Original articleProtective effects of Y-27632 on hypoxia/reoxygenation-induced intestinal injury in newborn rats
Section snippets
The animals
The investigation conforms with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996). The experimental protocol was reviewed and approved by the local ethics committee of the University of Gaziantep. The study was carried out on 1-day-old Wistar albino rat pups whose mothers were housed at 22°C to 24°C with a 12-hour light/12-hour dark cycle and supplied with standard rat chow and water ad libitum.
Experimental design
A
Histopathology
The microscopic lesions in H/R rat pups were similar to those seen in neonatal NEC, with severe destruction of villi and crypts (Fig. 1). Nonhypoxic rat pups had essentially no intestinal tract alteration. The results of the histopathologic examinations are shown in Fig. 2. Intestinal injury score of the H/R group was significantly higher when compared with the nonhypoxic untreated controls. Y-27632 treatment following H/R led to significant decrease in the intestinal injury score. The scores
Discussion
In this experimental study, H/R resulted in marked elevation in tissue MDA levels and decreased tissue NO levels in rat pup intestine. Histologic examination revealed that H/R led to injuries in the intestine, which were similar to those seen in neonatal NEC. The major novel finding of our study was that Y-27632 treatment after H/R injury demonstrated beneficial effects on H/R-induced intestinal injury in newborn rats. Y-27632 significantly decreased the severity of histologic lesions, resulted
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