">7&#41;</span><p id="par0175" class="elsevierStylePara elsevierViewall">The patient is told to remove the nose clip and remain seated&#46;</p></li><li class="elsevierStyleListItem" id="lsti0080"><span class="elsevierStyleLabel">8&#41;</span><p id="par0180" class="elsevierStylePara elsevierViewall">The test must be repeated until at least two values are achieved that agree within 10&#37; and below 3<span class="elsevierStyleHsp" style=""></span>mL&#47;min&#47;mmHg&#46; At least 4<span class="elsevierStyleHsp" style=""></span>min must elapse between each test&#46; In patients with major obstruction it may be necessary to wait up to 10<span class="elsevierStyleHsp" style=""></span>min between tests for the gases to be completely eliminated&#46; It may be useful to ask the patient to take deep breaths so as to eliminate the gas more effectively&#46;</p></li><li class="elsevierStyleListItem" id="lsti0085"><span class="elsevierStyleLabel">9&#41;</span><p id="par0185" class="elsevierStylePara elsevierViewall">Calculations&#58;</p></li></ul><elsevierMultimedia ident="eq0025"></elsevierMultimedia>VA<span class="elsevierStyleInf">STPD</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>alveolar volume at STPD&#59; 60&#47;<span class="elsevierStyleItalic">t</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>breath-hold time converted into minutes&#59; PB&#8211;47<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>barometric pressure minus water vapour pressure at 37<span class="elsevierStyleHsp" style=""></span>&#176;C&#59; ln<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>natural logarithm&#59; FA<span class="elsevierStyleSmallCaps">CO</span><span class="elsevierStyleInf">o</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>alveolar CO concentration at the start of diffusion&#59; FACO<span class="elsevierStyleInf"><span class="elsevierStyleItalic">t</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>alveolar CO concentration at the end of the test&#46;<elsevierMultimedia ident="eq0030"></elsevierMultimedia>He<span class="elsevierStyleInf"><span class="elsevierStyleItalic">E</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>exhaled He concentration&#59; He<span class="elsevierStyleInf"><span class="elsevierStyleItalic">I</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>inhaled He concentration&#59; CO<span class="elsevierStyleInf"><span class="elsevierStyleItalic">I</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>inhaled CO concentration&#59; CO<span class="elsevierStyleInf"><span class="elsevierStyleItalic">E</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>exhaled CO concentration&#46;</p><p id="par0190" class="elsevierStylePara elsevierViewall">Given that diffusing capacity is calculated per unit of time &#40;mL of CO absorbed per minute&#41;&#44; exact calculation of the time the patient holds his or her breath&#44; which is the time during which CO diffusion takes place&#44; is crucial&#46;<a class="elsevierStyleCrossRefs" href="#bib0110"><span class="elsevierStyleSup">22&#44;23</span></a> The Jones&#8211;Meade method for measuring the time starts counting from 30&#37; of the inhalation time and stops at 50&#37; of the alveolar gas sample collection time &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><p id="par0195" class="elsevierStylePara elsevierViewall">In patients with obstruction it may be preferable to perform the test after using a bronchodilator&#44; although if heart rate increases&#44; volume per minute also increases&#44; raising DLCO&#46; For this reason it is advisable to carry out the test at least 30<span class="elsevierStyleHsp" style=""></span>min after administering the bronchodilator&#46;</p></span></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Adjustments</span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Adjustment for haemoglobin &#40;Hb&#41;</span><p id="par0200" class="elsevierStylePara elsevierViewall">Patients with anaemia have a reduced DLCO because CO uptake by erythrocytes is lower&#46; The Cotes method<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a> of correction for Hb makes it possible to calculate adjusted DLCO in adult males with Hb greater than 14&#46;6<span class="elsevierStyleHsp" style=""></span>g&#47;dL or in women and in children under 15 with Hb<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>13&#46;4<span class="elsevierStyleHsp" style=""></span>g&#47;dL&#46;</p><p id="par0205" class="elsevierStylePara elsevierViewall">Males 15 years of age and older&#58;<elsevierMultimedia ident="eq0035"></elsevierMultimedia>Children aged &#60;15 years and women&#58;<elsevierMultimedia ident="eq0040"></elsevierMultimedia>It is advisable to put both values in the report&#58; the measured DLCO and the DLCO adjusted for Hb&#46;</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Clinical applications</span><p id="par0210" class="elsevierStylePara elsevierViewall">CO transfer can be either increased or reduced in various processes &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">8</span></a></p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0215" class="elsevierStylePara elsevierViewall">CO transfer is increased in situations in which there is an increase in blood volume in the pulmonary capillaries &#40;exercise&#44; left-right shunts&#41;&#44; polycythaemia and pulmonary haemorrhage &#40;falsely elevated DLCO&#41;&#46; Occasionally there is a rise in DLCO in asthmatics&#44; due to an increase in pulmonary blood volume though negative intrathoracic pressure produced after rapid inhalations&#46;</p><p id="par0220" class="elsevierStylePara elsevierViewall">DLCO is decreased in patients with a reduction in alveolar volume or in diffusion defects &#40;<span class="elsevierStyleItalic">alteration of the alveolar-capillary membrane</span> &#91;interstitial lung disease&#58; idiopathic pulmonary fibrosis&#44; extrinsic allergic alveolitis&#44; scleroderma&#44; sarcoidosis&#44; asbestosis&#93; or <span class="elsevierStyleItalic">decreased pulmonary capillary blood volume</span> &#91;pulmonary embolism or primary pulmonary hypertension&#93;&#41;&#46; In pulmonary emphysema DLCO is characteristically reduced by loss of alveolar-capillary membrane surface area secondary to alveolar rupture&#44; giving rise to an increase in TLC with a decreased KCO &#40;DLCO&#47;VA&#41;&#46; In congestive heart failure the reduction in DLCO may be secondary to interstitial oedema&#46; Other causes of decreased DLCO are anaemia&#44; renal failure&#44; smoking and marijuana use&#46;</p><p id="par0225" class="elsevierStylePara elsevierViewall">In patients with restrictive diseases&#44; especially in children with deformities of the rib cage or neuromuscular diseases in which normal lungs are restrictive because of deformity or muscular weakness&#44; the surface area for diffusion is relatively large per unit of pulmonary volume&#44; and for this reason DLCO may be normal&#46; The patient&#39;s DLCO&#47;VA should therefore be compared with reference values based on his or her &#40;restrictive&#41; TLC rather than his or her theoretical TLC&#46;<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">25</span></a></p><p id="par0230" class="elsevierStylePara elsevierViewall">In cystic fibrosis both raising and lowering of DLCO may be found&#46; Initially the transfer factor may be elevated by an increase in the amount of blood reaching the lung&#44; due to fluctuations in pleural pressure secondary to bronchial obstruction&#59; later&#44; with the onset of pulmonary heart disease&#44; pulmonary microcirculation is impaired and diffusion is gradually reduced&#46;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a></p><p id="par0235" class="elsevierStylePara elsevierViewall">Indications for the DLCO test are set out in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a></p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0240" class="elsevierStylePara elsevierViewall">In practice the main indications in paediatrics are as follows<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a>&#58;</p><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Monitoring of treatments that are toxic to the lungs</span><p id="par0245" class="elsevierStylePara elsevierViewall">Chemotherapy &#40;methotrexate&#44; nitrofurantoin&#44; azathioprine&#44; penicillamine&#44; cyclophosphamide and especially bleomycin&#41; can produce a major decrease in DLCO&#44; which must be monitored&#46;</p><p id="par0250" class="elsevierStylePara elsevierViewall">Chest radiation can cause irreversible diffusion impairment&#46;</p><p id="par0255" class="elsevierStylePara elsevierViewall">Treatments for autoimmune or rheumatological diseases can also impair pulmonary diffusion&#46;</p><p id="par0260" class="elsevierStylePara elsevierViewall">Immunosuppressants used after organ transplants have been associated with obstructive and restrictive lung disease&#46;</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Diagnosis and monitoring of patients with chronic interstitial lung disease</span><p id="par0265" class="elsevierStylePara elsevierViewall">Impairment of diffusing capacity is one of the initial signs of interstitial lung disease and a fundamental indicator of clinical course and response to treatment&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Monitoring of children with diseases that cause pulmonary bleeding</span><p id="par0270" class="elsevierStylePara elsevierViewall">In primary pulmonary haemosiderosis&#44; Goodpasture syndrome and granulomatosis with polyangiitis&#44; an increase in diffusing capacity can predict relapse or indicate progression in asymptomatic patients&#46; Moreover&#44; pulmonary haemosiderosis that does not respond to treatment may eventually give rise to pulmonary fibrosis&#44; leading to decreased diffusion&#46;</p></span></span></span>"
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        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">The diffusion capacity is the technique that measures the ability of the respiratory system for gas exchange&#44; thus allowing a diagnosis of the malfunction of the alveolar-capillary unit&#46; The most important parameter to assess is the CO diffusion capacity &#40;DLCO&#41;&#46; New methods are currently being used to measure the diffusion using nitric oxide &#40;NO&#41;&#46; There are other methods for measuring diffusion&#44; although in this article the single breath technique is mainly referred to&#44; as it is the most widely used and best standardised&#46;</p><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Its complexity&#44; its reference equations&#44; differences in equipment&#44; inter-patient variability and conditions in which the DLCO is performed&#44; lead to a wide inter-laboratory variability&#44; although its standardisation makes this a more reliable and reproductive method&#46;</p><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The practical aspects of the technique are analysed&#44; by specifying the recommendations to carry out a suitable procedure&#44; the calibration routine&#44; calculations and adjustments&#46; Clinical applications are also discussed&#46; An increase in the transfer of CO occurs in diseases in which there is an increased volume of blood in the pulmonary capillaries&#44; such as in polycythemia and pulmonary haemorrhage&#46; There is a decrease in DLCO in patients with alveolar volume reduction or diffusion defects&#44; either by altered alveolar-capillary membrane &#40;interstitial diseases&#41; or decreased volume of blood in the pulmonary capillaries &#40;pulmonary embolism or primary pulmonary hypertension&#41;&#46; Other causes of decreased or increased DLCO are also highlighted&#46;</p></span>"
      ]
      "es" => array:2 [
        "titulo" => "Resumen"
        "resumen" => "<span id="abst0010" class="elsevierStyleSection elsevierViewall"><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">La capacidad de difusi&#243;n es la t&#233;cnica que mide la capacidad del aparato respiratorio para realizar el intercambio gaseoso y as&#237; diagnosticar la disfunci&#243;n de la unidad alv&#233;olo-capilar&#46;</p><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">El par&#225;metro m&#225;s importante a evaluar es la capacidad de difusi&#243;n del CO &#40;DLCO&#41;&#46; Actualmente hay nuevos m&#233;todos para medir la capacidad de difusi&#243;n utilizando &#243;xido n&#237;trico &#40;NO&#41;&#46; Existen diferentes m&#233;todos de medida&#44; aunque en este art&#237;culo nos referiremos sobre todo a la t&#233;cnica de la respiraci&#243;n &#250;nica&#44; la m&#225;s utilizada y mejor estandarizada&#46;</p><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Su complejidad&#44; sus ecuaciones de referencia&#44; las diferencias en equipamiento&#44; la variabilidad interpacientes y las condiciones en las que se realiza hacen que exista una gran variabilidad interlaboratorio&#44; habi&#233;ndose realizado estandarizaciones para hacer este m&#233;todo m&#225;s fiable y reproducible&#46;</p><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Se analizan los aspectos pr&#225;cticos de la t&#233;cnica&#44; especificando las recomendaciones para la realizaci&#243;n de un procedimiento adecuado&#44; sistem&#225;tica de calibraci&#243;n y c&#225;lculos y ajustes necesarios&#46; Tambi&#233;n se exponen sus aplicaciones cl&#237;nicas&#46;</p><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Se produce un aumento de la transferencia de CO en las enfermedades en las que existe un aumento del volumen sangu&#237;neo en los capilares pulmonares&#44; en la policitemia y en la hemorragia pulmonar&#46; Existe una disminuci&#243;n de la DLCO en los pacientes con reducci&#243;n del volumen alveolar o en los defectos de difusi&#243;n&#44; ya sea por alteraci&#243;n de la membrana alv&#233;olo-capilar &#40;enfermedad intersticial&#41; o por disminuci&#243;n del volumen de sangre en los capilares pulmonares &#40;embolia pulmonar o hipertensi&#243;n pulmonar primaria&#41;&#46; Tambi&#233;n se exponen otras causas de disminuci&#243;n o aumento de la DLCO&#46;</p></span>"
      ]
    ]
    "NotaPie" => array:1 [
      0 => array:2 [
        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Please cite this article as&#58; Posadas AS&#44; Asensi JRV&#44; de MirMessa I&#44; Prado OS&#44; Larramona H&#46; Medici&#243;n de la difusi&#243;n de CO &#40;II&#41;&#58; estandarizaci&#243;n y criterios de calidad&#46; An Pediatr &#40;Barc&#41;&#46; 2015&#59;83&#58;137&#46;e1&#8211;137&#46;e7&#46;</p>"
      ]
    ]
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Structure of the gas exchange barrier&#46;</p>"
        ]
      ]
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          "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Graphic illustration of the Ogilvie and Jones&#8211;Meade methods for calculating breath-hold time during the DLCO manoeuvre&#46;</p>"
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        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
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                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Extrapulmonary reduction in alveolar volume</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Reduced respiratory effort or muscular debility&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Thoracic deformities preventing full inflation of the thorax&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Situations involving reduction of the CO-Hb reaction</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Anaemia&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary embolism&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Changes in haemoglobin&#39;s binding properties &#40;carboxyhaemoglobin&#44; increased FiO2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Conditions that alter the alveolar-capillary membrane and the CO-Hb binding&#44; reducing DLCO</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary resection &#40;there may be a compensating increase in the CO-Hb reaction&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Emphysema&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Interstitial lung disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary oedema&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary vasculitis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary hypertension&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Situations that increase the CO-Hb reaction and raise DLCO</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Polycythaemia&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Left-right shunt&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary haemorrhage&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Asthma&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Changes in the Hb binding properties &#40;reduced FiO2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>M&#252;ller&#39;s manoeuvre &#40;reduction in intrathoracic pressure&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Exercise&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Supine position&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Obesity&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
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          "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Processes associated with alterations in DLCO&#46;</p>"
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                  \t\t\t\t" class=""><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">1&#46; Evaluation and followup of diseases affecting lung parenchyma &#40;those associated with drug reactions&#44; pneumoconiosis or sarcoidosis&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">2&#46; Evaluation and followup of emphysema&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">3&#46; Differentiating between chronic bronchitis&#44; emphysema and asthma&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">4&#46; Assessment of pulmonary involvement in systemic diseases&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">5&#46; Assessment of cardiovascular diseases&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">6&#46; Prediction of arterial desaturation during exercise in some patients with lung disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">7&#46; Assessment and quantification of the degree of disability associated with pulmonary fibrosis or with emphysema&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">8&#46; Assessment of the pulmonary effects of chemotherapy agents or other drugs that induce pulmonary dysfunction&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">9&#46; Assessment of pulmonary haemorrhage&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">10&#46; As an early indicator of certain pulmonary infections that cause diffuse pneumonitis &#40;e&#46;g&#46;&#44; <span class="elsevierStyleItalic">Pneumocystis</span> pneumonia&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
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          "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Indications for DLCO measurement&#46;</p>"
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    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:26 [
            0 => array:3 [
              "identificador" => "bib0005"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Single-breath carbon monoxide diffusing capacity"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "J&#46; Wanger"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:4 [
                        "titulo" => "Pulmonary function testing"
                        "paginaInicial" => "145"
                        "paginaFinal" => "173"
                        "serieFecha" => "1996"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0010"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Standardisation of the single-breath determination of carbon monoxide uptake in the lung"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "N&#46; MacIntyre"
                            1 => "R&#46;O&#46; Crapo"
                            2 => "G&#46; Viegi"
                            3 => "D&#46;C&#46; Johnson"
                            4 => "C&#46;P&#46; van der Grinten"
                            5 => "V&#46; Brusasco"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1183/09031936.05.00034905"
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur Respir J"
                        "fecha" => "2005"
                        "volumen" => "26"
                        "paginaInicial" => "720"
                        "paginaFinal" => "735"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16204605"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0015"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Diffusing capacity for nitric oxide&#58; reference values and dependence on alveolar volume"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "I&#46; Van der Lee"
                            1 => "P&#46; Zanen"
                            2 => "N&#46; Stigter"
                            3 => "J&#46;M&#46; van den Bosch"
                            4 => "J&#46;W&#46; Lammers"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.rmed.2006.12.001"
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                        "tituloSerie" => "Respir Med"
                        "fecha" => "2007"
                        "volumen" => "101"
                        "paginaInicial" => "1579"
                        "paginaFinal" => "1584"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17229562"
                            "web" => "Medline"
                          ]
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              "identificador" => "bib0020"
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                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "The TLNO&#47;TLCO ratio in pulmonary function test interpretation"
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                          "autores" => array:2 [
                            0 => "J&#46;M&#46; Hughes"
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                      "doi" => "10.1183/09031936.00082112"
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                        "tituloSerie" => "Eur Respir J"
                        "fecha" => "2013"
                        "volumen" => "41"
                        "paginaInicial" => "453"
                        "paginaFinal" => "461"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22936707"
                            "web" => "Medline"
                          ]
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              "identificador" => "bib0025"
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                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "Lung diffusing capacity for nitric oxide and carbon monoxide in relation to morphological changes as assessed by computed tomography in patients with cystic fibrosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
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                            1 => "L&#46; Filser"
                            2 => "R&#46; Fischer"
                            3 => "K&#46; Marten"
                            4 => "U&#46; M&#252;ller-Lisse"
                            5 => "D&#46; de la Motte"
                          ]
                        ]
                      ]
                    ]
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                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1186/1471-2466-9-30"
                      "Revista" => array:5 [
                        "tituloSerie" => "BMC Pulm Med"
                        "fecha" => "2009"
                        "volumen" => "9"
                        "paginaInicial" => "30"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19531222"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
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            5 => array:3 [
              "identificador" => "bib0030"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lung diffusing capacity for nitric oxide and carbon monoxide&#58; dependence on breath-hold time"
                      "autores" => array:1 [
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                          "etal" => true
                          "autores" => array:6 [
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                            1 => "L&#46; Filser"
                            2 => "R&#46; Fischer"
                            3 => "D&#46; de la Motte"
                            4 => "W&#46; Steinhaeusser"
                            5 => "R&#46;M&#46; Huber"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1378/chest.07-2388"
                      "Revista" => array:6 [
                        "tituloSerie" => "Chest"
                        "fecha" => "2008"
                        "volumen" => "133"
                        "paginaInicial" => "1149"
                        "paginaFinal" => "1154"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18263682"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0035"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "European reference equations for CO and NO lung transfer"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "B&#46; Aguilaniu"
                            1 => "J&#46; Maitre"
                            2 => "S&#46; Gle¿net"
                            3 => "A&#46; Gegout-Petit"
                            4 => "H&#46; Gue¿nard"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1183/09031936.00063207"
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur Respir J"
                        "fecha" => "2008"
                        "volumen" => "31"
                        "paginaInicial" => "1091"
                        "paginaFinal" => "1097"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18216061"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0040"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Single-breath carbon monoxide diffusing capacity &#40;transfer factor&#41;&#46; Recommendations for a standard technique&#58; 1995 update"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "American Thoracic Society"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/ajrccm.152.6.8520796"
                      "Revista" => array:8 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "1995"
                        "volumen" => "152"
                        "numero" => "6 Pt 1"
                        "paginaInicial" => "2185"
                        "paginaFinal" => "2198"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8520796"
                            "web" => "Medline"
                          ]
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                          "pii" => "S0002937804005381"
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                          "issn" => "00029378"
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                  ]
                ]
              ]
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            8 => array:3 [
              "identificador" => "bib0045"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Comparison of carbon monoxide &#40;CO&#41; single breath pulmonary diffusing capacity with non-rebreathing&#44; open-circuit CO pulmonary diffusing capacity in healthy children"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "O&#46;E&#46; Suman"
                            1 => "S&#46; Thomas"
                            2 => "K&#46;C&#46; Beck"
                            3 => "R&#46;P&#46; Mlcak"
                            4 => "D&#46;N&#46; Herndon"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1002/ppul.20504"
                      "Revista" => array:6 [
                        "tituloSerie" => "Pediatr Pulmonol"
                        "fecha" => "2006"
                        "volumen" => "41"
                        "paginaInicial" => "1095"
                        "paginaFinal" => "1102"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16986167"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0050"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lung diffusing capacity"
                      "autores" => array:1 [
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                          "autores" => array:3 [
                            0 => "J&#46;C&#46; de Jongste"
                            1 => "P&#46;J&#46;F&#46;M&#46; Merkus"
                            2 => "H&#46; Stam"
                          ]
                        ]
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                        "paginaFinal" => "165"
                        "serieTitulo" => "Paediatric pulmonary function testing&#46; Vol&#46; 33&#46; Prog Respir Res"
                        "serieFecha" => "2005"
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                  ]
                ]
              ]
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            10 => array:3 [
              "identificador" => "bib0055"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "Capacidad de difusi&#243;n"
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                            0 => "N&#46; Gonz&#225;lez Mangado"
                            1 => "M&#46;J&#46; Rodr&#237;guez Nieto"
                            2 => "J&#46; G&#243;mez Seco"
                          ]
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                      "LibroEditado" => array:4 [
                        "titulo" => "Exploraci&#243;n funcional respiratoria&#46; Monograf&#237;as Neumo&#46; Vol XVIII&#47;2011"
                        "paginaInicial" => "45"
                        "paginaFinal" => "58"
                        "serieFecha" => "2011"
                      ]
                    ]
                  ]
                ]
              ]
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            11 => array:3 [
              "identificador" => "bib0060"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "A rebreathing method to determine carbon monoxide diffusing capacity in children&#58; reference values for 6- to 18-year-olds &#91;corrected&#93; and validation in adult volunteers"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "H&#46; Stam"
                            1 => "A&#46;V&#46; Beek"
                            2 => "K&#46; Gr&#252;nberg"
                            3 => "M&#46;A&#46; de Ridder"
                            4 => "J&#46;C&#46; de Jongste"
                            5 => "A&#46; Versprille"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Pediatr Pulmonol"
                        "fecha" => "1998"
                        "volumen" => "25"
                        "paginaInicial" => "205"
                        "paginaFinal" => "212"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9556013"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0065"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pulmonary gas exchange"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "G&#46;J&#46; Gibson"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:5 [
                        "titulo" => "Clinical tests of respiratory function"
                        "paginaInicial" => "53"
                        "paginaFinal" => "75"
                        "edicion" => "3rd ed&#46;"
                        "serieFecha" => "2009"
                      ]
                    ]
                  ]
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              ]
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              "identificador" => "bib0070"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "Examination of the carbon monoxide diffusing capacity &#40;DL&#40;CO&#41;&#41; in relation to its KCO and VA components"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
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                            1 => "N&#46;B&#46; Pride"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/rccm.201112-2160CI"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2012"
                        "volumen" => "186"
                        "paginaInicial" => "132"
                        "paginaFinal" => "139"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22538804"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
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            14 => array:3 [
              "identificador" => "bib0075"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Facing the noise&#58; addressing the endemic variability in D&#40;LCO&#41; testing"
                      "autores" => array:1 [
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                          "etal" => false
                          "autores" => array:1 [
                            0 => "M&#46;C&#46; McCormack"
                          ]
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                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.4187/respcare.01435"
                      "Revista" => array:6 [
                        "tituloSerie" => "Respir Care"
                        "fecha" => "2012"
                        "volumen" => "57"
                        "paginaInicial" => "17"
                        "paginaFinal" => "23"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22222122"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
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            15 => array:3 [
              "identificador" => "bib0080"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Standardization of the measurement of transfer factor"
                      "autores" => array:1 [
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                          "etal" => false
                          "autores" => array:1 [
                            0 => "D&#46; Cramer"
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                        ]
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                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur Respir J"
                        "fecha" => "1993"
                        "volumen" => "6"
                        "paginaInicial" => "1577"
                        "paginaFinal" => "1578"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8112455"
                            "web" => "Medline"
                          ]
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              "identificador" => "bib0085"
              "etiqueta" => "17"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:1 [
                      "titulo" => "Guidelines for the measurement of respiratory function&#46; Recommendations of the British Thoracic Society and the Association of Respiratory Technicians and Physiologists"
                    ]
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                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "Respir Med"
                        "fecha" => "1994"
                        "volumen" => "88"
                        "numero" => "3"
                        "paginaInicial" => "165"
                        "paginaFinal" => "194"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8209067"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
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              "identificador" => "bib0090"
              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pulmonary diffusing capacity in healthy Caucasian children"
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                          "etal" => true
                          "autores" => array:6 [
                            0 => "Y&#46;J&#46; Kim"
                            1 => "G&#46;L&#46; Hall"
                            2 => "K&#46; Christoph"
                            3 => "R&#46; Tabbey"
                            4 => "Z&#46; Yu"
                            5 => "R&#46;S&#46; Tepper"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1002/ppul.21564"
                      "Revista" => array:6 [
                        "tituloSerie" => "Pediatr Pulmonol"
                        "fecha" => "2012"
                        "volumen" => "47"
                        "paginaInicial" => "469"
                        "paginaFinal" => "475"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22081537"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
            ]
            18 => array:3 [
              "identificador" => "bib0095"
              "etiqueta" => "19"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Normal values for single exhalation diffusing capacity and pulmonary capillary blood flow in sitting&#44; supine positions&#44; and during mild exercise"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "Y&#46;C&#46; Huang"
                            1 => "M&#46;J&#46; Helms"
                            2 => "N&#46;R&#46; MacIntyre"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Chest"
                        "fecha" => "1994"
                        "volumen" => "105"
                        "paginaInicial" => "501"
                        "paginaFinal" => "508"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8306754"
                            "web" => "Medline"
                          ]
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                    ]
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                ]
              ]
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            19 => array:3 [
              "identificador" => "bib0100"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effect of high negative inspiratory pressure on single breath CO diffusing capacity"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "D&#46;J&#46; Cotton"
                            1 => "J&#46;T&#46; Mink"
                            2 => "B&#46;L&#46; Graham"
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                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Respir Physiol"
                        "fecha" => "1983"
                        "volumen" => "54"
                        "paginaInicial" => "19"
                        "paginaFinal" => "29"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/6648064"
                            "web" => "Medline"
                          ]
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                      ]
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                  ]
                ]
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            ]
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              "identificador" => "bib0105"
              "etiqueta" => "21"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Single-breath carbon monoxide diffusing capacity &#40;DLCO&#41; or transfer factor"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
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                          ]
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                      "LibroEditado" => array:2 [
                        "titulo" => "Pulmonary function laboratory mangement and procedure manual&#46; A project of the American Thoracic Society"
                        "serieFecha" => "1998"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib0110"
              "etiqueta" => "22"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "A standardized breath holding technique for the clinical measurement of the diffusing capacity of the lung for carbon monoxide"
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                          "autores" => array:4 [
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                            2 => "J&#46;W&#46; Morton"
                            3 => "C&#46;M&#46; Ogilvie"
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                        ]
                      ]
                    ]
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                    0 => array:2 [
                      "doi" => "10.1172/JCI103405"
                      "Revista" => array:7 [
                        "tituloSerie" => "J Clin Invest"
                        "fecha" => "1957"
                        "volumen" => "36"
                        "numero" => "1 Part 1"
                        "paginaInicial" => "1"
                        "paginaFinal" => "17"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16695816"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
            ]
            22 => array:3 [
              "identificador" => "bib0115"
              "etiqueta" => "23"
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                      "titulo" => "Reduction of the single breath CO diffusing capacity in cystic fibrosis"
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Spanish Association of Paediatrics
Measurement of CO diffusion capacity (II): Standardisation and quality criteria
Medición de la difusión de CO (II): estandarización y criterios de calidad
A. Salcedo-Posadasa, J.R. Villa-Asensib, I. de Mir Messac,
Corresponding author
, O. Sardon-Pradod,e, H. Larramonaf
a Sección de Neumología, Hospital Maternoinfantil Gregorio Marañón, Madrid, Spain
b Sección de Neumología, Hospital Infantil Universitario Niño Jesús, Madrid, Spain
c Sección de Neumología Pediátrica y Fibrosis Quística, Hospital Universitario Vall d¿Hebron, Barcelona, Spain
d Sección de Neumología Pediátrica, Hospital Universitario Donostia, San Sebastián, Spain
e Departamento de Pediatría, Facultad de Medicina y Odontología, UPV/EHU, San Sebastián, Spain
f Sección de Neumología Pediátrica, Consorci Hospitalari Parc Taulí, Sabadell, Barcelona, Spain
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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">The main function of the lungs is to establish the exchange of oxygen &#40;O<span class="elsevierStyleInf">2</span>&#41; and carbon dioxide &#40;CO<span class="elsevierStyleInf">2</span>&#41; between tissue and ambient air&#46; Gas exchange depends on three processes&#58; ventilation&#44; diffusion and pulmonary perfusion&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall">The diffusion process is defined as the flow of particles from an area of greater concentration to an area of lesser concentration&#46; The rate of transfer by diffusion of any gas through a membrane &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41; is directly proportional to the surface area of the membrane and inversely proportional to its thickness&#59; it also depends on the molecular weight&#44; pressure gradient and solubility of the gas&#44; the ventilation-perfusion ratio of the lung units&#44; the rate of combination with haemoglobin and the haemoglobin values&#46; The diffusion rate of CO<span class="elsevierStyleInf">2</span> is 20 times higher than that of O<span class="elsevierStyleInf">2</span>&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0015" class="elsevierStylePara elsevierViewall">Measuring diffusion provides information on gas transfer between the alveoli and the blood of the pulmonary capillaries and we generally refer to it as diffusion capacity &#40;DLCO&#41;&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#44;2</span></a> To assess the functional integrity of the diffusion process a gas must be used that is not present in venous blood&#44; that has an affinity for haemoglobin and that is soluble in blood&#46; The gas universally used is carbon monoxide &#40;CO&#41;&#46;</p><p id="par0020" class="elsevierStylePara elsevierViewall">CO diffusing capacity &#40;DLCO&#41; is the uptake of this gas per minute &#40;VCO&#58; mL of CO transferred per minute&#41; in relation to the CO gradient through alveolar-capillary membrane &#40;the difference between the partial pressures of CO in the alveoli &#91;PACO&#93; and in the capillary blood &#91;PCCO&#93; in mmHg&#41;&#46;<elsevierMultimedia ident="eq0005"></elsevierMultimedia></p><p id="par0025" class="elsevierStylePara elsevierViewall">There are currently new methods for measuring diffusion capacity using nitric oxide &#40;NO&#41;&#44; as this gas is independent of O<span class="elsevierStyleInf">2</span> pressure and haematocrit and has a greater affinity for haemoglobin &#40;Hb&#41; than CO&#46; Moreover&#44; DLNO is less influenced by capillary blood volume than DLCO and represents the true diffusing capacity of the alveolar-capillary membrane&#46; In addition&#44; the DLNO&#47;DLCO ratio could be a good indicator of impairment in gas exchange&#44; which would be an improvement on the tests currently used in clinical practice&#46;<a class="elsevierStyleCrossRefs" href="#bib0015"><span class="elsevierStyleSup">3&#44;4</span></a> In cystic fibrosis&#44; combined analysis with CO and NO could improve its functional assessment&#46;<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> Research is also being conducted on the influence of breath-hold time on diffusion capacity of NO compared with CO&#46;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> The use of NO is therefore a promising method&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a></p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Techniques for measuring diffusion</span><p id="par0030" class="elsevierStylePara elsevierViewall">There are three main methods for measuring DLCO&#58; &#40;a&#41; the <span class="elsevierStyleItalic">rebreathing</span> technique&#44; &#40;b&#41; the multiple-breath or <span class="elsevierStyleItalic">steady state</span> technique&#44; and &#40;c&#41; the <span class="elsevierStyleItalic">single-breath</span> &#40;SB&#41; technique&#46; The most widely used and best standardised method is the last of these&#46;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">8</span></a><ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005"><span class="elsevierStyleLabel">&#40;a&#41;</span><p id="par0035" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Rebreathing method</span>&#58; the patient breathes for 10&#8211;15<span class="elsevierStyleHsp" style=""></span>s into a small bag containing CO and helium &#40;He&#41;&#46; Little used in clinical practice&#46;</p></li><li class="elsevierStyleListItem" id="lsti0010"><span class="elsevierStyleLabel">&#40;b&#41;</span><p id="par0040" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Multiple-breath technique</span>&#58; many children have difficulties in holding their breath for 10<span class="elsevierStyleHsp" style=""></span>s or have a vital capacity &#40;VC&#41; lower than 1&#46;5<span class="elsevierStyleHsp" style=""></span>L&#44; so that they cannot perform the single-breath technique&#59; other techniques have therefore been devised&#46;<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a></p></li></ul></p><p id="par0045" class="elsevierStylePara elsevierViewall">The most commonly used is called the multiple-breath or steady state technique&#44; in which the patient is told to take normal breaths at tidal volume after being connected to a closed system filled with a gas mixture containing 5&#37; He and 0&#46;3&#37; CO&#44; so that the disappearance of CO from the system and the fall in He due to dilution are continuously monitored&#46; During the procedure CO<span class="elsevierStyleInf">2</span> is absorbed&#44; while O<span class="elsevierStyleInf">2</span> is kept between 20&#37; and 22&#37;&#46; The ventilation of the child is measured with a displacement transducer connected to the bellows or piston&#44; which moves as the patient breathes while the He&#44; CO and O<span class="elsevierStyleInf">2</span> concentrations are continuously monitored&#46; As the patient is connected to the system at functional residual capacity &#40;FRC&#41; level&#44; this FRC is determined by He dilution&#44; whereas diffusing capacity is calculated from the exponential decay in CO&#46; The results depend on alveolar ventilation&#44; and the breathing pattern must be as stable as possible&#46;<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a></p><p id="par0050" class="elsevierStylePara elsevierViewall">An inhalation bag with a two-way valve&#44; containing a mixture of CO at a certain concentration&#44; can also be used&#46; The patient breathes several times and the exhaled gas is collected and analysed&#46; The CO uptake is obtained by multiplying the difference between the inhaled and exhaled concentrations by the ventilation volume per minute&#46; The partial alveolar pressure of CO at which the transfer is made also needs to be measured&#46;<a class="elsevierStyleCrossRef" href="#bib0055"><span class="elsevierStyleSup">11</span></a></p><p id="par0055" class="elsevierStylePara elsevierViewall">There are predicted values for DLCO and DLCO&#47;VA with this technique for patients aged from 6 to 18&#46;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a></p><p id="par0060" class="elsevierStylePara elsevierViewall">It is to be expected that the use of new systems&#44; which analyse CO breath by breath&#44; not estimating its concentration but determining it in real time&#44; may avoid errors that arise with the other techniques&#46;</p><p id="par0065" class="elsevierStylePara elsevierViewall">Nevertheless&#44; new validation and standardisation studies of this technique are needed&#46;<ul class="elsevierStyleList" id="lis0010"><li class="elsevierStyleListItem" id="lsti0015"><span class="elsevierStyleLabel">&#40;c&#41;</span><p id="par0070" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Single-breath method</span> &#40;DLCOSB&#41;&#58; measuring DLCO per single breath involves making the patient exhale to residual volume &#40;RV&#41; and then take in a rapid breath of a gas containing CO 0&#46;3&#37;&#44; He 10&#37;&#44; O<span class="elsevierStyleInf">2</span> 21&#37; and nitrogen in equilibrium&#44; to over 90&#37; of vital capacity&#46; After rapid inhalation of the gas&#44; the breath is held for 10<span class="elsevierStyleHsp" style=""></span>s at total lung capacity &#40;TLC&#41; to allow it to diffuse and then the patient is made to exhale rapidly&#46; The first part &#40;between 0&#46;5 and 1<span class="elsevierStyleHsp" style=""></span>L&#41; is discarded&#44; as it corresponds to the dead space that has not undergone the diffusion process&#44; and the second&#44; representing the gas that has been in the alveoli &#40;alveolar fraction&#41;&#44; is used&#46; In this second fraction the final concentration of CO and He is determined&#46; The initial concentration of CO in the alveolar gas is not the same as the concentration of CO in the inhaled gas administered to the patient&#44; since this gas has to be diluted in the air present in the lung after maximum exhalation &#40;RV&#41; and air in the dead space in the system&#46; Since only the concentration of the gas administered is known and not that present in the alveolar space&#44; a tracer gas &#40;generally He&#41; is used to calculate the latter&#46; He is an inert gas&#44; which is not diffused through the alveolar-capillary membrane&#46; Therefore&#44; if we know the initial concentration of He in the gas administered &#40;He<span class="elsevierStyleInf"><span class="elsevierStyleItalic">I</span></span>&#41; and the volume of gas inhaled &#40;measured with the spirometer&#41;&#44; the final concentration of helium &#40;He<span class="elsevierStyleInf"><span class="elsevierStyleItalic">E</span></span>&#41;&#44; measured in the exhaled air&#44; will depend on the final volume in which the He has been dissolved &#40;alveolar volume<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>dead space&#41;&#46;</p></li></ul><elsevierMultimedia ident="eq0010"></elsevierMultimedia>Knowing this final volume we can easily calculate the initial alveolar CO concentration FACO<span class="elsevierStyleInf"><span class="elsevierStyleItalic">i</span></span>&#58;<elsevierMultimedia ident="eq0015"></elsevierMultimedia>Using He also enables us to calculate the alveolar volume &#40;VA&#41;&#44; which will be the volume of air in the alveolar space &#40;that which is diffused&#41;&#46;<elsevierMultimedia ident="eq0020"></elsevierMultimedia>The dead space depends both on the dead space in the system &#40;it must be less than 100<span class="elsevierStyleHsp" style=""></span>mL&#41;&#44; which we know&#44; and on the anatomical dead space &#40;2&#46;2<span class="elsevierStyleHsp" style=""></span>mL&#47;kg of body weight&#41;&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">The main criticism of this method is that it measures diffusion in a very unphysiological situation&#58; during a maximum inhalation and while the breath is held&#44; which does not occur in normal breathing at tidal volume&#46;</p><p id="par0080" class="elsevierStylePara elsevierViewall">Another of the problems with DLCO is that we are using a single value to express the diverse properties of millions of respiratory units&#44; which differ according to the region of the lung&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">Single-breath DLCO depends on the amount of lung tissue involved in the gas exchange&#46; For this reason it is advisable in these patients to assess diffusing capacity per unit of lung volume&#44; KCO &#40;DLCO&#47;VA&#41;&#46;<a class="elsevierStyleCrossRefs" href="#bib0065"><span class="elsevierStyleSup">13&#44;14</span></a></p><p id="par0090" class="elsevierStylePara elsevierViewall">The complexity of the technique&#44; the reference equations for the test&#44; differences in equipment&#44; interpatient variability and the conditions in which the test is performed mean that there is a high degree of interlaboratory variability&#44;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> much greater than with other pulmonary function measurements&#44; and standardisation is therefore essential&#46;<a class="elsevierStyleCrossRefs" href="#bib0010"><span class="elsevierStyleSup">2&#44;8&#44;16&#44;17</span></a> Reference values have been established in healthy white children aged from 5 to 19 for DLCO and VA&#46;<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">18</span></a></p><p id="par0095" class="elsevierStylePara elsevierViewall">We must not forget that the absolute values obtained with these two techniques&#44; single- and multiple-breath&#44; are different&#44; as they are performed at total lung capacity &#40;TLC&#41; and functional residual capacity &#40;FRC&#41; respectively&#44; and diffusion capacity is related to the surface area assessed&#44; which is lower in the latter&#59; therefore when the two techniques are compared the results have to be calculated as z-scores or standard deviations from the corresponding predicted values&#46;</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Practical aspects of the technique</span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Preparation of the patient</span><p id="par0100" class="elsevierStylePara elsevierViewall">The patient must refrain from smoking for 24<span class="elsevierStyleHsp" style=""></span>h before the test&#44; avoid alcohol for at least 4<span class="elsevierStyleHsp" style=""></span>h and not take exercise&#46; He or she will remain seated for at least 5<span class="elsevierStyleHsp" style=""></span>min before the test and throughout the procedure&#46;<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">19</span></a></p><p id="par0105" class="elsevierStylePara elsevierViewall">If the patient requires oxygen it is preferable to discontinue it at least 5<span class="elsevierStyleHsp" style=""></span>min before beginning the test&#46; If this is not possible&#44; the results must be assessed with caution&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Equipment</span><p id="par0110" class="elsevierStylePara elsevierViewall">The patient breathes through a pneumotacograph&#44; which measures the volume of air inhaled and is connected to a three-way valve&#44; which initially allows the patient to breathe ambient air&#59; subsequently&#44; during forced inhalation&#44; the way is opened to the gas cylinder&#44; and during exhalation it is opened to the bag in which the alveolar gas sample is collected&#46; During the 10<span class="elsevierStyleHsp" style=""></span>s for which the breath is held there must be a shutter that prevents exhalation and also a pressure sensor to assess whether the patient is performing Valsalva manoeuvres &#40;exhalation&#41; or M&#252;ller&#39;s manoeuvres &#40;inhalation&#41;&#44; which modify pulmonary capillary blood volume&#46; The Valsalva manoeuvre reduces DLCO and M&#252;ller&#39;s manoeuvre increases it&#46;<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">20</span></a> Finally&#44; gas analysers are needed to ascertain the concentration of exhaled CO and He&#46; The system must have a dead space of less than 100<span class="elsevierStyleHsp" style=""></span>mL&#46; All the apparatuses must be calibrated daily&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a></p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Test procedure</span><p id="par0115" class="elsevierStylePara elsevierViewall"><ul class="elsevierStyleList" id="lis0015"><li class="elsevierStyleListItem" id="lsti0020"><span class="elsevierStyleLabel">1&#41;</span><p id="par0120" class="elsevierStylePara elsevierViewall">Explain the manoeuvre&#46; It is advisable to carry out a simulation first without gas inhalation&#46;</p></li><li class="elsevierStyleListItem" id="lsti0025"><span class="elsevierStyleLabel">2&#41;</span><p id="par0125" class="elsevierStylePara elsevierViewall">The mouthpiece is placed in the mouth and the clip on the nose and the patient is asked to breathe calmly&#46;</p></li><li class="elsevierStyleListItem" id="lsti0030"><span class="elsevierStyleLabel">3&#41;</span><p id="par0130" class="elsevierStylePara elsevierViewall">The patient is told to exhale to RV&#46; If there is a major obstructive disease it is recommended that exhalation should be limited to 6<span class="elsevierStyleHsp" style=""></span>s&#46;</p></li><li class="elsevierStyleListItem" id="lsti0035"><span class="elsevierStyleLabel">4&#41;</span><p id="par0135" class="elsevierStylePara elsevierViewall">A rapid breath is taken to TLC&#58;<ul class="elsevierStyleList" id="lis0020"><li class="elsevierStyleListItem" id="lsti0040"><span class="elsevierStyleLabel">a&#41;</span><p id="par0140" class="elsevierStylePara elsevierViewall">The inspiratory volume &#40;IV&#41; must be at least 90&#37; of the largest previous VC &#40;spirometry must be performed beforehand as a guide&#41;&#46;</p></li><li class="elsevierStyleListItem" id="lsti0045"><span class="elsevierStyleLabel">b&#41;</span><p id="par0145" class="elsevierStylePara elsevierViewall">The inspiration must be rapid enough for 90&#37; of the IV to be inhaled within 1&#46;5&#8211;2<span class="elsevierStyleHsp" style=""></span>s in healthy individuals and in less than 4<span class="elsevierStyleHsp" style=""></span>s in patients with obstructive disease&#46; If the inhalation lasts for 5<span class="elsevierStyleHsp" style=""></span>s&#44; DLCO increases by around 13&#37;&#46;</p></li></ul></p></li><li class="elsevierStyleListItem" id="lsti0050"><span class="elsevierStyleLabel">5&#41;</span><p id="par0150" class="elsevierStylePara elsevierViewall">The breath must be held for 10<span class="elsevierStyleHsp" style=""></span>s&#46;<ul class="elsevierStyleList" id="lis0025"><li class="elsevierStyleListItem" id="lsti0055"><span class="elsevierStyleLabel">a&#41;</span><p id="par0155" class="elsevierStylePara elsevierViewall">During this time no expiratory or inspiratory effort must be made against the shutter&#46;</p></li></ul></p></li><li class="elsevierStyleListItem" id="lsti0060"><span class="elsevierStyleLabel">6&#41;</span><p id="par0160" class="elsevierStylePara elsevierViewall">After this the patient must exhale rapidly&#46;<ul class="elsevierStyleList" id="lis0030"><li class="elsevierStyleListItem" id="lsti0065"><span class="elsevierStyleLabel">a&#41;</span><p id="par0165" class="elsevierStylePara elsevierViewall">The first part &#40;about 750<span class="elsevierStyleHsp" style=""></span>mL&#41; is discarded&#46; If the patient has a VC<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>2<span class="elsevierStyleHsp" style=""></span>L this can be reduced to 500<span class="elsevierStyleHsp" style=""></span>mL&#46;</p></li><li class="elsevierStyleListItem" id="lsti0070"><span class="elsevierStyleLabel">b&#41;</span><p id="par0170" class="elsevierStylePara elsevierViewall">The volume analysed must be between 500 and 1<span class="elsevierStyleHsp" style=""></span>000<span class="elsevierStyleHsp" style=""></span>mL and must be exhaled in less than 4<span class="elsevierStyleHsp" style=""></span>s&#46;</p></li></ul></p></li><li class="elsevierStyleListItem" id="lsti0075"><span class="elsevierStyleLabel">7&#41;</span><p id="par0175" class="elsevierStylePara elsevierViewall">The patient is told to remove the nose clip and remain seated&#46;</p></li><li class="elsevierStyleListItem" id="lsti0080"><span class="elsevierStyleLabel">8&#41;</span><p id="par0180" class="elsevierStylePara elsevierViewall">The test must be repeated until at least two values are achieved that agree within 10&#37; and below 3<span class="elsevierStyleHsp" style=""></span>mL&#47;min&#47;mmHg&#46; At least 4<span class="elsevierStyleHsp" style=""></span>min must elapse between each test&#46; In patients with major obstruction it may be necessary to wait up to 10<span class="elsevierStyleHsp" style=""></span>min between tests for the gases to be completely eliminated&#46; It may be useful to ask the patient to take deep breaths so as to eliminate the gas more effectively&#46;</p></li><li class="elsevierStyleListItem" id="lsti0085"><span class="elsevierStyleLabel">9&#41;</span><p id="par0185" class="elsevierStylePara elsevierViewall">Calculations&#58;</p></li></ul><elsevierMultimedia ident="eq0025"></elsevierMultimedia>VA<span class="elsevierStyleInf">STPD</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>alveolar volume at STPD&#59; 60&#47;<span class="elsevierStyleItalic">t</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>breath-hold time converted into minutes&#59; PB&#8211;47<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>barometric pressure minus water vapour pressure at 37<span class="elsevierStyleHsp" style=""></span>&#176;C&#59; ln<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>natural logarithm&#59; FA<span class="elsevierStyleSmallCaps">CO</span><span class="elsevierStyleInf">o</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>alveolar CO concentration at the start of diffusion&#59; FACO<span class="elsevierStyleInf"><span class="elsevierStyleItalic">t</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>alveolar CO concentration at the end of the test&#46;<elsevierMultimedia ident="eq0030"></elsevierMultimedia>He<span class="elsevierStyleInf"><span class="elsevierStyleItalic">E</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>exhaled He concentration&#59; He<span class="elsevierStyleInf"><span class="elsevierStyleItalic">I</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>inhaled He concentration&#59; CO<span class="elsevierStyleInf"><span class="elsevierStyleItalic">I</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>inhaled CO concentration&#59; CO<span class="elsevierStyleInf"><span class="elsevierStyleItalic">E</span></span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>exhaled CO concentration&#46;</p><p id="par0190" class="elsevierStylePara elsevierViewall">Given that diffusing capacity is calculated per unit of time &#40;mL of CO absorbed per minute&#41;&#44; exact calculation of the time the patient holds his or her breath&#44; which is the time during which CO diffusion takes place&#44; is crucial&#46;<a class="elsevierStyleCrossRefs" href="#bib0110"><span class="elsevierStyleSup">22&#44;23</span></a> The Jones&#8211;Meade method for measuring the time starts counting from 30&#37; of the inhalation time and stops at 50&#37; of the alveolar gas sample collection time &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><p id="par0195" class="elsevierStylePara elsevierViewall">In patients with obstruction it may be preferable to perform the test after using a bronchodilator&#44; although if heart rate increases&#44; volume per minute also increases&#44; raising DLCO&#46; For this reason it is advisable to carry out the test at least 30<span class="elsevierStyleHsp" style=""></span>min after administering the bronchodilator&#46;</p></span></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Adjustments</span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Adjustment for haemoglobin &#40;Hb&#41;</span><p id="par0200" class="elsevierStylePara elsevierViewall">Patients with anaemia have a reduced DLCO because CO uptake by erythrocytes is lower&#46; The Cotes method<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a> of correction for Hb makes it possible to calculate adjusted DLCO in adult males with Hb greater than 14&#46;6<span class="elsevierStyleHsp" style=""></span>g&#47;dL or in women and in children under 15 with Hb<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>13&#46;4<span class="elsevierStyleHsp" style=""></span>g&#47;dL&#46;</p><p id="par0205" class="elsevierStylePara elsevierViewall">Males 15 years of age and older&#58;<elsevierMultimedia ident="eq0035"></elsevierMultimedia>Children aged &#60;15 years and women&#58;<elsevierMultimedia ident="eq0040"></elsevierMultimedia>It is advisable to put both values in the report&#58; the measured DLCO and the DLCO adjusted for Hb&#46;</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Clinical applications</span><p id="par0210" class="elsevierStylePara elsevierViewall">CO transfer can be either increased or reduced in various processes &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">8</span></a></p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0215" class="elsevierStylePara elsevierViewall">CO transfer is increased in situations in which there is an increase in blood volume in the pulmonary capillaries &#40;exercise&#44; left-right shunts&#41;&#44; polycythaemia and pulmonary haemorrhage &#40;falsely elevated DLCO&#41;&#46; Occasionally there is a rise in DLCO in asthmatics&#44; due to an increase in pulmonary blood volume though negative intrathoracic pressure produced after rapid inhalations&#46;</p><p id="par0220" class="elsevierStylePara elsevierViewall">DLCO is decreased in patients with a reduction in alveolar volume or in diffusion defects &#40;<span class="elsevierStyleItalic">alteration of the alveolar-capillary membrane</span> &#91;interstitial lung disease&#58; idiopathic pulmonary fibrosis&#44; extrinsic allergic alveolitis&#44; scleroderma&#44; sarcoidosis&#44; asbestosis&#93; or <span class="elsevierStyleItalic">decreased pulmonary capillary blood volume</span> &#91;pulmonary embolism or primary pulmonary hypertension&#93;&#41;&#46; In pulmonary emphysema DLCO is characteristically reduced by loss of alveolar-capillary membrane surface area secondary to alveolar rupture&#44; giving rise to an increase in TLC with a decreased KCO &#40;DLCO&#47;VA&#41;&#46; In congestive heart failure the reduction in DLCO may be secondary to interstitial oedema&#46; Other causes of decreased DLCO are anaemia&#44; renal failure&#44; smoking and marijuana use&#46;</p><p id="par0225" class="elsevierStylePara elsevierViewall">In patients with restrictive diseases&#44; especially in children with deformities of the rib cage or neuromuscular diseases in which normal lungs are restrictive because of deformity or muscular weakness&#44; the surface area for diffusion is relatively large per unit of pulmonary volume&#44; and for this reason DLCO may be normal&#46; The patient&#39;s DLCO&#47;VA should therefore be compared with reference values based on his or her &#40;restrictive&#41; TLC rather than his or her theoretical TLC&#46;<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">25</span></a></p><p id="par0230" class="elsevierStylePara elsevierViewall">In cystic fibrosis both raising and lowering of DLCO may be found&#46; Initially the transfer factor may be elevated by an increase in the amount of blood reaching the lung&#44; due to fluctuations in pleural pressure secondary to bronchial obstruction&#59; later&#44; with the onset of pulmonary heart disease&#44; pulmonary microcirculation is impaired and diffusion is gradually reduced&#46;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a></p><p id="par0235" class="elsevierStylePara elsevierViewall">Indications for the DLCO test are set out in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a></p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0240" class="elsevierStylePara elsevierViewall">In practice the main indications in paediatrics are as follows<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a>&#58;</p><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Monitoring of treatments that are toxic to the lungs</span><p id="par0245" class="elsevierStylePara elsevierViewall">Chemotherapy &#40;methotrexate&#44; nitrofurantoin&#44; azathioprine&#44; penicillamine&#44; cyclophosphamide and especially bleomycin&#41; can produce a major decrease in DLCO&#44; which must be monitored&#46;</p><p id="par0250" class="elsevierStylePara elsevierViewall">Chest radiation can cause irreversible diffusion impairment&#46;</p><p id="par0255" class="elsevierStylePara elsevierViewall">Treatments for autoimmune or rheumatological diseases can also impair pulmonary diffusion&#46;</p><p id="par0260" class="elsevierStylePara elsevierViewall">Immunosuppressants used after organ transplants have been associated with obstructive and restrictive lung disease&#46;</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Diagnosis and monitoring of patients with chronic interstitial lung disease</span><p id="par0265" class="elsevierStylePara elsevierViewall">Impairment of diffusing capacity is one of the initial signs of interstitial lung disease and a fundamental indicator of clinical course and response to treatment&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Monitoring of children with diseases that cause pulmonary bleeding</span><p id="par0270" class="elsevierStylePara elsevierViewall">In primary pulmonary haemosiderosis&#44; Goodpasture syndrome and granulomatosis with polyangiitis&#44; an increase in diffusing capacity can predict relapse or indicate progression in asymptomatic patients&#46; Moreover&#44; pulmonary haemosiderosis that does not respond to treatment may eventually give rise to pulmonary fibrosis&#44; leading to decreased diffusion&#46;</p></span></span></span>"
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          "titulo" => "Introduction"
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          "identificador" => "sec0010"
          "titulo" => "Techniques for measuring diffusion"
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          "identificador" => "sec0015"
          "titulo" => "Practical aspects of the technique"
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              "identificador" => "sec0020"
              "titulo" => "Preparation of the patient"
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              "titulo" => "Equipment"
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              "identificador" => "sec0030"
              "titulo" => "Test procedure"
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          "titulo" => "Adjustments"
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              "titulo" => "Adjustment for haemoglobin &#40;Hb&#41;"
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          "titulo" => "Clinical applications"
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              "titulo" => "Monitoring of treatments that are toxic to the lungs"
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            1 => array:2 [
              "identificador" => "sec0055"
              "titulo" => "Diagnosis and monitoring of patients with chronic interstitial lung disease"
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            2 => array:2 [
              "identificador" => "sec0060"
              "titulo" => "Monitoring of children with diseases that cause pulmonary bleeding"
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          "titulo" => "References"
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    "pdfFichero" => "main.pdf"
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    "fechaRecibido" => "2014-09-29"
    "fechaAceptado" => "2014-10-30"
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        0 => array:4 [
          "clase" => "keyword"
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          "palabras" => array:3 [
            0 => "Lung diffusion capacity"
            1 => "Single breath technique"
            2 => "Practical aspects"
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        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec560882"
          "palabras" => array:3 [
            0 => "Capacidad de difusi&#243;n"
            1 => "T&#233;cnica de la respiraci&#243;n &#250;nica"
            2 => "Aplicaciones cl&#237;nicas"
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        ]
      ]
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    "resumen" => array:2 [
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">The diffusion capacity is the technique that measures the ability of the respiratory system for gas exchange&#44; thus allowing a diagnosis of the malfunction of the alveolar-capillary unit&#46; The most important parameter to assess is the CO diffusion capacity &#40;DLCO&#41;&#46; New methods are currently being used to measure the diffusion using nitric oxide &#40;NO&#41;&#46; There are other methods for measuring diffusion&#44; although in this article the single breath technique is mainly referred to&#44; as it is the most widely used and best standardised&#46;</p><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Its complexity&#44; its reference equations&#44; differences in equipment&#44; inter-patient variability and conditions in which the DLCO is performed&#44; lead to a wide inter-laboratory variability&#44; although its standardisation makes this a more reliable and reproductive method&#46;</p><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The practical aspects of the technique are analysed&#44; by specifying the recommendations to carry out a suitable procedure&#44; the calibration routine&#44; calculations and adjustments&#46; Clinical applications are also discussed&#46; An increase in the transfer of CO occurs in diseases in which there is an increased volume of blood in the pulmonary capillaries&#44; such as in polycythemia and pulmonary haemorrhage&#46; There is a decrease in DLCO in patients with alveolar volume reduction or diffusion defects&#44; either by altered alveolar-capillary membrane &#40;interstitial diseases&#41; or decreased volume of blood in the pulmonary capillaries &#40;pulmonary embolism or primary pulmonary hypertension&#41;&#46; Other causes of decreased or increased DLCO are also highlighted&#46;</p></span>"
      ]
      "es" => array:2 [
        "titulo" => "Resumen"
        "resumen" => "<span id="abst0010" class="elsevierStyleSection elsevierViewall"><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">La capacidad de difusi&#243;n es la t&#233;cnica que mide la capacidad del aparato respiratorio para realizar el intercambio gaseoso y as&#237; diagnosticar la disfunci&#243;n de la unidad alv&#233;olo-capilar&#46;</p><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">El par&#225;metro m&#225;s importante a evaluar es la capacidad de difusi&#243;n del CO &#40;DLCO&#41;&#46; Actualmente hay nuevos m&#233;todos para medir la capacidad de difusi&#243;n utilizando &#243;xido n&#237;trico &#40;NO&#41;&#46; Existen diferentes m&#233;todos de medida&#44; aunque en este art&#237;culo nos referiremos sobre todo a la t&#233;cnica de la respiraci&#243;n &#250;nica&#44; la m&#225;s utilizada y mejor estandarizada&#46;</p><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Su complejidad&#44; sus ecuaciones de referencia&#44; las diferencias en equipamiento&#44; la variabilidad interpacientes y las condiciones en las que se realiza hacen que exista una gran variabilidad interlaboratorio&#44; habi&#233;ndose realizado estandarizaciones para hacer este m&#233;todo m&#225;s fiable y reproducible&#46;</p><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Se analizan los aspectos pr&#225;cticos de la t&#233;cnica&#44; especificando las recomendaciones para la realizaci&#243;n de un procedimiento adecuado&#44; sistem&#225;tica de calibraci&#243;n y c&#225;lculos y ajustes necesarios&#46; Tambi&#233;n se exponen sus aplicaciones cl&#237;nicas&#46;</p><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Se produce un aumento de la transferencia de CO en las enfermedades en las que existe un aumento del volumen sangu&#237;neo en los capilares pulmonares&#44; en la policitemia y en la hemorragia pulmonar&#46; Existe una disminuci&#243;n de la DLCO en los pacientes con reducci&#243;n del volumen alveolar o en los defectos de difusi&#243;n&#44; ya sea por alteraci&#243;n de la membrana alv&#233;olo-capilar &#40;enfermedad intersticial&#41; o por disminuci&#243;n del volumen de sangre en los capilares pulmonares &#40;embolia pulmonar o hipertensi&#243;n pulmonar primaria&#41;&#46; Tambi&#233;n se exponen otras causas de disminuci&#243;n o aumento de la DLCO&#46;</p></span>"
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        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Please cite this article as&#58; Posadas AS&#44; Asensi JRV&#44; de MirMessa I&#44; Prado OS&#44; Larramona H&#46; Medici&#243;n de la difusi&#243;n de CO &#40;II&#41;&#58; estandarizaci&#243;n y criterios de calidad&#46; An Pediatr &#40;Barc&#41;&#46; 2015&#59;83&#58;137&#46;e1&#8211;137&#46;e7&#46;</p>"
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                  \t\t\t\t" class=""><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Extrapulmonary reduction in alveolar volume</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Reduced respiratory effort or muscular debility&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Thoracic deformities preventing full inflation of the thorax&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Situations involving reduction of the CO-Hb reaction</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Anaemia&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary embolism&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Changes in haemoglobin&#39;s binding properties &#40;carboxyhaemoglobin&#44; increased FiO2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Conditions that alter the alveolar-capillary membrane and the CO-Hb binding&#44; reducing DLCO</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary resection &#40;there may be a compensating increase in the CO-Hb reaction&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Emphysema&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Interstitial lung disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary oedema&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary vasculitis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary hypertension&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Situations that increase the CO-Hb reaction and raise DLCO</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Polycythaemia&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Left-right shunt&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary haemorrhage&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Asthma&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Changes in the Hb binding properties &#40;reduced FiO2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>M&#252;ller&#39;s manoeuvre &#40;reduction in intrathoracic pressure&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t" class=""><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">1&#46; Evaluation and followup of diseases affecting lung parenchyma &#40;those associated with drug reactions&#44; pneumoconiosis or sarcoidosis&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">2&#46; Evaluation and followup of emphysema&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">3&#46; Differentiating between chronic bronchitis&#44; emphysema and asthma&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">4&#46; Assessment of pulmonary involvement in systemic diseases&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">5&#46; Assessment of cardiovascular diseases&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">6&#46; Prediction of arterial desaturation during exercise in some patients with lung disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">7&#46; Assessment and quantification of the degree of disability associated with pulmonary fibrosis or with emphysema&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">8&#46; Assessment of the pulmonary effects of chemotherapy agents or other drugs that induce pulmonary dysfunction&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">9&#46; Assessment of pulmonary haemorrhage&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">10&#46; As an early indicator of certain pulmonary infections that cause diffuse pneumonitis &#40;e&#46;g&#46;&#44; <span class="elsevierStyleItalic">Pneumocystis</span> pneumonia&#41;&nbsp;\t\t\t\t\t\t\n
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                            3 => "D&#46;C&#46; Johnson"
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                          0 => array:2 [
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                            4 => "J&#46;W&#46; Lammers"
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                    ]
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                      "titulo" => "Lung diffusing capacity for nitric oxide and carbon monoxide in relation to morphological changes as assessed by computed tomography in patients with cystic fibrosis"
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                        "fecha" => "2009"
                        "volumen" => "9"
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                        "link" => array:1 [
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                            5 => "R&#46;M&#46; Huber"
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                        "tituloSerie" => "Chest"
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                        "volumen" => "133"
                        "paginaInicial" => "1149"
                        "paginaFinal" => "1154"
                        "link" => array:1 [
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                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18263682"
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              "etiqueta" => "7"
              "referencia" => array:1 [
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                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "European reference equations for CO and NO lung transfer"
                      "autores" => array:1 [
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                          "etal" => false
                          "autores" => array:5 [
                            0 => "B&#46; Aguilaniu"
                            1 => "J&#46; Maitre"
                            2 => "S&#46; Gle¿net"
                            3 => "A&#46; Gegout-Petit"
                            4 => "H&#46; Gue¿nard"
                          ]
                        ]
                      ]
                    ]
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                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1183/09031936.00063207"
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                        "tituloSerie" => "Eur Respir J"
                        "fecha" => "2008"
                        "volumen" => "31"
                        "paginaInicial" => "1091"
                        "paginaFinal" => "1097"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18216061"
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                          ]
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              "identificador" => "bib0040"
              "etiqueta" => "8"
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                      "titulo" => "Single-breath carbon monoxide diffusing capacity &#40;transfer factor&#41;&#46; Recommendations for a standard technique&#58; 1995 update"
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                          "autores" => array:1 [
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                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "1995"
                        "volumen" => "152"
                        "numero" => "6 Pt 1"
                        "paginaInicial" => "2185"
                        "paginaFinal" => "2198"
                        "link" => array:1 [
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              "identificador" => "bib0045"
              "etiqueta" => "9"
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                      "titulo" => "Comparison of carbon monoxide &#40;CO&#41; single breath pulmonary diffusing capacity with non-rebreathing&#44; open-circuit CO pulmonary diffusing capacity in healthy children"
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                          "etal" => false
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                            1 => "S&#46; Thomas"
                            2 => "K&#46;C&#46; Beck"
                            3 => "R&#46;P&#46; Mlcak"
                            4 => "D&#46;N&#46; Herndon"
                          ]
                        ]
                      ]
                    ]
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                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1002/ppul.20504"
                      "Revista" => array:6 [
                        "tituloSerie" => "Pediatr Pulmonol"
                        "fecha" => "2006"
                        "volumen" => "41"
                        "paginaInicial" => "1095"
                        "paginaFinal" => "1102"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16986167"
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                          ]
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                      ]
                    ]
                  ]
                ]
              ]
            ]
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              "identificador" => "bib0050"
              "etiqueta" => "10"
              "referencia" => array:1 [
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                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lung diffusing capacity"
                      "autores" => array:1 [
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                          "autores" => array:3 [
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                            1 => "P&#46;J&#46;F&#46;M&#46; Merkus"
                            2 => "H&#46; Stam"
                          ]
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                        "serieTitulo" => "Paediatric pulmonary function testing&#46; Vol&#46; 33&#46; Prog Respir Res"
                        "serieFecha" => "2005"
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              "identificador" => "bib0055"
              "etiqueta" => "11"
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                      "titulo" => "Capacidad de difusi&#243;n"
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                            0 => "N&#46; Gonz&#225;lez Mangado"
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                        "titulo" => "Exploraci&#243;n funcional respiratoria&#46; Monograf&#237;as Neumo&#46; Vol XVIII&#47;2011"
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                        "paginaFinal" => "58"
                        "serieFecha" => "2011"
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                    ]
                  ]
                ]
              ]
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              "identificador" => "bib0060"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "A rebreathing method to determine carbon monoxide diffusing capacity in children&#58; reference values for 6- to 18-year-olds &#91;corrected&#93; and validation in adult volunteers"
                      "autores" => array:1 [
                        0 => array:2 [
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                            1 => "A&#46;V&#46; Beek"
                            2 => "K&#46; Gr&#252;nberg"
                            3 => "M&#46;A&#46; de Ridder"
                            4 => "J&#46;C&#46; de Jongste"
                            5 => "A&#46; Versprille"
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                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Pediatr Pulmonol"
                        "fecha" => "1998"
                        "volumen" => "25"
                        "paginaInicial" => "205"
                        "paginaFinal" => "212"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9556013"
                            "web" => "Medline"
                          ]
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              "identificador" => "bib0065"
              "etiqueta" => "13"
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                          "autores" => array:1 [
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                          ]
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                        "serieFecha" => "2009"
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                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2012"
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                        "paginaInicial" => "132"
                        "paginaFinal" => "139"
                        "link" => array:1 [
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                            "web" => "Medline"
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              "etiqueta" => "15"
              "referencia" => array:1 [
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                          "autores" => array:1 [
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                    0 => array:2 [
                      "doi" => "10.4187/respcare.01435"
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                        "volumen" => "57"
                        "paginaInicial" => "17"
                        "paginaFinal" => "23"
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                          0 => array:2 [
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              "identificador" => "bib0080"
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              "referencia" => array:1 [
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                      "titulo" => "Standardization of the measurement of transfer factor"
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                        "paginaFinal" => "1578"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8112455"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            16 => array:3 [
              "identificador" => "bib0085"
              "etiqueta" => "17"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:1 [
                      "titulo" => "Guidelines for the measurement of respiratory function&#46; Recommendations of the British Thoracic Society and the Association of Respiratory Technicians and Physiologists"
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "Respir Med"
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                        "numero" => "3"
                        "paginaInicial" => "165"
                        "paginaFinal" => "194"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8209067"
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                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
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              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pulmonary diffusing capacity in healthy Caucasian children"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Y&#46;J&#46; Kim"
                            1 => "G&#46;L&#46; Hall"
                            2 => "K&#46; Christoph"
                            3 => "R&#46; Tabbey"
                            4 => "Z&#46; Yu"
                            5 => "R&#46;S&#46; Tepper"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1002/ppul.21564"
                      "Revista" => array:6 [
                        "tituloSerie" => "Pediatr Pulmonol"
                        "fecha" => "2012"
                        "volumen" => "47"
                        "paginaInicial" => "469"
                        "paginaFinal" => "475"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22081537"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            18 => array:3 [
              "identificador" => "bib0095"
              "etiqueta" => "19"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Normal values for single exhalation diffusing capacity and pulmonary capillary blood flow in sitting&#44; supine positions&#44; and during mild exercise"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
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                            2 => "N&#46;R&#46; MacIntyre"
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                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
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                        "tituloSerie" => "Chest"
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                        "volumen" => "105"
                        "paginaInicial" => "501"
                        "paginaFinal" => "508"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8306754"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
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              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effect of high negative inspiratory pressure on single breath CO diffusing capacity"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "D&#46;J&#46; Cotton"
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                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Respir Physiol"
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                        "paginaInicial" => "19"
                        "paginaFinal" => "29"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/6648064"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
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              "etiqueta" => "21"
              "referencia" => array:1 [
                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "Single-breath carbon monoxide diffusing capacity &#40;DLCO&#41; or transfer factor"
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                          "etal" => false
                          "autores" => array:1 [
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                    ]
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                      "LibroEditado" => array:2 [
                        "titulo" => "Pulmonary function laboratory mangement and procedure manual&#46; A project of the American Thoracic Society"
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                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
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              "etiqueta" => "22"
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                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "A standardized breath holding technique for the clinical measurement of the diffusing capacity of the lung for carbon monoxide"
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                  "host" => array:1 [
                    0 => array:2 [
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                        "tituloSerie" => "J Clin Invest"
                        "fecha" => "1957"
                        "volumen" => "36"
                        "numero" => "1 Part 1"
                        "paginaInicial" => "1"
                        "paginaFinal" => "17"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16695816"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
            ]
            22 => array:3 [
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              "etiqueta" => "23"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "A theoretical and experimental analysis of anomalies in the estimation of pulmonary diffusing capacity by the single breath method"
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                          "etal" => false
                          "autores" => array:2 [
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                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Q J Exp Physiol Cogn Med Sci"
                        "fecha" => "1961"
                        "volumen" => "46"
                        "paginaInicial" => "131"
                        "paginaFinal" => "143"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/13790588"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            23 => array:3 [
              "identificador" => "bib0120"
              "etiqueta" => "24"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Iron-deficiency anaemia&#58; Its effect on transfer factor for the lung &#40;diffusion capacity&#41; and ventilation and cardiac frequency during sub-maximal exercise"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
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                            1 => "J&#46;M&#46; Dabbs"
                            2 => "P&#46;C&#46; Elwood"
                            3 => "A&#46;M&#46; Hall"
                            4 => "A&#46; McDonald"
                            5 => "M&#46;J&#46; Saunders"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Sci"
                        "fecha" => "1972"
                        "volumen" => "42"
                        "paginaInicial" => "325"
                        "paginaFinal" => "335"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/5013875"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
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              "etiqueta" => "25"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pulmonary diffusing capacity at reduced alveolar volumes in children"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "H&#46; Stam"
                            1 => "B&#46;A&#46; van den"
                            2 => "K&#46; Grunberg"
                            3 => "T&#46; Stijnen"
                            4 => "H&#46;A&#46; Tiddens"
                            5 => "A&#46; Versprille"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1002/(SICI)1099-0496(199602)21:2<84::AID-PPUL4>3.0.CO;2-P"
                      "Revista" => array:6 [
                        "tituloSerie" => "Pediatr Pulmonol"
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                        "paginaInicial" => "84"
                        "paginaFinal" => "89"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8882211"
                            "web" => "Medline"
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                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
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              "identificador" => "bib0130"
              "etiqueta" => "26"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Reduction of the single breath CO diffusing capacity in cystic fibrosis"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "D&#46;J&#46; Cotton"
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                            3 => "B&#46;F&#46; Habbick"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
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                      "Revista" => array:6 [
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                        "paginaFinal" => "222"
                        "link" => array:1 [
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